C57BL/6JCya-Fmnl3em1flox/Cya
Common Name:
Fmnl3-flox
Product ID:
S-CKO-06664
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Fmnl3-flox
Strain ID
CKOCMP-22379-Fmnl3-B6J-VA
Gene Name
Product ID
S-CKO-06664
Gene Alias
2700073B04Rik; FBP11; Wbp3; mKIAA2014
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fmnl3em1flox/Cya mice (Catalog S-CKO-06664) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000088233
NCBI RefSeq
NM_011711
Target Region
Exon 3~4
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Fmnl3, or Formin-like 3, is a member of the formin-likes within the formin family. As an F-actin nucleator, it is crucial for functions such as polarity control, invasion, and migration [1]. It is involved in pathways related to cell-cell adhesion, cytoskeletal mediation, and may be associated with tumorigenesis pathways [1,2,3].
In mouse oocytes, FMNL3 depletion led to polar body extrusion failure and symmetric meiotic division, with spindle migration defects at late metaphase I likely due to decreased cytoplasmic actin. FMNL3 interacts with the actin-binding protein FASCIN to regulate actin filaments during oocyte meiosis [1]. In cancer research, FMNL3 is overexpressed in pancreatic cancer, nasopharyngeal carcinoma, tongue squamous cell carcinoma, and breast cancer, promoting cell metastasis, invasion, and associated with poor prognosis. For example, in nasopharyngeal carcinoma, TGF-β1/FMNL3 signalling may mediate epithelial-to-mesenchymal transition (EMT) related to metastasis [1,2,4,5]. In melanoma, inhibition of FMNL3 affected cell morphology and migration [6].
In summary, Fmnl3 is essential for actin-mediated processes like spindle migration and cytokinesis in oocytes. In disease, especially cancer, Fmnl3 is often overexpressed, promoting metastasis and invasion. Studies using gene knockout or conditional knockout models in mice and cell-based loss-of-function experiments have been crucial in revealing these functions, helping understand the biological mechanisms underlying oocyte development and cancer progression.
References:
1. Pan, Meng-Hao, Wan, Xiang, Wang, Hong-Hui, Zhang, Yu, Sun, Shao-Chen. . FMNL3 regulates FASCIN for actin-mediated spindle migration and cytokinesis in mouse oocytes†. In Biology of reproduction, 102, 1203-1212. doi:10.1093/biolre/ioaa033. https://pubmed.ncbi.nlm.nih.gov/32167535/
2. Wu, Yanxia, Shen, Zhihua, Wang, Keke, Jiang, Hanguo, Jie, Wei. 2017. High FMNL3 expression promotes nasopharyngeal carcinoma cell metastasis: role in TGF-β1-induced epithelia-to-mesenchymal transition. In Scientific reports, 7, 42507. doi:10.1038/srep42507. https://pubmed.ncbi.nlm.nih.gov/28198387/
3. Gauvin, Timothy J, Young, Lorna E, Higgs, Henry N. 2014. The formin FMNL3 assembles plasma membrane protrusions that participate in cell-cell adhesion. In Molecular biology of the cell, 26, 467-77. doi:10.1091/mbc.E14-07-1247. https://pubmed.ncbi.nlm.nih.gov/25428984/
4. Liu, Jiameng, Chen, Shan, Chen, Yang, Geng, Ningbo, Feng, Chongjin. 2019. High expression of FMNL3 associates with cancer cell migration, invasion, and unfavorable prognosis in tongue squamous cell carcinoma. In Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, 48, 459-467. doi:10.1111/jop.12857. https://pubmed.ncbi.nlm.nih.gov/30955218/
5. Zhao, Binggong, Ye, Dong-Man, Li, Shujing, Yu, Tao, Wu, Huijian. 2024. FMNL3 Promotes Migration and Invasion of Breast Cancer Cells via Inhibiting Rad23B-Induced Ubiquitination of Twist1. In Journal of cellular physiology, 240, e31481. doi:10.1002/jcp.31481. https://pubmed.ncbi.nlm.nih.gov/39582466/
6. Gardberg, Maria, Heuser, Vanina D, Koskivuo, Ilkka, Koivisto, Mari, Carpén, Olli. 2016. FMNL2/FMNL3 formins are linked with oncogenic pathways and predict melanoma outcome. In The journal of pathology. Clinical research, 2, 41-52. doi:10.1002/cjp2.34. https://pubmed.ncbi.nlm.nih.gov/27499915/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen