C57BL/6JCya-Cacna1iem1flox/Cya
Common Name:
Cacna1i-flox
Product ID:
S-CKO-08144
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cacna1i-flox
Strain ID
CKOCMP-239556-Cacna1i-B6J-VA
Gene Name
Product ID
S-CKO-08144
Gene Alias
Ca(V)3.3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cacna1iem1flox/Cya mice (Catalog S-CKO-08144) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000160424
NCBI RefSeq
NM_001044308
Target Region
Exon 3
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
CACNA1I encodes the Cav3.3 channel, a member of the T-type calcium channel family (Cav3.1-Cav3.3). These channels regulate low-threshold calcium spikes, burst firing, and rhythmic oscillations of neurons, and are involved in sensory processing, sleep, and hormone and neurotransmitter release [1,3].
Gain-of-function mutations in CACNA1I can cause neurodevelopmental disorders. Mutations such as p.(Ile860Met), p.(Ile860Asn), p.(Ile1306Thr), and p.(Met1425Ile) lead to slowed kinetics of current activation, inactivation, and deactivation, and hyperpolarizing shifts of voltage-dependence of activation and inactivation. This results in increased calcium influx, potentially causing calcium toxicity in neurons and neuronal hyper-excitability, which can explain symptoms like seizures [1].
In a Swedish schizophrenia cohort, rare CACNA1I alleles from schizophrenia patients encoded CaV3.3 channels with altered responses to voltages, while rare alleles from control subjects were associated with reduced CaV3.3 channel current density. Reduced CaV3.3 function may protect against schizophrenia risk in rare cases [4]. Also, there is evidence suggesting an increased burden of missense variants in CACNA1I in hemiplegic migraine patients, and some studies have associated it with schizophrenia in Chinese populations [2,5,6,7].
In conclusion, CACNA1I is crucial for normal neuronal function through its role in T-type calcium channel activity. Studies on its gain-and loss-of-function mutations, especially in relation to neurodevelopmental disorders and schizophrenia, have provided insights into the molecular mechanisms underlying these diseases. Understanding CACNA1I can potentially lead to new therapeutic strategies for these neurological conditions.
References:
1. El Ghaleb, Yousra, Schneeberger, Pauline E, Fernández-Quintero, Monica L, Kutsche, Kerstin, Flucher, Bernhard E. . CACNA1I gain-of-function mutations differentially affect channel gating and cause neurodevelopmental disorders. In Brain : a journal of neurology, 144, 2092-2106. doi:10.1093/brain/awab101. https://pubmed.ncbi.nlm.nih.gov/33704440/
2. Maksemous, Neven, Blayney, Claire D, Sutherland, Heidi G, Adams, David J, Griffiths, Lyn R. 2022. Investigation of CACNA1I Cav3.3 Dysfunction in Hemiplegic Migraine. In Frontiers in molecular neuroscience, 15, 892820. doi:10.3389/fnmol.2022.892820. https://pubmed.ncbi.nlm.nih.gov/35928792/
3. El Ghaleb, Yousra, Flucher, Bernhard E. . CaV3.3 Channelopathies. In Handbook of experimental pharmacology, 279, 263-288. doi:10.1007/164_2022_631. https://pubmed.ncbi.nlm.nih.gov/36592228/
4. Baez-Nieto, David, Allen, Andrew, Akers-Campbell, Seth, Lipscombe, Diane, Pan, Jen Q. . Analysing an allelic series of rare missense variants of CACNA1I in a Swedish schizophrenia cohort. In Brain : a journal of neurology, 145, 1839-1853. doi:10.1093/brain/awab443. https://pubmed.ncbi.nlm.nih.gov/34919654/
5. Xie, Yijun, Huang, Di, Wei, Li, Luo, Xiong-Jian. 2018. Further evidence for the genetic association between CACNA1I and schizophrenia. In Hereditas, 155, 16. doi:10.1186/s41065-017-0054-0. https://pubmed.ncbi.nlm.nih.gov/29308060/
6. Maksemous, Neven, Harder, Aster V E, Ibrahim, Omar, van den Maagdenberg, Arn M J M, Griffiths, Lyn R. 2023. Whole Exome Sequencing of Hemiplegic Migraine Patients Shows an Increased Burden of Missense Variants in CACNA1H and CACNA1I Genes. In Molecular neurobiology, 60, 3034-3043. doi:10.1007/s12035-023-03255-5. https://pubmed.ncbi.nlm.nih.gov/36786913/
7. Xu, Wei, Liu, Yahui, Chen, Jianhua, Yi, Qizhong, Shi, Yongyong. 2017. Genetic risk between the CACNA1I gene and schizophrenia in Chinese Uygur population. In Hereditas, 155, 5. doi:10.1186/s41065-017-0037-1. https://pubmed.ncbi.nlm.nih.gov/28725167/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen