C57BL/6JCya-Slc3a2em1flox/Cya
Common Name:
Slc3a2-flox
Product ID:
S-CKO-03698
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Slc3a2-flox
Strain ID
CKOCMP-17254-Slc3a2-B6J-VA
Gene Name
Product ID
S-CKO-03698
Gene Alias
4F2; 4F2HC; Cd98; Ly-10; Ly-m10; Ly10; Mdu1; Mgp-2hc; NACAE
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc3a2em1flox/Cya mice (Catalog S-CKO-03698) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000170157
NCBI RefSeq
NM_001161413
Target Region
Exon 5~10
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
Slc3a2, also known as 4F2hc and CD98, is a disulfide-linked adaptor to the SLC7A5 and SLC7A11 exchangers. It is involved in the import of essential amino acids and cystine, and the export of Gln and Glu. It plays a role in oxidative metabolism-related proteostasis and is associated with pathways such as mTORC1 signaling. It is crucial for normal cellular growth and metabolism [4,5].
In lung cancer, knockdown of Slc3a2 in lung adenocarcinoma cells impaired M2 polarization of macrophages in a coculture system, suggesting it promotes tumor-associated macrophage polarization through metabolic reprogramming [1]. In ApoE-/-mice, metabolite Neu5Ac triggered Slc3a2 degradation, promoting vascular endothelial ferroptosis and aggravating atherosclerosis progression [2]. In osteoarthritis, Slc3a2 was down-regulated in OA cartilage compared to normal cartilage, and in vitro experiments validated that it inhibited ferroptosis and suppressed cartilage degeneration [3]. In laryngeal carcinoma, Slc3a2 is highly expressed, and its deficiency inhibited cell proliferation, induced ferroptosis, and suppressed tumorigenesis in nude mice via the mTOR pathway [6]. In head and neck squamous cell carcinomas, SLC3A2-deficient cells showed higher radiosensitivity and increased autophagy levels [7].
In summary, Slc3a2 plays important roles in multiple biological processes and disease conditions. Its deficiency in various KO models reveals its functions in tumor-associated macrophage polarization, endothelial ferroptosis, cartilage degeneration, tumor cell proliferation, and radiosensitivity, highlighting its potential as a therapeutic target in cancer, atherosclerosis, and osteoarthritis.
References:
1. Li, Zhuan, Chen, Songming, He, Xiang, Sun, Lunquan, Weng, Liang. 2023. SLC3A2 promotes tumor-associated macrophage polarization through metabolic reprogramming in lung cancer. In Cancer science, 114, 2306-2317. doi:10.1111/cas.15760. https://pubmed.ncbi.nlm.nih.gov/36793241/
2. Xiang, Peng, Chen, Qingqiu, Chen, Le, Yu, Chao, Ma, Limei. 2023. Metabolite Neu5Ac triggers SLC3A2 degradation promoting vascular endothelial ferroptosis and aggravates atherosclerosis progression in ApoE-/-mice. In Theranostics, 13, 4993-5016. doi:10.7150/thno.87968. https://pubmed.ncbi.nlm.nih.gov/37771765/
3. Liu, Hailong, Deng, Zengfa, Yu, Baoxi, Zeng, Anyu, Fu, Ming. 2022. Identification of SLC3A2 as a Potential Therapeutic Target of Osteoarthritis Involved in Ferroptosis by Integrating Bioinformatics, Clinical Factors and Experiments. In Cells, 11, . doi:10.3390/cells11213430. https://pubmed.ncbi.nlm.nih.gov/36359826/
4. Zhang, Cunjie, Shafaq-Zadah, Massiullah, Pawling, Judy, Johannes, Ludger, Dennis, James W. 2023. SLC3A2 N-glycosylation and Golgi remodeling regulate SLC7A amino acid exchangers and stress mitigation. In The Journal of biological chemistry, 299, 105416. doi:10.1016/j.jbc.2023.105416. https://pubmed.ncbi.nlm.nih.gov/37918808/
5. Kahlhofer, Jennifer, Teis, David. 2022. The human LAT1-4F2hc (SLC7A5-SLC3A2) transporter complex: Physiological and pathophysiological implications. In Basic & clinical pharmacology & toxicology, 133, 459-472. doi:10.1111/bcpt.13821. https://pubmed.ncbi.nlm.nih.gov/36460306/
6. Wu, Fangxing, Xiong, Gaoyun, Chen, Zejun, Liu, Qianqian, Bai, Yundan. 2022. SLC3A2 inhibits ferroptosis in laryngeal carcinoma via mTOR pathway. In Hereditas, 159, 6. doi:10.1186/s41065-022-00225-0. https://pubmed.ncbi.nlm.nih.gov/35057861/
7. Digomann, David, Linge, Annett, Dubrovska, Anna. 2019. SLC3A2/CD98hc, autophagy and tumor radioresistance: a link confirmed. In Autophagy, 15, 1850-1851. doi:10.1080/15548627.2019.1639302. https://pubmed.ncbi.nlm.nih.gov/31276435/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen