C57BL/6JCya-Peds1em1flox/Cya
Common Name:
Peds1-flox
Product ID:
S-CKO-11188
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Peds1-flox
Strain ID
CKOCMP-407243-Peds1-B6J-VA
Gene Name
Product ID
S-CKO-11188
Gene Alias
Kua; Tmem189
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Peds1em1flox/Cya mice (Catalog S-CKO-11188) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000006587
NCBI RefSeq
NM_145538
Target Region
Exon 3
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Peds1, also known as plasmanylethanolamine desaturase 1 (PEDS1) and TMEM189, is an enzyme crucial for plasmalogen biosynthesis. Plasmalogens are glycerophospholipids with a vinyl ether bond, and Peds1 functions to introduce this double bond into plasmanylethanolamine phospholipids [2,3,4,7]. Ether lipids, including plasmalogens, play roles in membrane organization, fluidity, signaling, and antioxidative functions, and abnormal levels are associated with various human pathologies [2,8].
Peds1 -deficient zebrafish larvae display delayed development, increased basal inflammation, normal hematopoietic stem and progenitor cell emergence, and cell-autonomous myeloid cell apoptosis. In inflammation models, they show impaired inflammation resolution, tissue regeneration, increased cytokine expression, and elevated ROS levels. Exogenous plasmalogen can mitigate some of these effects [1]. Peds1-knockout mice exhibit a growth phenotype and striking changes in lipid composition, with a strong reduction of plasmenyl lipids and a concomitant massive accumulation of plasmanyl lipids [3]. A single mutation (aspartate 100) in murine Peds1 leads to a total loss of its enzymatic activity [4]. In humans, the Peds1 rs6020298 GG genotype and G allele are significantly associated with an increased risk of developing Long COVID [5]. TMEM189 (Peds1) negatively regulates the proteostasis of ULK1 and autophagy activity, and its deficiency inhibits the tumorigenicity of gastric cancer [6].
In conclusion, Peds1 is essential for plasmalogen biosynthesis, and its deficiency in model organisms reveals its importance in myeloid cell biology, inflammation, lipid metabolism, autophagy, and disease-related processes such as Long COVID and tumorigenesis. The study of Peds1 through gene-knockout models provides valuable insights into its biological functions and potential implications for human health.
References:
1. Arroyo, Ana B, Tyrkalska, Sylwia D, Bastida-Martínez, Eva, Elías-Arnanz, Montserrat, Mulero, Victoriano. 2024. Peds1 deficiency in zebrafish results in myeloid cell apoptosis and exacerbated inflammation. In Cell death discovery, 10, 388. doi:10.1038/s41420-024-02141-w. https://pubmed.ncbi.nlm.nih.gov/39209813/
2. Padmanabhan, S, Monera-Girona, Antonio J, Pajares-Martínez, Elena, Fontes, Marta, Elías-Arnanz, Montserrat. 2022. Plasmalogens and Photooxidative Stress Signaling in Myxobacteria, and How it Unmasked CarF/TMEM189 as the Δ1'-Desaturase PEDS1 for Human Plasmalogen Biosynthesis. In Frontiers in cell and developmental biology, 10, 884689. doi:10.3389/fcell.2022.884689. https://pubmed.ncbi.nlm.nih.gov/35646900/
3. Lackner, Katharina, Sailer, Sabrina, van Klinken, Jan-Bert, Werner, Ernst R, Watschinger, Katrin. 2023. Alterations in ether lipid metabolism and the consequences for the mouse lipidome. In Biochimica et biophysica acta. Molecular and cell biology of lipids, 1868, 159285. doi:10.1016/j.bbalip.2023.159285. https://pubmed.ncbi.nlm.nih.gov/36690320/
4. Werner, Ernst R, Fernández-Quintero, Monica L, Hulo, Nicolas, Liedl, Klaus R, Watschinger, Katrin. 2022. Essential role of a conserved aspartate for the enzymatic activity of plasmanylethanolamine desaturase. In Cellular and molecular life sciences : CMLS, 79, 214. doi:10.1007/s00018-022-04238-w. https://pubmed.ncbi.nlm.nih.gov/35347434/
5. Udomsinprasert, Wanvisa, Nontawong, Nuttakant, Saengsiwaritt, Wacharapol, Chaikledkaew, Usa, Chantratita, Wasun. . Host genetic polymorphisms involved in long-term symptoms of COVID-19. In Emerging microbes & infections, 12, 2239952. doi:10.1080/22221751.2023.2239952. https://pubmed.ncbi.nlm.nih.gov/37497655/
6. Yu, Jiahong, Qu, Liujing, Xia, Yan, Lu, Wenping, Chen, Yingyu. 2022. TMEM189 negatively regulates the stability of ULK1 protein and cell autophagy. In Cell death & disease, 13, 316. doi:10.1038/s41419-022-04722-y. https://pubmed.ncbi.nlm.nih.gov/35393404/
7. Costa, Joaquín, Gabrielli, Matías, Altabe, Silvia G, Uttaro, Antonio D. 2024. The presence of plasmenyl ether lipids in Capsaspora owczarzaki suggests a premetazoan origin of plasmalogen biosynthesis in animals. In Heliyon, 10, e32807. doi:10.1016/j.heliyon.2024.e32807. https://pubmed.ncbi.nlm.nih.gov/38975177/
8. Koch, Jakob, Watschinger, Katrin, Werner, Ernst R, Keller, Markus A. 2022. Tricky Isomers-The Evolution of Analytical Strategies to Characterize Plasmalogens and Plasmanyl Ether Lipids. In Frontiers in cell and developmental biology, 10, 864716. doi:10.3389/fcell.2022.864716. https://pubmed.ncbi.nlm.nih.gov/35573699/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen