C57BL/6JCya-Magt1em1flox/Cya
Common Name:
Magt1-flox
Product ID:
S-CKO-13489
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Magt1-flox
Strain ID
CKOCMP-67075-Magt1-B6J-VA
Gene Name
Product ID
S-CKO-13489
Gene Alias
2410001C15Rik; 2610529C04Rik; 2810482I07Rik; IAG2; IAP
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
X
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Magt1em1flox/Cya mice (Catalog S-CKO-13489) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000238718
NCBI RefSeq
--
Target Region
Exon 2
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
MagT1, or magnesium transporter 1, is a subunit of the oligosaccharide protein complex with thiol-disulfide oxidoreductase activity, crucial for the N-glycosylation process [1]. It is also significantly involved in magnesium homeostasis, with magnesium being essential for over 600 enzymatic reactions in the human body [3].
In MagT1-deficient mice (Magt1-/y), accelerated occlusive arterial thrombus formation, a shortened bleeding time, and profound brain damage upon focal cerebral ischemia were observed. These defects were due to increased calcium influx, enhanced second wave mediator release, and hyperphosphorylation of key proteins in the platelet activation pathway. Supplementation of MgCl2 or blockade of TRPC6 channel normalized platelet aggregation responses. Haploinsufficiency of TRPC6 in Magt1-/y mice could also normalize GPVI signaling, platelet aggregation, and thrombus formation in vivo, suggesting a functional link between MagT1 and TRPC6 [1]. In XMEN disease patients with MagT1 deficiency, severe platelet dysfunction and impaired platelet glycoprotein N-glycosylation were found, with abnormal platelet shapes, impaired aggregation, and integrin activation, which were corrected after hematopoietic stem cell transplantation [2].
In conclusion, MagT1 is essential for N-glycosylation and magnesium homeostasis. Studies using MagT1-deficient mouse models have revealed its crucial role in platelet function and arterial thrombosis-related processes. Understanding MagT1's functions provides insights into the pathogenesis of diseases such as XMEN disease and potential risks of arterial thrombosis and stroke [1,2].
References:
1. Gotru, Sanjeev Kiran, Mammadova-Bach, Elmina, Sogkas, Georgios, Gudermann, Thomas, Braun, Attila. 2023. MAGT1 Deficiency Dysregulates Platelet Cation Homeostasis and Accelerates Arterial Thrombosis and Ischemic Stroke in Mice. In Arteriosclerosis, thrombosis, and vascular biology, 43, 1494-1509. doi:10.1161/ATVBAHA.122.318115. https://pubmed.ncbi.nlm.nih.gov/37381987/
2. Kauskot, Alexandre, Mallebranche, Coralie, Bruneel, Arnaud, Miot, Charline, Adam, Frédéric. 2023. MAGT1 deficiency in XMEN disease is associated with severe platelet dysfunction and impaired platelet glycoprotein N-glycosylation. In Journal of thrombosis and haemostasis : JTH, 21, 3268-3278. doi:10.1016/j.jtha.2023.05.007. https://pubmed.ncbi.nlm.nih.gov/37207862/
3. de Baaij, Jeroen H F, Hoenderop, Joost G J, Bindels, René J M. . Magnesium in man: implications for health and disease. In Physiological reviews, 95, 1-46. doi:10.1152/physrev.00012.2014. https://pubmed.ncbi.nlm.nih.gov/25540137/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen