C57BL/6JCya-Sarm1em1flox/Cya
Common Name:
Sarm1-flox
Product ID:
S-CKO-07960
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Sarm1-flox
Strain ID
CKOCMP-237868-Sarm1-B6J-VA
Gene Name
Product ID
S-CKO-07960
Gene Alias
A830091I15Rik; MyD885; Sarm
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sarm1em1flox/Cya mice (Catalog S-CKO-07960) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000108287
NCBI RefSeq
NM_001168521
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Sarm1, short for sterile alpha and TIR motif-containing 1, is a key mediator in both the immune and nervous systems. It functions as a nicotinamide adenine dinucleotide (NAD+)-cleaving enzyme. Its activation is associated with axon degeneration, and it also plays roles in pathways like NF-κB signaling. Genetic models, such as knockout and conditional knockout mice, have been crucial for studying Sarm1's functions [2,3,6].
In spinal cord injury (SCI) mouse models, conditional knockout (CKO) of Sarm1 in the central nervous system (CNS) using Nestin-Cre and GFAP-Cre transgenic mice crossed with Sarm1flox/flox mice promoted neuronal regeneration and functional recovery. This was achieved through reduced neuroinflammation at the early phase of SCI via down-regulation of NF-κB signaling, potentially due to up-regulation of HSP70 [1].
In Alzheimer's disease (AD) model mice, CKO of Sarm1 in the CNS (Sarm1Nestin-CKO mice) delayed cognitive decline, reduced Aβ deposition, and inhibited neurodegeneration by down-regulating TNF-α signaling [4].
In paclitaxel-induced peripheral neuropathy, Sarm1 knockout mice showed prevention of loss of axonal function in a gene-dosage-dependent manner, and pharmacological inhibition of Sarm1 protected axon structure and function [5].
In conclusion, Sarm1 is a significant factor in axon degeneration and neuroinflammation. Studies using Sarm1 KO/CKO mouse models have revealed its roles in diseases like spinal cord injury, Alzheimer's disease, and peripheral neuropathy, suggesting that targeting Sarm1 could be a viable therapeutic approach for these neurological conditions.
References:
1. Liu, Huitao, Zhang, Jingjing, Xu, Xingxing, Huang, Zhihui, Teng, Honglin. 2021. SARM1 promotes neuroinflammation and inhibits neural regeneration after spinal cord injury through NF-κB signaling. In Theranostics, 11, 4187-4206. doi:10.7150/thno.49054. https://pubmed.ncbi.nlm.nih.gov/33754056/
2. Figley, Matthew D, Gu, Weixi, Nanson, Jeffrey D, DiAntonio, Aaron, Ve, Thomas. 2021. SARM1 is a metabolic sensor activated by an increased NMN/NAD+ ratio to trigger axon degeneration. In Neuron, 109, 1118-1136.e11. doi:10.1016/j.neuron.2021.02.009. https://pubmed.ncbi.nlm.nih.gov/33657413/
3. Sambashivan, Shilpa, Freeman, Marc R. 2021. SARM1 signaling mechanisms in the injured nervous system. In Current opinion in neurobiology, 69, 247-255. doi:10.1016/j.conb.2021.05.004. https://pubmed.ncbi.nlm.nih.gov/34175654/
4. Miao, Xuemeng, Wu, Qian, Du, Siyu, Wang, Ying, Huang, Zhihui. 2024. SARM1 Promotes Neurodegeneration and Memory Impairment in Mouse Models of Alzheimer's Disease. In Aging and disease, 15, 390-407. doi:10.14336/AD.2023.0516-1. https://pubmed.ncbi.nlm.nih.gov/37307837/
5. Bosanac, Todd, Hughes, Robert O, Engber, Thomas, Bentley, Jonathan, Krauss, Raul. . Pharmacological SARM1 inhibition protects axon structure and function in paclitaxel-induced peripheral neuropathy. In Brain : a journal of neurology, 144, 3226-3238. doi:10.1093/brain/awab184. https://pubmed.ncbi.nlm.nih.gov/33964142/
6. McGuinness, Helen Y, Gu, Weixi, Shi, Yun, Kobe, Bostjan, Ve, Thomas. 2023. SARM1-Dependent Axon Degeneration: Nucleotide Signaling, Neurodegenerative Disorders, Toxicity, and Therapeutic Opportunities. In The Neuroscientist : a review journal bringing neurobiology, neurology and psychiatry, 30, 473-492. doi:10.1177/10738584231162508. https://pubmed.ncbi.nlm.nih.gov/37002660/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen