C57BL/6JCya-Got2em1flox/Cya
Common Name:
Got2-flox
Product ID:
S-CKO-02703
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Got2-flox
Strain ID
CKOCMP-14719-Got2-B6J-VA
Gene Name
Product ID
S-CKO-02703
Gene Alias
FABP-pm; Got-2; Kyat4; mAspAT
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Got2em1flox/Cya mice (Catalog S-CKO-02703) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034097
NCBI RefSeq
NM_010325
Target Region
Exon 2~3
Size of Effective Region
~2.8 kb
Detailed Document
Overview of Gene Research
GOT2, also known as glutamic-oxaloacetic transaminase 2, is a key enzyme in the malate-aspartate shuttle, which is crucial for transferring reducing equivalents from the cytosol to the mitochondria. It is involved in several critical metabolic pathways, such as glutamine metabolism, and plays an important role in maintaining intracellular NAD(H) redox balance [6]. GOT2 is also relevant in both normal cellular metabolism and dysregulated cancer metabolism [1].
In hepatocellular carcinoma (HCC), knockdown of GOT2 in cells promoted proliferation, migration, and invasion, and in mouse models, loss of GOT2 promoted tumor growth, hematogenous, and intrahepatic metastasis. Mechanistically, GOT2 silencing enhanced glutaminolysis, nucleotide synthesis, and glutathione synthesis by reprogramming glutamine metabolism, activating the PI3K/AKT/mTOR pathway [2].
In pancreatic cancer, the loss of GOT2 in cell lines in vitro disturbed redox homeostasis and halted proliferation. However, in xenograft and autochthonous mouse models, loss of GOT2 had no effect on tumor growth as the pancreatic tumor microenvironment, specifically cancer-associated fibroblasts releasing pyruvate, could rescue the proliferation of GOT2-knockdown cells [5]. Also, in pancreatic cancer, GOT2 acts as a nuclear fatty acid transporter binding to and activating the PPARδ nuclear receptor, driving immunosuppression by suppressing T cell-mediated antitumor immunity [3,4].
In conclusion, GOT2 is essential for maintaining normal metabolic and redox balance. Through gene knockout and conditional knockout mouse models, its roles in cancer, especially in HCC and pancreatic cancer, have been revealed. In HCC, it acts as a tumor-inhibitory factor, while in pancreatic cancer, it is involved in metabolic regulation and immunosuppression, providing insights into potential therapeutic targets for these diseases.
References:
1. Kerk, Samuel A, Garcia-Bermudez, Javier, Birsoy, Kivanc, Shah, Yatrik M, Lyssiotis, Costas A. 2023. Spotlight on GOT2 in Cancer Metabolism. In OncoTargets and therapy, 16, 695-702. doi:10.2147/OTT.S382161. https://pubmed.ncbi.nlm.nih.gov/37635751/
2. Li, Yunzheng, Li, Binghua, Xu, Yanchao, Sun, Beicheng, Yu, Decai. . GOT2 Silencing Promotes Reprogramming of Glutamine Metabolism and Sensitizes Hepatocellular Carcinoma to Glutaminase Inhibitors. In Cancer research, 82, 3223-3235. doi:10.1158/0008-5472.CAN-22-0042. https://pubmed.ncbi.nlm.nih.gov/35895805/
3. Nwosu, Zeribe C, Pasca di Magliano, Marina. . GOT2: An Unexpected Mediator of Immunosuppression in Pancreatic Cancer. In Cancer discovery, 12, 2237-2239. doi:10.1158/2159-8290.CD-22-0845. https://pubmed.ncbi.nlm.nih.gov/36196574/
4. Abrego, Jaime, Sanford-Crane, Hannah, Oon, Chet, Tontonoz, Peter, Sherman, Mara H. . A Cancer Cell-Intrinsic GOT2-PPARδ Axis Suppresses Antitumor Immunity. In Cancer discovery, 12, 2414-2433. doi:10.1158/2159-8290.CD-22-0661. https://pubmed.ncbi.nlm.nih.gov/35894778/
5. Kerk, Samuel A, Lin, Lin, Myers, Amy L, Shah, Yatrik M, Lyssiotis, Costas A. 2022. Metabolic requirement for GOT2 in pancreatic cancer depends on environmental context. In eLife, 11, . doi:10.7554/eLife.73245. https://pubmed.ncbi.nlm.nih.gov/35815941/
6. van Karnebeek, Clara D M, Ramos, Rúben J, Wen, Xiao-Yan, Zaki, Maha S, Wevers, Ron A. 2019. Bi-allelic GOT2 Mutations Cause a Treatable Malate-Aspartate Shuttle-Related Encephalopathy. In American journal of human genetics, 105, 534-548. doi:10.1016/j.ajhg.2019.07.015. https://pubmed.ncbi.nlm.nih.gov/31422819/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen