C57BL/6JCya-Per1em1flox/Cya
Common Name:
Per1-flox
Product ID:
S-CKO-04240
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Per1-flox
Strain ID
CKOCMP-18626-Per1-B6J-VA
Gene Name
Product ID
S-CKO-04240
Gene Alias
Hftm; Per; m-rigui; mPer1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Per1em1flox/Cya mice (Catalog S-CKO-04240) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000021271
NCBI RefSeq
NM_011065
Target Region
Exon 2~8
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Per1, also known as Period circadian regulator 1, is a core component of the circadian clock [4,5,6,7,8,9,10]. It functions with other clock components to generate a feedback loop involving the transcriptional repression of gene expression, producing a circadian rhythm with an approximately 24-hour cycle [9]. PER1 is involved in regulating various biological processes, such as metabolism, immune response, and cell cycle regulation, and is associated with multiple pathways [1,2,3,5,6,7,8,10].
In trastuzumab-resistant HER2-positive gastric cancer cells, PER1, along with PPARγ, regulates the circadian oscillation of HK2-dependent glycolysis, and silencing PER1 disrupts this rhythm and reverses trastuzumab resistance [1]. In pancreatic cancer, the m6A demethylase ALKBH5 post-transcriptionally activates PER1, which in turn re-activates the ATM-CHK2-P53/CDC25C signalling to inhibit cell growth [2]. In ocular melanoma, histone lactylation promotes YTHDF2 expression, and YTHDF2 recognizes m6A-modified PER1 mRNA and promotes its degradation, accelerating tumorigenesis [3]. In mice, Per1 deficiency deregulates daily oxygen consumption rhythm, mitochondrial dynamics, and cellular GPx-related ROS fluctuations [6]. In rat mandibular condylar chondrocytes, PER1 can regulate the expression of MMP13 through the NF-κB pathway in IL-1β-induced cells [7]. In hindlimb ischemia (HLI) mouse models, PER1 overexpression promotes functional recovery by reducing M1 macrophage polarization and promoting M2 macrophage polarization [8]. In rats, WNK3 can phosphorylate PER1 to promote its degradation and is associated with circadian oscillations [9]. In ovarian cancer, PER1 expression is lower in tumor tissues, and low PER1 expression is related to a reduced overall survival rate [10].
In conclusion, Per1 is crucial for maintaining the circadian rhythm and is involved in various biological processes and diseases. Studies using gene-knockout or conditional-knockout mouse models and other loss-of-function experiments have revealed its significant role in cancer, metabolic diseases, and inflammatory conditions, providing valuable insights into potential therapeutic targets and disease mechanisms [1,2,3,5,6,7,8,9,10].
References:
1. Wang, Jiao, Huang, Qiong, Hu, Xingbin, Liao, Wangjun, Shi, Min. . Disrupting Circadian Rhythm via the PER1-HK2 Axis Reverses Trastuzumab Resistance in Gastric Cancer. In Cancer research, 82, 1503-1517. doi:10.1158/0008-5472.CAN-21-1820. https://pubmed.ncbi.nlm.nih.gov/35255118/
2. Guo, Xingya, Li, Kai, Jiang, Weiliang, Pan, Qin, Wan, Rong. 2020. RNA demethylase ALKBH5 prevents pancreatic cancer progression by posttranscriptional activation of PER1 in an m6A-YTHDF2-dependent manner. In Molecular cancer, 19, 91. doi:10.1186/s12943-020-01158-w. https://pubmed.ncbi.nlm.nih.gov/32429928/
3. Yu, Jie, Chai, Peiwei, Xie, Minyue, Fan, Xianqun, Jia, Renbing. 2021. Histone lactylation drives oncogenesis by facilitating m6A reader protein YTHDF2 expression in ocular melanoma. In Genome biology, 22, 85. doi:10.1186/s13059-021-02308-z. https://pubmed.ncbi.nlm.nih.gov/33726814/
4. Milićević, Nemanja, Bergen, Arthur A, Felder-Schmittbuhl, Marie-Paule. 2022. Per1 mutation enhances masking responses in mice. In Chronobiology international, 39, 1533-1538. doi:10.1080/07420528.2022.2126321. https://pubmed.ncbi.nlm.nih.gov/36189750/
5. Chen, Yuanyuan, Liu, Zhaohua, Chen, Hongmei, Fan, Lang, Luo, Man. 2024. Rhythm gene PER1 mediates ferroptosis and lipid metabolism through SREBF2/ALOX15 axis in polycystic ovary syndrome. In Biochimica et biophysica acta. Molecular basis of disease, 1870, 167182. doi:10.1016/j.bbadis.2024.167182. https://pubmed.ncbi.nlm.nih.gov/38653359/
6. Sun, Qi, Yang, Yunxia, Wang, Zhongqiu, Wang, Junsong, Zhang, Jianfa. 2020. PER1 interaction with GPX1 regulates metabolic homeostasis under oxidative stress. In Redox biology, 37, 101694. doi:10.1016/j.redox.2020.101694. https://pubmed.ncbi.nlm.nih.gov/32896721/
7. Wei, Jia-Ming, Tu, Shao-Qin, Wang, Yu-Xuan, Ai, Hong, Chen, Zheng. 2023. Clock gene Per1 regulates rat temporomandibular osteoarthritis through NF-κB pathway: an in vitro and in vivo study. In Journal of orthopaedic surgery and research, 18, 817. doi:10.1186/s13018-023-04301-7. https://pubmed.ncbi.nlm.nih.gov/37907921/
8. Ding, Yang, Wan, Shengyun, Ma, Long, Wei, Kaikai, Ye, Kun. 2023. PER1 promotes functional recovery of mice with hindlimb ischemia by inducing anti-inflammatory macrophage polarization. In Biochemical and biophysical research communications, 644, 62-69. doi:10.1016/j.bbrc.2023.01.001. https://pubmed.ncbi.nlm.nih.gov/36634583/
9. Zhang, Zhao-Huan, Xiong, Jian-Mei, Zhu, Yun-Yi, Zhuang, Jian-Hua, Xu, Xiao-Hui. 2022. WNK3-PER1 interactions regulate the circadian rhythm in the suprachiasmatic nucleus in rats. In American journal of translational research, 14, 1001-1009. doi:. https://pubmed.ncbi.nlm.nih.gov/35273702/
10. Chen, Mali, Zhang, Lili, Liu, Xiaolong, Ma, Zhen, Lv, Ling. 2021. PER1 Is a Prognostic Biomarker and Correlated With Immune Infiltrates in Ovarian Cancer. In Frontiers in genetics, 12, 697471. doi:10.3389/fgene.2021.697471. https://pubmed.ncbi.nlm.nih.gov/34220965/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen