C57BL/6JCya-Myh9em1flox/Cya
Common Name:
Myh9-flox
Product ID:
S-CKO-03859
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Myh9-flox
Strain ID
CKOCMP-17886-Myh9-B6J-VA
Gene Name
Product ID
S-CKO-03859
Gene Alias
Fltn; Myhn-1; Myhn1; NMHC II-A; NMHCIIA; NMMHC-A; NMMHC-IIA; TU72.6
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Myh9em1flox/Cya mice (Catalog S-CKO-03859) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000016771
NCBI RefSeq
NM_022410
Target Region
Exon 3
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
MYH9, encoding the heavy chain of non-muscle myosin IIA, is a crucial gene. Non-muscle myosin IIA is involved in processes requiring intracellular chemomechanical force generation and actin cytoskeleton translocation. Its functions are regulated by phosphorylation of light and heavy chains and interactions with other proteins. It plays a role in embryonic development and is associated with multiple biological processes [1].
MYH9-related diseases are caused by its mutations, following an autosomal-dominant pattern. These diseases include macrothrombocytopenia, leukocyte inclusions, and risks of renal failure, hearing loss, and cataracts [2]. In tumors, MYH9 has dual functions. In hepatocellular carcinoma, it promotes tumor stemness by interacting with GSK3β and reducing its protein expression, and HBX can induce MYH9 expression [3]. In gastric cancer, nuclear MYH9 promotes metastasis by inducing CTNNB1 transcription [4]. In rheumatoid arthritis, MYH9 promotes synoviocyte migration and invasion [5].
In conclusion, MYH9 is essential for generating intracellular force and actin-related processes. Its mutations lead to MYH9-related diseases. In addition, MYH9 is closely associated with tumor progression and some autoimmune diseases. Studies on MYH9-related KO or CKO mouse models (not specifically mentioned in provided references but valuable in general gene-function studies) could further clarify its role in these disease conditions, providing potential therapeutic targets for relevant diseases.
References:
1. Pecci, Alessandro, Ma, Xuefei, Savoia, Anna, Adelstein, Robert S. 2018. MYH9: Structure, functions and role of non-muscle myosin IIA in human disease. In Gene, 664, 152-167. doi:10.1016/j.gene.2018.04.048. https://pubmed.ncbi.nlm.nih.gov/29679756/
2. Furlano, Mónica, Arlandis, Rosa, Venegas, María Del Prado, Ars, Elisabet, Torra, Roser. 2018. MYH9 Associated nephropathy. In Nefrologia, 39, 133-140. doi:10.1016/j.nefro.2018.08.008. https://pubmed.ncbi.nlm.nih.gov/30471777/
3. Lin, Xian, Li, Ai-Min, Li, Yong-Hao, Liu, Zhen, Fang, Wei-Yi. 2020. Silencing MYH9 blocks HBx-induced GSK3β ubiquitination and degradation to inhibit tumor stemness in hepatocellular carcinoma. In Signal transduction and targeted therapy, 5, 13. doi:10.1038/s41392-020-0111-4. https://pubmed.ncbi.nlm.nih.gov/32296025/
4. Ye, Gengtai, Yang, Qingbin, Lei, Xuetao, Yu, Jiang, Li, Guoxin. 2020. Nuclear MYH9-induced CTNNB1 transcription, targeted by staurosporin, promotes gastric cancer cell anoikis resistance and metastasis. In Theranostics, 10, 7545-7560. doi:10.7150/thno.46001. https://pubmed.ncbi.nlm.nih.gov/32685004/
5. Lee, Saseong, Choi, Eunbyeol, Chae, Sehyun, Hwang, Daehee, Kim, Wan-Uk. 2023. Identification of MYH9 as a key regulator for synoviocyte migration and invasion through secretome profiling. In Annals of the rheumatic diseases, 82, 1035-1048. doi:10.1136/ard-2022-223625. https://pubmed.ncbi.nlm.nih.gov/37188496/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen