C57BL/6JCya-Nat1em1flox/Cya
Common Name:
Nat1-flox
Product ID:
S-CKO-03893
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Nat1-flox
Strain ID
CKOCMP-17960-Nat1-B6J-VA
Gene Name
Product ID
S-CKO-03893
Gene Alias
Nat-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nat1em1flox/Cya mice (Catalog S-CKO-03893) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000026677
NCBI RefSeq
NM_008673
Target Region
Exon 1
Size of Effective Region
~2.9 kb
Detailed Document
Overview of Gene Research
NAT1, also known as N-acetyltransferase 1, is a polymorphic Phase II enzyme. It plays a crucial role in metabolizing heterocyclic and aromatic amines [1,4,5]. These metabolic processes are associated with various biological pathways related to carcinogen biotransformation. NAT1's function is of great biological importance as it can influence cancer susceptibility and other disease risks [1,4,5]. Genetic models, such as gene knockout models, can be valuable for studying NAT1.
In colorectal cancer, NAT1 overexpression inhibits the PI3K/AKT/mTOR signaling pathway, suppressing the epithelial-mesenchymal transition (EMT) process, glycolytic ability, and VEGF expression, ultimately reducing liver metastasis [2]. In breast cancer cells, NAT1 deletion leads to upregulation of cytidine deaminase, which is involved in the pyrimidine salvage pathway [3]. In mice, Nat1 deficiency (mouse ortholog of human NAT1) results in mitochondrial dysfunction, characterized by increased reactive oxygen species, mitochondrial fragmentation, and decreased mitochondrial functions, leading to exercise intolerance [7].
In conclusion, NAT1 is essential in processes like carcinogen metabolism and has a significant impact on diseases such as colorectal cancer, breast cancer, and potentially asthma in children with secondhand smoke exposure [6]. Mouse models, especially those with Nat1 deficiency, have revealed its role in mitochondrial function and provided insights into its contribution to disease-related biological processes.
References:
1. Dhaini, Hassan R, El Hafi, Bassam, Khamis, Assem M. 2017. NAT1 genotypic and phenotypic contribution to urinary bladder cancer risk: a systematic review and meta-analysis. In Drug metabolism reviews, 50, 208-219. doi:10.1080/03602532.2017.1415928. https://pubmed.ncbi.nlm.nih.gov/29258340/
2. Gu, Wang, Li, Chen, Shen, Tingting, Zhang, Chong, Zhang, Chao. 2024. NAT1 inhibits liver metastasis of colorectal cancer by regulating EMT and glycolysis. In Aging, 16, 10546-10562. doi:10.18632/aging.205957. https://pubmed.ncbi.nlm.nih.gov/38916406/
3. Hong, Kyung U, Tagnedji, Afi H, Doll, Mark A, Walls, Kennedy M, Hein, David W. 2022. Upregulation of cytidine deaminase in NAT1 knockout breast cancer cells. In Journal of cancer research and clinical oncology, 149, 5047-5060. doi:10.1007/s00432-022-04436-w. https://pubmed.ncbi.nlm.nih.gov/36329350/
4. Hein, D W, Doll, M A, Fretland, A J, Nangju, N A, Feng, Y. . Molecular genetics and epidemiology of the NAT1 and NAT2 acetylation polymorphisms. In Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 9, 29-42. doi:. https://pubmed.ncbi.nlm.nih.gov/10667461/
5. Hein, David W. . Molecular genetics and function of NAT1 and NAT2: role in aromatic amine metabolism and carcinogenesis. In Mutation research, 506-507, 65-77. doi:. https://pubmed.ncbi.nlm.nih.gov/12351146/
6. Brooks, Cassandra C, Martin, Lisa J, Pilipenko, Valentina, Khurana Hershey, Gurjit K, Biagini Myers, Jocelyn M. 2019. NAT1 genetic variation increases asthma risk in children with secondhand smoke exposure. In The Journal of asthma : official journal of the Association for the Care of Asthma, 58, 284-292. doi:10.1080/02770903.2019.1694941. https://pubmed.ncbi.nlm.nih.gov/31809667/
7. Chennamsetty, Indumathi, Coronado, Michael, Contrepois, Kévin, Quertermous, Thomas, Knowles, Joshua W. . Nat1 Deficiency Is Associated with Mitochondrial Dysfunction and Exercise Intolerance in Mice. In Cell reports, 17, 527-540. doi:10.1016/j.celrep.2016.09.005. https://pubmed.ncbi.nlm.nih.gov/27705799/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen