C57BL/6JCya-Mgst1em1flox/Cya
Common Name:
Mgst1-flox
Product ID:
S-CKO-12171
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mgst1-flox
Strain ID
CKOCMP-56615-Mgst1-B6J-VA
Gene Name
Product ID
S-CKO-12171
Gene Alias
1500002K10Rik; Gst
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mgst1em1flox/Cya mice (Catalog S-CKO-12171) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000008684
NCBI RefSeq
NM_019946
Target Region
Exon 2
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Mgst1, or microsomal glutathione S-transferase 1, is a membrane-bound glutathione transferase with glutathione-dependent transferase and peroxidase activities. It can detoxify reactive intermediates such as metabolic electrophile intermediates and lipophilic hydroperoxides [4]. It is activated by oxidative stress and is expressed in high amounts in the liver, located in the endoplasmic reticulum and mitochondrial membranes. Mgst1 has been shown to be crucial for embryonic development and hematopoiesis in vertebrates [5].
In pancreatic ductal adenocarcinoma (PDAC), Mgst1 was found to be the key molecule against MRTX1133-induced ferroptosis, facilitating resistance to the KRASG12D inhibitor MRTX1133. Increased Mgst1 expression weakened the cytotoxicity of MRTX1133 by inhibiting lipid peroxidation-induced ferroptosis. Knockdown of Mgst1 conferred sensitivity to the inhibitor [1]. In pancreatic cancer cells, genetic depletion of Mgst1 promoted ferroptosis, indicating its role as a redox-sensitive repressor of ferroptosis [2]. In glioma cells, Mgst1 expression was increased and its silencing downregulated the IC50 value of temozolomide (TMZ) and facilitated ferroptosis in TMZ-resistant cells, suggesting its role in conferring TMZ resistance [3].
In conclusion, Mgst1 is essential for embryonic development and hematopoiesis. In diseases like PDAC, pancreatic cancer, and glioma, Mgst1 plays a role in inhibiting ferroptosis, which is related to drug resistance. The study of Mgst1 knockout or knockdown models has provided insights into its function in these disease-related biological processes, helping to understand the mechanisms of disease occurrence and potentially providing new strategies for treatment.
References:
1. Xu, Chungui, Lin, Weihao, Zhang, Qi, Wang, Xiaobing, Du, Chunxia. 2024. MGST1 facilitates novel KRASG12D inhibitor resistance in KRASG12D-mutated pancreatic ductal adenocarcinoma by inhibiting ferroptosis. In Molecular medicine (Cambridge, Mass.), 30, 199. doi:10.1186/s10020-024-00972-y. https://pubmed.ncbi.nlm.nih.gov/39501138/
2. Kuang, Feimei, Liu, Jiao, Xie, Yangchun, Tang, Daolin, Kang, Rui. 2021. MGST1 is a redox-sensitive repressor of ferroptosis in pancreatic cancer cells. In Cell chemical biology, 28, 765-775.e5. doi:10.1016/j.chembiol.2021.01.006. https://pubmed.ncbi.nlm.nih.gov/33539732/
3. Yan, Tengfeng, Hu, Ping, Lv, Shigang, Fang, Hua, Xiao, Bing. 2024. ZNF384 transcriptionally activated MGST1 to confer TMZ resistance of glioma cells by negatively regulating ferroptosis. In Cancer chemotherapy and pharmacology, 94, 323-336. doi:10.1007/s00280-024-04681-5. https://pubmed.ncbi.nlm.nih.gov/38824270/
4. Schaffert, Courtney S. . Role of MGST1 in reactive intermediate-induced injury. In World journal of gastroenterology, 17, 2552-7. doi:10.3748/wjg.v17.i20.2552. https://pubmed.ncbi.nlm.nih.gov/21633660/
5. Bräutigam, Lars, Zhang, Jie, Dreij, Kristian, Morgenstern, Ralf, Johansson, Katarina. 2018. MGST1, a GSH transferase/peroxidase essential for development and hematopoietic stem cell differentiation. In Redox biology, 17, 171-179. doi:10.1016/j.redox.2018.04.013. https://pubmed.ncbi.nlm.nih.gov/29702404/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen