C57BL/6JCya-Lacc1em1flox/Cya
Common Name:
Lacc1-flox
Product ID:
S-CKO-05551
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Lacc1-flox
Strain ID
CKOCMP-210808-Lacc1-B6J-VA
Gene Name
Product ID
S-CKO-05551
Gene Alias
9030625A04Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lacc1em1flox/Cya mice (Catalog S-CKO-05551) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000062789
NCBI RefSeq
NM_172488
Target Region
Exon 4
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Lacc1, also known as C13orf31, is an enzyme highly expressed in inflammatory macrophages. It plays a central regulatory role in multiple inflammatory diseases such as inflammatory bowel diseases, arthritis, and microbial infections [1,2,3,4,5,6,7,8]. Biochemically, Lacc1 converts L-citrulline to L-ornithine and isocyanic acid, bridging proinflammatory nitric oxide synthase (NOS2) and polyamine immunometabolism [1,2].
In Lacc1 -/- mice, more severe colon lesions were observed after oral administration of Citrobacter rodentium, and an accelerated onset of arthritis and more severe inflammation occurred in collagen-induced arthritis and mannan-induced arthritis models, with increased serum and local TNF and elevated percentage of IL-17A-producing CD4+ T cells [6]. Lacc1 -/- mice also had more severe T-cell transfer colitis, increased bacterial burden in intestinal lymphoid organs, and altered T-cell cytokine levels [7]. Additionally, Lacc1 deletion aggravated dextran sodium sulfate (DSS)-induced inflammatory bowel disease in mice, with significant changes in the intestinal flora [8]. In human immune cells, LACC1 deficiency was associated with a novel form of genetically inherited juvenile arthritis linked to impaired autophagy in macrophages [3].
In conclusion, Lacc1 is crucial in regulating inflammation, bacterial clearance, and autophagy in macrophages. Gene knockout mouse models have revealed its important role in inflammatory bowel disease, arthritis, and other inflammatory diseases, providing insights into the mechanisms of these diseases and potential therapeutic targets.
References:
1. Wei, Zheng, Oh, Joonseok, Flavell, Richard A, Crawford, Jason M. 2022. LACC1 bridges NOS2 and polyamine metabolism in inflammatory macrophages. In Nature, 609, 348-353. doi:10.1038/s41586-022-05111-3. https://pubmed.ncbi.nlm.nih.gov/35978195/
2. Li, Yaling, Wu, Zhixiong, Tan, Xiaoli, Tang, Liang, Ouyang, Fan. 2023. LACC1: A critical involvement in macrophage immunometabolism. In Cell biology international, 47, 1488-1490. doi:10.1002/cbin.12063. https://pubmed.ncbi.nlm.nih.gov/37366569/
3. Omarjee, Ommar, Mathieu, Anne-Laure, Quiniou, Gaëlle, Walzer, Thierry, Belot, Alexandre. . LACC1 deficiency links juvenile arthritis with autophagy and metabolism in macrophages. In The Journal of experimental medicine, 218, . doi:10.1084/jem.20201006. https://pubmed.ncbi.nlm.nih.gov/33606008/
4. Xiong, Yulong, Zhang, Zhenhao, Liu, Shangyu, Zhou, Likun, Yao, Yan. 2023. Lupeol alleviates autoimmune myocarditis by suppressing macrophage pyroptosis and polarization via PPARα/LACC1/NF-κB signaling pathway. In Phytomedicine : international journal of phytotherapy and phytopharmacology, 123, 155193. doi:10.1016/j.phymed.2023.155193. https://pubmed.ncbi.nlm.nih.gov/37976692/
5. He, Tingyan, Wang, Linlin, Huang, Xiaomei, Weng, Ruohang, Yang, Jun. 2024. LACC1 deficiency leading to juvenile arthritis and anemia. In Clinical immunology (Orlando, Fla.), 265, 110290. doi:10.1016/j.clim.2024.110290. https://pubmed.ncbi.nlm.nih.gov/38944365/
6. Skon-Hegg, Cara, Zhang, Juan, Wu, Xiumin, Lee, Wyne P, Behrens, Timothy W. 2018. LACC1 Regulates TNF and IL-17 in Mouse Models of Arthritis and Inflammation. In Journal of immunology (Baltimore, Md. : 1950), 202, 183-193. doi:10.4049/jimmunol.1800636. https://pubmed.ncbi.nlm.nih.gov/30510070/
7. Kang, Jung-Woo, Yan, Jie, Ranjan, Kishu, Turner, Jerrold R, Abraham, Clara. 2020. Myeloid Cell Expression of LACC1 Is Required for Bacterial Clearance and Control of Intestinal Inflammation. In Gastroenterology, 159, 1051-1067. doi:10.1053/j.gastro.2020.07.024. https://pubmed.ncbi.nlm.nih.gov/32693188/
8. Xu, Zheng-Yuan, Wang, Jin-Chun. 2023. LACC1 regulates changes in the intestinal flora in a mouse model of inflammatory bowel disease. In BMC gastroenterology, 23, 358. doi:10.1186/s12876-023-02971-5. https://pubmed.ncbi.nlm.nih.gov/37848840/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen