C57BL/6JCya-Tmem41bem1flox/Cya
Common Name:
Tmem41b-flox
Product ID:
S-CKO-07664
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tmem41b-flox
Strain ID
CKOCMP-233724-Tmem41b-B6J-VA
Gene Name
Product ID
S-CKO-07664
Gene Alias
1500015G02Rik; 1500031M19Rik; D7Ertd70e; D7Ertd743e
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tmem41bem1flox/Cya mice (Catalog S-CKO-07664) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000094097
NCBI RefSeq
NM_153525
Target Region
Exon 3~5
Size of Effective Region
~3.2 kb
Detailed Document
Overview of Gene Research
TMEM41B, also known as Stasimon, is an endoplasmic reticulum (ER)-localized transmembrane protein with phospholipid scrambling activity. It is involved in multiple essential biological processes, including lipoprotein biogenesis, lipid homeostasis, autophagosome formation, and plays a significant role in various viral infections. It has been associated with pathways related to lipid metabolism and autophagy, and its function is crucial for maintaining cellular and organismal fitness [2,4,5,6,7,8,9]. Genetic models, such as KO and CKO mouse models, have been valuable in studying its functions.
Full-genome loss-of-function CRISPR-Cas9 screens identified TMEM41B as a pan-flavivirus host factor. It is required for the infection of all tested Flaviviridae family members and SARS-CoV-2 of the Coronaviridae family. Single nucleotide polymorphisms present in nearly 20% of East Asian populations reduce flavivirus infection. Mechanistically, TMEM41B is recruited to flavivirus RNA replication complexes to facilitate membrane curvature for viral genome replication [1]. In β -coronavirus infection, TMEM41B is essential for double-membrane vesicle (DMV) formation. In TMEM41B KO cells, the interaction between viral non-structural proteins nsp3 and nsp4 is reduced, suppressing DMV formation [3]. Hepatic TMEM41B loss eliminates plasma lipids due to the absence of mature lipoproteins in the ER but paradoxically activates lipid production, leading to non-alcoholic hepatosteatosis in TMEM41B -deficient mice [2]. Also, TMEM41B knockdown suppresses Pseudorabies virus (PRV) proliferation by affecting PRV entry and lipid -regulated clathrin -coated pits dynamics [7]. Postnatal depletion of TMEM41B in a CKO mouse model arrests weight gain, causes motor dysfunction, and leads to death, and it severely affects cell proliferation in mouse embryonic fibroblasts [8].
In conclusion, TMEM41B is a multifunctional protein essential for processes like lipoprotein biogenesis, lipid homeostasis, and autophagosome formation. Its role in facilitating viral infections, especially those of flaviviruses and coronaviruses, has been well -demonstrated through KO/CKO mouse models. These models have also shown its importance in lipid -related diseases such as non -alcoholic hepatosteatosis and in the context of viral diseases like PRV infection, providing potential therapeutic targets for these conditions.
References:
1. Hoffmann, H-Heinrich, Schneider, William M, Rozen-Gagnon, Kathryn, Poirier, John T, Rice, Charles M. 2020. TMEM41B Is a Pan-flavivirus Host Factor. In Cell, 184, 133-148.e20. doi:10.1016/j.cell.2020.12.005. https://pubmed.ncbi.nlm.nih.gov/33338421/
2. Huang, Dong, Xu, Bolin, Liu, Lu, Reinisch, Karin, Chen, Xiao-Wei. 2021. TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis. In Cell metabolism, 33, 1655-1670.e8. doi:10.1016/j.cmet.2021.05.006. https://pubmed.ncbi.nlm.nih.gov/34015269/
3. Ji, Mingming, Li, Meng, Sun, Long, Deng, Hongyu, Zhao, Yan G. 2022. VMP1 and TMEM41B are essential for DMV formation during β-coronavirus infection. In The Journal of cell biology, 221, . doi:10.1083/jcb.202112081. https://pubmed.ncbi.nlm.nih.gov/35536318/
4. Zhang, Tizhong, Li, Yang E, Yuan, Yiqiong, Yang, Hongyuan, Qi, Shiqian. 2021. TMEM41B and VMP1 are phospholipid scramblases. In Autophagy, 17, 2048-2050. doi:10.1080/15548627.2021.1937898. https://pubmed.ncbi.nlm.nih.gov/34074213/
5. Li, Yang Emma, Wang, Yichang, Du, Ximing, Qi, Shiqian, Yang, Hongyuan. . TMEM41B and VMP1 are scramblases and regulate the distribution of cholesterol and phosphatidylserine. In The Journal of cell biology, 220, . doi:10.1083/jcb.202103105. https://pubmed.ncbi.nlm.nih.gov/33929485/
6. Yousefi, Meisam, Lee, Wai Suet, Yan, Biaoguo, Chan, Kuan Rong, Ooi, Yaw Shin. 2022. TMEM41B and VMP1 modulate cellular lipid and energy metabolism for facilitating dengue virus infection. In PLoS pathogens, 18, e1010763. doi:10.1371/journal.ppat.1010763. https://pubmed.ncbi.nlm.nih.gov/35939522/
7. Li, Xiu-Qing, Zeng, Lei, Liang, Dong-Ge, Chu, Bei-Bei, Wang, Jiang. 2023. TMEM41B Is an Interferon-Stimulated Gene That Promotes Pseudorabies Virus Replication. In Journal of virology, 97, e0041223. doi:10.1128/jvi.00412-23. https://pubmed.ncbi.nlm.nih.gov/37255475/
8. Carlini, Maria J, Van Alstyne, Meaghan, Yang, Hua, Shneider, Neil A, Pellizzoni, Livio. 2024. Stasimon/Tmem41b is required for cell proliferation and adult mouse survival. In Biochemical and biophysical research communications, 712-713, 149923. doi:10.1016/j.bbrc.2024.149923. https://pubmed.ncbi.nlm.nih.gov/38640735/
9. Morita, Keigo, Hama, Yutaro, Mizushima, Noboru. 2019. TMEM41B functions with VMP1 in autophagosome formation. In Autophagy, 15, 922-923. doi:10.1080/15548627.2019.1582952. https://pubmed.ncbi.nlm.nih.gov/30773971/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen