C57BL/6JCya-Tyrobpem1flox/Cya
Common Name:
Tyrobp-flox
Product ID:
S-CKO-06504
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Tyrobp-flox
Strain ID
CKOCMP-22177-Tyrobp-B6J-VA
Gene Name
Product ID
S-CKO-06504
Gene Alias
DAP12; KARAP; Ly83
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tyrobpem1flox/Cya mice (Catalog S-CKO-06504) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000032800
NCBI RefSeq
NM_011662
Target Region
Exon 2~4
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
TYROBP, also known as DAP12 or KARAP, is a transmembrane adaptor protein. It functions as a receptor-activating subunit in natural killer (NK) cells and is expressed in multiple cell types like monocytes, macrophages, dendritic cells, osteoclasts, and microglia. TYROBP acts as a downstream adaptor and signaling partner for several receptors, such as SIRP1β, CD33, CR3, and TREM2, which are involved in signal transduction, especially in maintaining brain homeostasis [1,2].
In Alzheimer's disease (AD) mouse models, TYROBP-deficient mice showed genetic resilience to amyloidosis and tauopathy. Learning and synaptic functions were preserved, and complement C1q accumulation was prevented [1]. In Huntington's disease (HD) Q175 mouse models, Tyrobp knockout decreased microglial expression of disease-associated genes, mitigated astrogliosis, and improved motor function [4]. In aortic dissection (AD) mouse models, global or myeloid cell-specific deficiency of Tyrobp increased AD incidence, while enhancement of Trem2/Tyrobp signaling protected against AD [3].
In conclusion, TYROBP is crucial for signal transduction across multiple receptors in various cell types. Studies using KO mouse models have revealed its significant roles in neurodegenerative diseases like Alzheimer's and Huntington's, as well as in cardiovascular conditions such as aortic dissection. These models help understand the gene's function in disease-related biological processes, providing potential therapeutic targets.
References:
1. Haure-Mirande, Jean-Vianney, Audrain, Mickael, Ehrlich, Michelle E, Gandy, Sam. 2022. Microglial TYROBP/DAP12 in Alzheimer's disease: Transduction of physiological and pathological signals across TREM2. In Molecular neurodegeneration, 17, 55. doi:10.1186/s13024-022-00552-w. https://pubmed.ncbi.nlm.nih.gov/36002854/
2. Ma, Jing, Jiang, Teng, Tan, Lan, Yu, Jin-Tai. 2014. TYROBP in Alzheimer's disease. In Molecular neurobiology, 51, 820-6. doi:10.1007/s12035-014-8811-9. https://pubmed.ncbi.nlm.nih.gov/25052481/
3. Zhang, Zenghui, Wu, Maoxiong, Yao, Lei, Liu, Zhaoyu, Chen, Yangxin. 2024. Trem2/Tyrobp Signaling Protects Against Aortic Dissection and Rupture by Inhibiting Macrophage Activation in Mice. In Arteriosclerosis, thrombosis, and vascular biology, 45, 119-135. doi:10.1161/ATVBAHA.124.321429. https://pubmed.ncbi.nlm.nih.gov/39508103/
4. Creus-Muncunill, Jordi, Haure-Mirande, Jean Vianney, Mattei, Daniele, Ellerby, Lisa M, Ehrlich, Michelle E. 2024. TYROBP/DAP12 knockout in Huntington's disease Q175 mice cell-autonomously decreases microglial expression of disease-associated genes and non-cell-autonomously mitigates astrogliosis and motor deterioration. In Journal of neuroinflammation, 21, 66. doi:10.1186/s12974-024-03052-4. https://pubmed.ncbi.nlm.nih.gov/38459557/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen