C57BL/6JCya-Dnase2aem1flox/Cya
Common Name:
Dnase2a-flox
Product ID:
S-CKO-02066
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Dnase2a-flox
Strain ID
CKOCMP-13423-Dnase2a-B6J-VA
Gene Name
Product ID
S-CKO-02066
Gene Alias
Dnase2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dnase2aem1flox/Cya mice (Catalog S-CKO-02066) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000003910
NCBI RefSeq
NM_010062
Target Region
Exon 1~5
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Dnase2a, also known as DNase II, is a lysosomal nuclease. Its essential function is to degrade DNA, participating in multiple biological processes. It is involved in the phagolysosomal degradation of apoptotic cell DNA, and the nucleotides derived from this hydrolysis can activate the DNA-PKcs-mTORC2/Rictor pathway, promoting non-inflammatory macrophage proliferation [1]. It also plays a role in the clearance of damaged nuclear DNA in non-phagocytic cells, with this process requiring nuclear export and autophagy-mediated delivery of DNA to lysosomes [2].
In gene knockout mouse models, Dnase2a deficiency leads to elevated levels of undegraded DNA in cells. In non-phagocytic cells, this excess DNA comes from damaged nuclear DNA. The accumulated DNA can induce inflammation via the Sting cytosolic DNA-sensing pathway [2]. In atherosclerosis regression, silencing macrophage Dnase2a blocks efferocyte proliferation, apoptotic cell clearance, and plaque stabilization [1]. In definitive erythropoiesis, Dnase2a KO mice suffer from severe anemia and die in utero, as it is crucial for digesting the DNA of extruded nuclei of red blood cells during maturation [3].
In conclusion, Dnase2a is vital for DNA degradation in various biological contexts. Its deficiency in KO mouse models reveals its importance in processes like apoptotic cell clearance, macrophage proliferation, and erythropoiesis, with implications for diseases such as atherosclerosis and anemia. Understanding Dnase2a through these models provides insights into the underlying mechanisms of these biological processes and disease conditions.
References:
1. Gerlach, Brennan D, Ampomah, Patrick B, Yurdagul, Arif, Tao, Wei, Tabas, Ira. 2021. Efferocytosis induces macrophage proliferation to help resolve tissue injury. In Cell metabolism, 33, 2445-2463.e8. doi:10.1016/j.cmet.2021.10.015. https://pubmed.ncbi.nlm.nih.gov/34784501/
2. Lan, Yuk Yuen, Londoño, Diana, Bouley, Richard, Rooney, Michael S, Hacohen, Nir. 2014. Dnase2a deficiency uncovers lysosomal clearance of damaged nuclear DNA via autophagy. In Cell reports, 9, 180-192. doi:10.1016/j.celrep.2014.08.074. https://pubmed.ncbi.nlm.nih.gov/25284779/
3. Porcu, Susanna, Manchinu, Maria F, Marongiu, Maria F, Grosveld, Frank, Ristaldi, Maria S. 2011. Klf1 affects DNase II-alpha expression in the central macrophage of a fetal liver erythroblastic island: a non-cell-autonomous role in definitive erythropoiesis. In Molecular and cellular biology, 31, 4144-54. doi:10.1128/MCB.05532-11. https://pubmed.ncbi.nlm.nih.gov/21807894/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen