C57BL/6JCya-Fscn2em1flox/Cya
Common Name:
Fscn2-flox
Product ID:
S-CKO-07985
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Fscn2-flox
Strain ID
CKOCMP-238021-Fscn2-B6J-VA
Gene Name
Product ID
S-CKO-07985
Gene Alias
A930022G03; Ahl8; C630046B20Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fscn2em1flox/Cya mice (Catalog S-CKO-07985) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000026445
NCBI RefSeq
NM_172802
Target Region
Exon 2
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Fscn2, also known as Fascin2, encodes an actin cross-linking protein. It is involved in the formation of hair cell stereocilia and retina structure [2]. The gene may play a role in regulating cell-related processes such as proliferation and migration through its influence on certain pathways. Genetic models, like gene-knockout mouse models, are valuable for studying Fscn2.
In Fscn2 knockout mice, there is progressive loss of hearing and hair cells, along with upregulation of PARVB, which leads to inhibition of the ILK-AKT pathway and cochlear cell death [2]. These mice also show increased susceptibility to cisplatin-induced hearing impairment and cochlear cell apoptosis [3]. In terms of the retina, haploinsufficiency of Fscn2 hampers the maintenance and/or elongation of the outer segment disks, resulting in photoreceptor degeneration, similar to human autosomal dominant retinitis pigmentosa [1].
In conclusion, Fscn2 is essential for maintaining ear and eye function. Gene-knockout mouse models have revealed its role in preventing cochlear cell death, protecting against cisplatin-induced ototoxicity, and maintaining the structure and function of the retina. These findings contribute to understanding the mechanisms of genetic hearing loss and retinal degeneration.
References:
1. Yokokura, Shunji, Wada, Yuko, Nakai, Shigeyasu, Tamai, Makoto, Noda, Tetsuo. . Targeted disruption of FSCN2 gene induces retinopathy in mice. In Investigative ophthalmology & visual science, 46, 2905-15. doi:. https://pubmed.ncbi.nlm.nih.gov/16043865/
2. Liu, Rongrong, Shang, Wenjing, Liu, Yingying, Song, Xicheng, Han, Fengchan. 2024. Inhibition of the ILK-AKT pathway by upregulation of PARVB contributes to the cochlear cell death in Fascin2 gene knockout mice. In Cell death discovery, 10, 89. doi:10.1038/s41420-024-01851-5. https://pubmed.ncbi.nlm.nih.gov/38374196/
3. Wang, Yan, Liu, Yingying, Xie, Yi, Shang, Wenjing, Han, Fengchan. 2024. Loss of Fascin2 increases susceptibility to cisplatin-induced hearing impairment and cochlear cell apoptosis in mice. In Journal of otology, 19, 133-139. doi:10.1016/j.joto.2024.07.001. https://pubmed.ncbi.nlm.nih.gov/39735238/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen