C57BL/6JCya-Igf2bp2em1flox/Cya
Common Name:
Igf2bp2-flox
Product ID:
S-CKO-10339
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Igf2bp2-flox
Strain ID
CKOCMP-319765-Igf2bp2-B6J-VA
Gene Name
Product ID
S-CKO-10339
Gene Alias
C330012H03Rik; IMP-2; Imp2; Neilsen
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Igf2bp2em1flox/Cya mice (Catalog S-CKO-10339) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000100052
NCBI RefSeq
NM_183029
Target Region
Exon 3~4
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Igf2bp2, also known as insulin-like growth factor 2 mRNA binding protein 2 (IGF2BP2/IMP2), is an RNA-binding protein and an N6-methyladenosine (m6A) reader. It plays a crucial role in regulating multiple biological processes. It is involved in post-transcriptional regulation of numerous genes, influencing pathways such as glutamine metabolism, cell cycle progression, angiogenesis, and metastasis. It is of great biological importance as it participates in human metabolic diseases like diabetes, obesity, and fatty liver, as well as in the development and progression of various cancers [2].
In acute myeloid leukemia (AML), high expression of Igf2bp2 is associated with unfavorable prognosis. Knockdown of Igf2bp2 using a small-molecule compound (CWI1-2) shows anti-leukemia effects both in vitro and in vivo. Igf2bp2 promotes AML development and self-renewal of leukemia stem/initiation cells by regulating critical targets in the glutamine metabolism pathways in an m6A-dependent manner [1].
In lung adenocarcinoma, Igf2bp2 is specifically expressed in certain cell subpopulations. Its knockdown may potentially inhibit angiogenesis and metastasis, as it activates endothelial cells to promote these processes through an m6A-mediated mechanism [3].
In triple-negative breast cancer (TNBC), Igf2bp2 is highly expressed. Downregulation of Igf2bp2 in TNBC cells enhances their sensitivity to abemaciclib, a CDK4/6 inhibitor. Igf2bp2 promotes TNBC proliferation and cell cycle transition via directly regulating CDK6 in an m6A-dependent manner [4].
In conclusion, Igf2bp2, as an m6A reader, is involved in multiple biological processes and diseases. Through gene-knockout or knockdown models in various disease settings such as AML, lung adenocarcinoma, and TNBC, its role in promoting disease progression has been revealed. These findings suggest that targeting Igf2bp2 could be a promising therapeutic strategy for these diseases.
References:
1. Weng, Hengyou, Huang, Feng, Yu, Zhaojin, Huang, Huilin, Chen, Jianjun. 2022. The m6A reader IGF2BP2 regulates glutamine metabolism and represents a therapeutic target in acute myeloid leukemia. In Cancer cell, 40, 1566-1582.e10. doi:10.1016/j.ccell.2022.10.004. https://pubmed.ncbi.nlm.nih.gov/36306790/
2. Wang, Jinyan, Chen, Lijuan, Qiang, Ping. 2021. The role of IGF2BP2, an m6A reader gene, in human metabolic diseases and cancers. In Cancer cell international, 21, 99. doi:10.1186/s12935-021-01799-x. https://pubmed.ncbi.nlm.nih.gov/33568150/
3. Fang, Han, Sun, Qi, Zhou, Jin, Yu, Xiaofeng, Song, Xicheng. 2023. m6A methylation reader IGF2BP2 activates endothelial cells to promote angiogenesis and metastasis of lung adenocarcinoma. In Molecular cancer, 22, 99. doi:10.1186/s12943-023-01791-1. https://pubmed.ncbi.nlm.nih.gov/37353784/
4. Xia, Tian, Dai, Xin-Yuan, Sang, Ming-Yi, Wei, Ji-Fu, Ding, Qiang. 2023. IGF2BP2 Drives Cell Cycle Progression in Triple-Negative Breast Cancer by Recruiting EIF4A1 to Promote the m6A-Modified CDK6 Translation Initiation Process. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2305142. doi:10.1002/advs.202305142. https://pubmed.ncbi.nlm.nih.gov/37983610/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen