C57BL/6JCya-Uprtem1flox/Cya
Common Name:
Uprt-flox
Product ID:
S-CKO-10729
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Uprt-flox
Strain ID
CKOCMP-331487-Uprt-B6J-VA
Gene Name
Product ID
S-CKO-10729
Gene Alias
D830012I24Rik; Gm774
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Uprtem1flox/Cya mice (Catalog S-CKO-10729) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000087867
NCBI RefSeq
NM_001081189
Target Region
Exon 3
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Uprt, short for uracil phosphoribosyltransferase, is an enzyme in the pyrimidine salvage pathway. It catalyzes the conversion of uracil to uridine monophosphate (UMP), and is highly conserved across species from prokaryotes to humans [1].
In Drosophila, the Uprt homologue, krishah (kri), is essential for larval growth, pre-pupal/pupal viability, and long-term adult lifespan, suggesting that Uprt in higher eukaryotes likely has in-vivo enzymatic activity [1].
In gene therapy, Uprt is part of the CD/UPRT system. For example, vaccinia virus armed with CD/UPRT can convert 5-fluorocytosine (5-FC) into chemotherapeutic agents, showing anti-tumor effects against pancreatic ductal adenocarcinoma [2]. In glioma gene therapy, UPRT in the CD::UPRT/5-FC system can catalyze 5-fluorouracil (5-FU) to 5-fluorouridine monophosphate, which kills cells via the bystander effect [3]. Engineered oncolytic adenovirus expressing UPRT can enhance the toxicity of 5-FU, and cyclophosphamide can further improve its antitumor efficacy in immunocompetent Syrian hamsters [4]. Also, expressing AtUPRT from Arabidopsis thaliana in HeLa cells makes them more sensitive to 5-FU, leading to decreased cell viability, apoptosis, and cell cycle arrest [5].
In conclusion, Uprt plays a crucial role in pyrimidine metabolism and has significant implications in cancer gene therapy. Studies in model organisms like Drosophila and gene-therapy-related cell models have revealed its importance in growth, survival, and tumor-targeted treatment, highlighting its potential as a therapeutic target or tool in cancer treatment.
References:
1. Ghosh, Arpan C, Shimell, MaryJane, Leof, Emma R, Haley, Macy J, O'Connor, Michael B. 2015. UPRT, a suicide-gene therapy candidate in higher eukaryotes, is required for Drosophila larval growth and normal adult lifespan. In Scientific reports, 5, 13176. doi:10.1038/srep13176. https://pubmed.ncbi.nlm.nih.gov/26271729/
2. Kurosaki, Hajime, Nakatake, Motomu, Sakamoto, Teruhisa, Fujiwara, Yoshiyuki, Nakamura, Takafumi. 2021. Anti-Tumor Effects of MAPK-Dependent Tumor-Selective Oncolytic Vaccinia Virus Armed with CD/UPRT against Pancreatic Ductal Adenocarcinoma in Mice. In Cells, 10, . doi:10.3390/cells10050985. https://pubmed.ncbi.nlm.nih.gov/33922406/
3. Shi, De-zhi, Hu, Wei-xing, Li, Li-xin, Wei, Dong, Gu, Pei-yuan. . Pharmacokinetics and the bystander effect in CD::UPRT/5-FC bi-gene therapy of glioma. In Chinese medical journal, 122, 1267-72. doi:. https://pubmed.ncbi.nlm.nih.gov/19567135/
4. Hasegawa, Naoyuki, Abei, Masato, Yokoyama, Kazunari K, Obata, Yuichi, Hyodo, Ichinosuke. 2013. Cyclophosphamide enhances antitumor efficacy of oncolytic adenovirus expressing uracil phosphoribosyltransferase (UPRT) in immunocompetent Syrian hamsters. In International journal of cancer, 133, 1479-88. doi:10.1002/ijc.28132. https://pubmed.ncbi.nlm.nih.gov/23444104/
5. Narayanan, Sharmila, Sanpui, Pallab, Sahoo, Lingaraj, Ghosh, Siddhartha Sankar. 2016. Unravelling the potential of a new uracil phosphoribosyltransferase (UPRT) from Arabidopsis thaliana in sensitizing HeLa cells towards 5-fluorouracil. In International journal of biological macromolecules, 91, 310-6. doi:10.1016/j.ijbiomac.2016.05.037. https://pubmed.ncbi.nlm.nih.gov/27180296/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen