C57BL/6JCya-Clca2em1flox/Cya
Common Name
Clca2-flox
Product ID
S-CKO-07302
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-229933-Clca2-B6J-VA
Status
When using this mouse strain in a publication, please cite “Clca2-flox Mouse (Catalog S-CKO-07302) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Clca2-flox
Strain ID
CKOCMP-229933-Clca2-B6J-VA
Gene Name
Product ID
S-CKO-07302
Gene Alias
Clca5, 4732440A06
Background
C57BL/6JCya
Gene Full Name
chloride channel accessory 2
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 3 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000040465
NCBI Transcript ID
NM_178697
Target Region
Exon 3
Size of Effective Region
~1.7 kb
Overview of Gene Research
Clca2, short for Calcium-activated chloride channel A2, is a transmembrane protein with multiple functions. It is involved in regulating calcium-activated chloride channel (CaCC) currents, and has been associated with pathways like Wnt/β-catenin, ERK/JNK/p38-MAPK, and is regulated by p53 family proteins. It plays a crucial role in processes such as cell proliferation, migration, invasion, and epithelial-to-mesenchymal transition (EMT), and is important in maintaining cellular homeostasis and preventing premature senescence [1-10].
In cervical cancer, knockdown of Clca2 by small interfering RNA suppressed tumor cell proliferation and migration, and Clca2 was found to be involved in the Wnt/β-catenin signaling pathway. Overexpression of Clca2 inhibited the proliferation, migration, invasion, and promoted apoptosis of cervical cancer cells, and it inhibited EMT through the p38 / JNK / ERK pathway [1,2]. In triple-negative breast cancer, high Clca2 expression was associated with a higher risk of death among African American women [6]. In breast cancer, the absence of Clca2 expression in several tumours and cell lines was due to promoter region hypermethylation, and overexpressing Clca2 in negative cell lines reduced their tumorigenicity and metastasis capability [7]. Depletion of Clca2 led to accelerated senescence onset in UVB and Nutlin3a-induced senescence models, and in 3D skin equivalent models, Clca2 knockdown fibroblasts exhibited aged-like skin features [3]. In keratinocytes, Clca2 is transported to the nucleus via extracellular vesicles, where it binds to and activates β-catenin, affecting cell survival and migration [4]. In human mammary epithelial cells, knockdown of Clca2 caused EMT, and it was found to interact with EVA1, which is essential for epithelial differentiation [5].
In conclusion, Clca2 is a key regulator in multiple biological processes. Through various gene-based functional studies including loss-of-function experiments, its role in cancer development, cellular senescence, and skin homeostasis has been revealed. These findings suggest Clca2 as a potential therapeutic target for cancers like cervical and breast cancer, and further understanding of its functions could provide new insights into disease mechanisms and treatment strategies.
References：
1. Zhang, Peijin, Lin, Yang, Liu, Yaqiong. 2021. CLCA2 suppresses the proliferation, migration and invasion of cervical cancer. In Experimental and therapeutic medicine, 22, 776. doi:10.3892/etm.2021.10208. https://pubmed.ncbi.nlm.nih.gov/34055075/
2. Xin, Wenhu, Zhang, Jian, Zhang, Haibin, Li, Yufeng, Wang, Fang. 2022. CLCA2 overexpression suppresses epithelial-to-mesenchymal transition in cervical cancer cells through inactivation of ERK/JNK/p38-MAPK signaling pathways. In BMC molecular and cell biology, 23, 44. doi:10.1186/s12860-022-00440-7. https://pubmed.ncbi.nlm.nih.gov/36280802/
3. Guerrero-Navarro, Lena, Martic, Ines, Ploner, Christian, Jansen-Dürr, Pidder, Cavinato, Maria. 2024. CLCA2: A Potential Guardian against Premature Senescence and Skin Aging. In Biomedicines, 12, . doi:10.3390/biomedicines12030592. https://pubmed.ncbi.nlm.nih.gov/38540205/
4. Seltmann, Kristin, Hettich, Britta, Abele, Seraina, Leroux, Jean-Christophe, Werner, Sabine. . Transport of CLCA2 to the nucleus by extracellular vesicles controls keratinocyte survival and migration. In Journal of extracellular vesicles, 13, e12430. doi:10.1002/jev2.12430. https://pubmed.ncbi.nlm.nih.gov/38602325/
5. Ramena, Grace, Yin, Yufang, Yu, Yang, Walia, Vijay, Elble, Randolph C. 2016. CLCA2 Interactor EVA1 Is Required for Mammary Epithelial Cell Differentiation. In PloS one, 11, e0147489. doi:10.1371/journal.pone.0147489. https://pubmed.ncbi.nlm.nih.gov/26930581/
6. Purrington, Kristen S, Knight, Jimmie, Dyson, Gregory, Boerner, Julie L, Bandyopadhyay, Sudeshna. 2020. CLCA2 expression is associated with survival among African American women with triple negative breast cancer. In PloS one, 15, e0231712. doi:10.1371/journal.pone.0231712. https://pubmed.ncbi.nlm.nih.gov/32298355/
7. Li, Xiurong, Cowell, John K, Sossey-Alaoui, Khalid. . CLCA2 tumour suppressor gene in 1p31 is epigenetically regulated in breast cancer. In Oncogene, 23, 1474-80. doi:. https://pubmed.ncbi.nlm.nih.gov/14973555/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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