C57BL/6JCya-Fgf2em1flox/Cya
Common Name:
Fgf2-flox
Product ID:
S-CKO-02405
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Fgf2-flox
Strain ID
CKOCMP-14173-Fgf2-B6J-VA
Gene Name
Product ID
S-CKO-02405
Gene Alias
Fgf-2; Fgf2a; Fgfb; bFGF
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fgf2em1flox/Cya mice (Catalog S-CKO-02405) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000200585
NCBI RefSeq
NM_008006
Target Region
Exon 2
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Fgf2, also known as fibroblast growth factor 2 or basic fibroblast growth factor (bFGF), is a significant protein involved in multiple biological processes. It binds with FGF receptors (FGFR) to initiate various functions. Fgf2 lacks an N-terminal secretory signal peptide and is secreted via an unconventional pathway [2]. It plays crucial roles in growth, development, and tissue repair, and is involved in pathways such as the ERK-CREB pathway [3].
In gene knockout studies, Fgf2-deficient bone marrow stromal cells (BMSCs) had higher mineralization capability and lower osteoclastogenic gene expression, and Fgf2-knockout mice showed increased bone mass, suggesting Fgf2 positively regulates osteoclastogenesis via the ERK-CREB pathway [3]. In addition, Fgf2 knockout mice had decreased alcohol consumption, failed to show alcohol-conditioned place preference, and had altered ethanol metabolism, indicating that endogenous Fgf2 is a positive regulator of alcohol-drinking behaviors [4]. In inducible, renal tubule-specific atg7 knockout (iRT-atg7-KO) mice, autophagy deficiency after acute kidney injury (AKI) suppressed FGF2 production in tubular cells, reducing fibrosis, suggesting that persistent autophagy after AKI induces FGF2 expression and secretion, which activates fibroblasts for renal fibrosis [1].
In conclusion, Fgf2 is essential in processes like bone homeostasis, alcohol-related behaviors, and renal fibrosis post-AKI. The use of Fgf2 knockout or conditional knockout mouse models has been instrumental in revealing its role in these specific disease-related areas, providing valuable insights into potential therapeutic targets for conditions such as osteoporosis, alcohol use disorder, and renal fibrosis.
References:
1. Livingston, Man J, Shu, Shaoqun, Fan, Ying, Venkatachalam, Manjeri A, Dong, Zheng. 2022. Tubular cells produce FGF2 via autophagy after acute kidney injury leading to fibroblast activation and renal fibrosis. In Autophagy, 19, 256-277. doi:10.1080/15548627.2022.2072054. https://pubmed.ncbi.nlm.nih.gov/35491858/
2. Pallotta, Maria Teresa, Nickel, Walter. 2020. FGF2 and IL-1β - explorers of unconventional secretory pathways at a glance. In Journal of cell science, 133, . doi:10.1242/jcs.250449. https://pubmed.ncbi.nlm.nih.gov/33154173/
3. Wen, Xin, Hu, Geng, Xiao, Xue, Liu, Qingxin, Li, Haifang. 2022. FGF2 positively regulates osteoclastogenesis via activating the ERK-CREB pathway. In Archives of biochemistry and biophysics, 727, 109348. doi:10.1016/j.abb.2022.109348. https://pubmed.ncbi.nlm.nih.gov/35835230/
4. Even-Chen, Oren, Herburg, Leonie, Kefalakes, Ekaterini, Grothe, Claudia, Barak, Segev. 2021. FGF2 is an endogenous regulator of alcohol reward and consumption. In Addiction biology, 27, e13115. doi:10.1111/adb.13115. https://pubmed.ncbi.nlm.nih.gov/34796591/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen