C57BL/6JCya-Gpr183em1flox/Cya
Common Name:
Gpr183-flox
Product ID:
S-CKO-10527
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Gpr183-flox
Strain ID
CKOCMP-321019-Gpr183-B6J-VA
Gene Name
Product ID
S-CKO-10527
Gene Alias
Ebi2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gpr183em1flox/Cya mice (Catalog S-CKO-10527) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000049872
NCBI RefSeq
NM_183031
Target Region
Exon 2
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Gpr183, also known as Epstein-Barr virus-induced gene 2, is a G protein-coupled receptor for oxysterols and hydroxylated metabolites of cholesterol. It plays pleiotropic roles in lipid metabolism, immune responses, and is involved in multiple biological processes such as immune cell positioning, organ development, and tissue homeostasis [3,6]. Oxysterols, like 7α,25-dihydroxycholesterol (7α,25-OHC), bind to Gpr183, and the receptor-ligand pair is associated with various diseases [6].
Loss-of-function mutation of Gpr183 in mice has shown significant impacts. In influenza A virus and SARS-CoV-2 infections, it reduced macrophage infiltration and inflammatory cytokine production in the lungs, attenuating the severity of SARS-CoV-2 infection and viral loads [1]. In group 3 innate lymphoid cells (ILC3s), Gpr183 deficiency caused a defect in the formation of cryptopatches and isolated lymphoid follicles in the colon, affecting colonic lymphoid tissue development and colitis [2]. In endothelial-specific Gpr183 knockout mice, it alleviated cardiovascular and renal injuries in hypertensive mice by regulating endothelial senescence [3]. In IBD-related colitis models, Gpr183 inactivation reduced the severity of colitis in some models and strongly reduced the accumulation of intestinal lymphoid tissue [4]. In Mycobacterium tuberculosis infection, Gpr183-deficient mice had increased lung Mtb burden and dysregulated interferons during early infection [5].
In conclusion, Gpr183 is crucial in immune-related and disease-associated processes. Gene knockout mouse models have been instrumental in revealing its role in viral respiratory infections, colonic inflammation, hypertension-related complications, inflammatory bowel diseases, and mycobacterial infections. These findings suggest that targeting Gpr183 could be a potential therapeutic strategy for these diseases [1,2,3,4,5].
References:
1. Foo, Cheng Xiang, Bartlett, Stacey, Chew, Keng Yih, Short, Kirsty R, Ronacher, Katharina. 2023. GPR183 antagonism reduces macrophage infiltration in influenza and SARS-CoV-2 infection. In The European respiratory journal, 61, . doi:10.1183/13993003.01306-2022. https://pubmed.ncbi.nlm.nih.gov/36396144/
2. Emgård, Johanna, Kammoun, Hana, García-Cassani, Bethania, Flavell, Richard A, Willinger, Tim. . Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation. In Immunity, 48, 120-132.e8. doi:10.1016/j.immuni.2017.11.020. https://pubmed.ncbi.nlm.nih.gov/29343433/
3. Chu, Qingqing, Li, Yujia, Wu, Jichao, Wang, Xiaojie, Yi, Fan. 2024. Oxysterol Sensing Through GPR183 Triggers Endothelial Senescence in Hypertension. In Circulation research, 135, 708-721. doi:10.1161/CIRCRESAHA.124.324722. https://pubmed.ncbi.nlm.nih.gov/39176657/
4. Misselwitz, Benjamin, Wyss, Annika, Raselli, Tina, Pot, Caroline, Pabst, Oliver. 2021. The oxysterol receptor GPR183 in inflammatory bowel diseases. In British journal of pharmacology, 178, 3140-3156. doi:10.1111/bph.15311. https://pubmed.ncbi.nlm.nih.gov/33145756/
5. Bartlett, Stacey, Gemiarto, Adrian Tandhyka, Ngo, Minh Dao, Mandrup-Poulsen, Thomas, Ronacher, Katharina. 2020. GPR183 Regulates Interferons, Autophagy, and Bacterial Growth During Mycobacterium tuberculosis Infection and Is Associated With TB Disease Severity. In Frontiers in immunology, 11, 601534. doi:10.3389/fimmu.2020.601534. https://pubmed.ncbi.nlm.nih.gov/33240287/
6. Kjær, Viktoria M S, Daugvilaite, Viktorija, Stepniewski, Tomasz M, Selent, Jana, Rosenkilde, Mette M. 2023. Migration mediated by the oxysterol receptor GPR183 depends on arrestin coupling but not receptor internalization. In Science signaling, 16, eabl4283. doi:10.1126/scisignal.abl4283. https://pubmed.ncbi.nlm.nih.gov/37014928/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen