C57BL/6JCya-Bace2em1flox/Cya
Common Name:
Bace2-flox
Product ID:
S-CKO-11957
Background:
C57BL/6JCya
Product Type
Age
Genotype
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Basic Information
Strain Name
Bace2-flox
Strain ID
CKOCMP-56175-Bace2-B6J-VA
Gene Name
Product ID
S-CKO-11957
Gene Alias
1110059C24Rik; AEPLC; ALP56; ARP1; ASP1; ASP21; BAE2; CDA13; CEAP1; DRAP
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Bace2em1flox/Cya mice (Catalog S-CKO-11957) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000047275
NCBI RefSeq
NM_019517
Target Region
Exon 2
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Bace2, short for β -site APP cleavage enzyme 2, is a protease that plays a crucial role in the proteolytic processing of the amyloid precursor protein (APP). Unlike BACE1, BACE2 cleaves APP within the Aβ domain, preventing the generation of Aβ42 peptides and thus having a non-amyloidogenic role. This places it as a potential therapeutic target in Alzheimer's disease (AD) [1]. It is also involved in the regulation of endothelial nitric oxide synthase (eNOS) function in cerebrovascular endothelium, suggesting a vascular protective role [2,3].
Genetic downregulation of Bace2 with small interfering RNA in human brain microvascular endothelial cells decreased eNOS expression and nitric oxide production, while Bace2-deficient mice showed impaired endothelium-dependent relaxations to acetylcholine and basal cyclic GMP production, indicating its importance in maintaining normal cerebrovascular function [3]. In human pluripotent stem cell-derived brain organoids, a Bace2 loss-of-function mutation led to increased apoptosis and Aβ oligomers, resembling AD-associated phenotypes [4].
In conclusion, Bace2 has a non-amyloidogenic role in APP processing and is crucial for maintaining normal cerebrovascular function. Studies using gene-knockout models, such as the genetic downregulation in cells and Bace2-deficient mice, have revealed its significance in AD-related pathological processes and cerebrovascular health, providing potential directions for therapeutic development in these disease areas.
References:
1. Yeap, Yee Jie, Kandiah, Nagaendran, Nizetic, Dean, Lim, Kah-Leong. . BACE2: A Promising Neuroprotective Candidate for Alzheimer's Disease. In Journal of Alzheimer's disease : JAD, 94, S159-S171. doi:10.3233/JAD-220867. https://pubmed.ncbi.nlm.nih.gov/36463454/
2. Sáez-Valero, Javier, Pérez-González, Rocío. 2023. BACE2 beyond β-processing of APP, its neuroprotective role in cerebrovascular endothelium. In Journal of neurochemistry, 166, 887-890. doi:10.1111/jnc.15940. https://pubmed.ncbi.nlm.nih.gov/37587672/
3. He, Tongrong, d'Uscio, Livius V, Katusic, Zvonimir S. 2023. BACE2 deficiency impairs expression and function of endothelial nitric oxide synthase in brain endothelial cells. In Journal of neurochemistry, 166, 928-942. doi:10.1111/jnc.15929. https://pubmed.ncbi.nlm.nih.gov/37547981/
4. Luo, Juan, Zou, Hailin, Guo, Yibo, Ngan, Elly Sau-Wai, Li, Peng. 2022. BACE2 variant identified from HSCR patient causes AD-like phenotypes in hPSC-derived brain organoids. In Cell death discovery, 8, 47. doi:10.1038/s41420-022-00845-5. https://pubmed.ncbi.nlm.nih.gov/35110536/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen