C57BL/6JCya-S1pr4em1flox/Cya
Common Name:
S1pr4-flox
Product ID:
S-CKO-02138
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
S1pr4-flox
Strain ID
CKOCMP-13611-S1pr4-B6J-VA
Gene Name
Product ID
S-CKO-02138
Gene Alias
Edg6; Lpc1; S1p4
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-S1pr4em1flox/Cya mice (Catalog S-CKO-02138) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000053646
NCBI RefSeq
NM_010102
Target Region
Exon 1
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
S1pr4, a G protein-coupled receptor, is one of the five subtypes of sphingosine 1-phosphate receptors. Sphingosine 1-phosphate (S1P) binds to S1pr4, and this interaction is involved in regulating immune cell biology, including the trafficking of lymphocytes, neutrophils, and other cells. Signaling through S1pr4 is associated with multiple pathways, such as the NF-κB pathway, and is of great importance in immune-related biological processes and disease conditions [2].
In murine models, S1pr4-deficient mice showed reduced psoriasis severity, with attenuated production of CCL2, IL-6, and CXCL1, and a decrease in infiltrating monocytes and granulocytes, indicating its role in macrophage recruitment in psoriasis [1]. Ablation of S1pr4 in murine models of mammary and colitis-associated colorectal cancer delayed tumor development and improved therapy success through increased CD8+ T cell abundance, suggesting a tumor-promoting role of S1pr4 by restricting CD8+ T cell expansion [3]. In a murine asthma model, S1pr4 deficiency aggravated OVA/LPS-induced pulmonary inflammation with up-regulation in M1 macrophage activation, highlighting its potential as a therapeutic target for non-eosinophilic asthma [4].
In conclusion, S1pr4 plays crucial roles in immune-related biological processes. Gene-knockout mouse models have revealed its functions in diseases like psoriasis, cancer, and asthma. Understanding S1pr4 can provide new insights into the mechanisms of these diseases and potential therapeutic strategies.
References:
1. Schuster, Christian, Huard, Arnaud, Sirait-Fischer, Evelyn, Brüne, Bernhard, Weigert, Andreas. 2020. S1PR4-dependent CCL2 production promotes macrophage recruitment in a murine psoriasis model. In European journal of immunology, 50, 839-845. doi:10.1002/eji.201948349. https://pubmed.ncbi.nlm.nih.gov/32017036/
2. Chen, Hongyu, Wang, Junmin, Zhang, Caiyun, Ji, Guang, Wu, Tao. 2022. Sphingosine 1-phosphate receptor, a new therapeutic direction in different diseases. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 153, 113341. doi:10.1016/j.biopha.2022.113341. https://pubmed.ncbi.nlm.nih.gov/35785704/
3. Olesch, Catherine, Sirait-Fischer, Evelyn, Berkefeld, Matthias, Brüne, Bernhard, Weigert, Andreas. . S1PR4 ablation reduces tumor growth and improves chemotherapy via CD8+ T cell expansion. In The Journal of clinical investigation, 130, 5461-5476. doi:10.1172/JCI136928. https://pubmed.ncbi.nlm.nih.gov/32663191/
4. Wang, Shanshan, Tian, Zhen, Lu, Yanjiao, Zhao, Jianping, Xie, Jungang. 2023. Sphingosine-1-Phosphate Receptor 4 Attenuates Neutrophilic Airway Inflammation in Experimental Asthma via Repressing Proinflammatory Macrophage Activation. In International journal of biological sciences, 19, 1597-1615. doi:10.7150/ijbs.80256. https://pubmed.ncbi.nlm.nih.gov/37056936/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen