C57BL/6JCya-Cks1bem1flox/Cya
Common Name:
Cks1b-flox
Product ID:
S-CKO-11679
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cks1b-flox
Strain ID
CKOCMP-54124-Cks1b-B6J-VA
Gene Name
Product ID
S-CKO-11679
Gene Alias
2410005G18Rik; 2610005D03Rik; Cks1; sid1334
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cks1bem1flox/Cya mice (Catalog S-CKO-11679) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029679
NCBI RefSeq
NM_016904
Target Region
Exon 2~3
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Cks1b, Cyclin-dependent kinase regulatory subunit 1B, is a member of the conserved cyclin kinase subunit 1 (CKS1) protein family. It plays an essential role in cell cycling and is involved in multiple signaling pathways, such as JAK/STAT3, MEK/ERK, and the cell cycle-related ATR-Chk1-Cdc25 pathway. Its biological importance extends to cancer pathogenesis and the regulation of cardiomyocyte proliferation-maturation transition [1,2].
In cancer research, loss-of-function experiments have been revealing. For example, knockdown of Cks1b in hepatocellular carcinoma cell lines inhibited cell survival, proliferation, migration, invasion, and induced apoptosis, and it was shown to do so by inhibiting the JAK/STAT3 pathway [3]. In retinoblastoma cells, shRNA-mediated downregulation of Cks1b restrained cell proliferation, migration, invasion, and angiogenesis through suppressing the MEK/ERK signaling pathway [6]. In pancreatic cancer, Cks1b knockdown reduced cell viability and invasion, and also sensitized cells to gemcitabine and oxaliplatin treatment [4,5].
In conclusion, Cks1b is crucial for normal cell cycling and its dysregulation is implicated in various cancers. The gene knockout or knockdown models have demonstrated its oncogenic role in promoting cancer cell proliferation, migration, and invasion, as well as its association with drug resistance. These findings suggest that Cks1b could be a potential therapeutic target for cancer treatment [1,3-8].
References:
1. Shi, Wenwen, Huang, Qiudi, Xie, Jiacui, Yu, Xiyong, Zhou, Yi. 2020. CKS1B as Drug Resistance-Inducing Gene-A Potential Target to Improve Cancer Therapy. In Frontiers in oncology, 10, 582451. doi:10.3389/fonc.2020.582451. https://pubmed.ncbi.nlm.nih.gov/33102238/
2. Waldron, Christina J, Kelly, Lauren A, Stan, Nicholas, Ogle, Brenda M, Singh, Bhairab N. . The HH-GLI2-CKS1B network regulates the proliferation-to-maturation transition of cardiomyocytes. In Stem cells translational medicine, 13, 678-692. doi:10.1093/stcltm/szae032. https://pubmed.ncbi.nlm.nih.gov/38761090/
3. Liu, Xitao, Zhao, Defang. 2021. CKS1B promotes the progression of hepatocellular carcinoma by activating JAK/STAT3 signal pathway. In Animal cells and systems, 25, 227-234. doi:10.1080/19768354.2021.1953142. https://pubmed.ncbi.nlm.nih.gov/34408811/
4. Li, Lincheng, Wang, Jing, Zhang, Zhuochao, Li, Chonghui, Liu, Rong. 2022. Identification of CKS1B as a prognostic indicator and a predictive marker for immunotherapy in pancreatic cancer. In Frontiers in immunology, 13, 1052768. doi:10.3389/fimmu.2022.1052768. https://pubmed.ncbi.nlm.nih.gov/36405738/
5. Zhang, Liuxi, Wei, Fang, Sun, Qihui, Xie, Keping, He, Jie. 2025. FOXM1-Driven CKS1B Upregulation Promotes Pancreatic Cancer Progression and Therapeutic Resistance. In International journal of biological sciences, 21, 1047-1064. doi:10.7150/ijbs.105289. https://pubmed.ncbi.nlm.nih.gov/39897042/
6. Zeng, Zhou, Gao, Zhao-Lin, Zhang, Zhi-Pei, Yang, Jie, Xia, Xiao-Bo. 2019. Downregulation of CKS1B restrains the proliferation, migration, invasion and angiogenesis of retinoblastoma cells through the MEK/ERK signaling pathway. In International journal of molecular medicine, 44, 103-114. doi:10.3892/ijmm.2019.4183. https://pubmed.ncbi.nlm.nih.gov/31115482/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen