C57BL/6JCya-Soat1em1flox/Cya
Common Name:
Soat1-flox
Product ID:
S-CKO-05167
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Soat1-flox
Strain ID
CKOCMP-20652-Soat1-B6J-VA
Gene Name
Product ID
S-CKO-05167
Gene Alias
8430426K15Rik; ACAT-1; Acact; ald; hid
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Soat1em1flox/Cya mice (Catalog S-CKO-05167) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000189661
NCBI RefSeq
NM_009230
Target Region
Exon 3
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
SOAT1, also known as acyl-coenzyme A (CoA):cholesterol acyltransferase 1 (ACAT1), is a key enzyme in lipid metabolism. It localizes in the endoplasmic reticulum and mediates cholesterol esterification, converting cholesterol to cholesterol esters for storage in lipid droplets. This process is crucial for maintaining cholesterol homeostasis, which is involved in multiple biological pathways such as the mevalonate pathway [2,4]. Cholesterol homeostasis is vital for various cellular functions, including adipocyte function and cell membrane integrity [4]. Genetic models, like gene-knockout (KO) mouse models, have been valuable for studying SOAT1's functions.
In pancreatic cancer, genetic targeting of Soat1 in mouse models impairs cell proliferation in vitro and tumor progression in vivo, revealing a mevalonate pathway dependency in p53 mutant PDAC cells with p53 loss of heterozygosity (LOH) [2]. In hepatocellular carcinoma, SOAT1 promotes epithelial-mesenchymal transition (EMT) and tumorigenesis. Nootkatone, which targets SOAT1, can inhibit EMT in vitro and in vivo [1]. In colorectal cancer, silencing SOAT1 in cell lines strongly inhibits the migration and invasion ability of CRC tumor cells, indicating its role in promoting cancer progression [3]. In gastric cancer, knockdown of SOAT1 or its pharmacological inhibition suppresses cell proliferation, cholesterol ester synthesis, and lymphangiogenesis, while overexpression promotes these processes [5].
In conclusion, SOAT1 plays essential roles in lipid metabolism, especially in maintaining cholesterol homeostasis. Through KO or related mouse models, it has been shown to significantly influence the development and progression of multiple cancers, including pancreatic, hepatocellular, colorectal, and gastric cancers. Understanding SOAT1's functions in these disease contexts provides potential therapeutic targets for cancer treatment.
References:
1. Fu, Rongrong, Xue, Wenqing, Liang, Jingjie, Zhang, Min, Meng, Jing. 2024. SOAT1 regulates cholesterol metabolism to induce EMT in hepatocellular carcinoma. In Cell death & disease, 15, 325. doi:10.1038/s41419-024-06711-9. https://pubmed.ncbi.nlm.nih.gov/38724499/
2. Oni, Tobiloba E, Biffi, Giulia, Baker, Lindsey A, Vakoc, Christopher R, Tuveson, David A. . SOAT1 promotes mevalonate pathway dependency in pancreatic cancer. In The Journal of experimental medicine, 217, . doi:10.1084/jem.20192389. https://pubmed.ncbi.nlm.nih.gov/32633781/
3. Wang, Xin-Chun, Luo, Lin-Ming, Huang, Tao-Sheng, Feng, Li-Feng. 2021. SOAT1 is a new prognostic factor of colorectal cancer. In Irish journal of medical science, 191, 1549-1554. doi:10.1007/s11845-021-02746-5. https://pubmed.ncbi.nlm.nih.gov/34460058/
4. Liu, Qing, Wu, Xiaolin, Duan, Wei, Zhou, Zhangsen, Zhu, Yuyan. 2024. ACAT1/SOAT1 maintains adipogenic ability in preadipocytes by regulating cholesterol homeostasis. In Journal of lipid research, 65, 100680. doi:10.1016/j.jlr.2024.100680. https://pubmed.ncbi.nlm.nih.gov/39481851/
5. Zhu, Tingting, Wang, Zhangding, Zou, Tianhui, Ding, Qingqing, Xu, Guifang. 2021. SOAT1 Promotes Gastric Cancer Lymph Node Metastasis Through Lipid Synthesis. In Frontiers in pharmacology, 12, 769647. doi:10.3389/fphar.2021.769647. https://pubmed.ncbi.nlm.nih.gov/34790132/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen