Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Anp32bem1flox/Cya
Common Name:
Anp32b-flox
Product ID:
S-CKO-13793
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Anp32b-flox
Strain ID
CKOCMP-67628-Anp32b-B6J-VA
Gene Name
Anp32b
Product ID
S-CKO-13793
Gene Alias
2410015B15Rik; PAL31; PHAPI2a; Ssp29
Background
C57BL/6JCya
NCBI ID
67628
Modification
Conditional knockout
Chromosome
4
Phenotype
MGI:1914878
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Anp32bem1flox/Cya mice (Catalog S-CKO-13793) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000102926
NCBI RefSeq
NM_130889
Target Region
Exon 4
Size of Effective Region
~1.3 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Anp32b, also known as acidic leucine-rich nuclear phosphoprotein 32B, is involved in multiple biological processes. It can form complexes with various proteins to regulate important cellular functions. For example, it participates in pathways related to cell development, hematopoiesis, and viral replication, and is thus of great biological importance. Genetic models, such as gene knockout (KO) or conditional knockout (CKO) mouse models, have been crucial in studying its functions [1,2,3,4].

In hematopoietic cells, conditional deletion of Anp32b impairs the repopulation capacity and post-injury regeneration of hematopoietic stem cells (HSCs). Mechanistically, Anp32b forms a repressive complex with p53 to inhibit p53's transcriptional activity, and p53 deletion can rescue the functional defect in Anp32b-deficient HSCs. In chronic myelogenous leukemia (CML), Anp32b deletion enhances p53 transcriptional activity to impair leukemic stem cell (LSC) function and shows synergistic therapeutic effects with tyrosine kinase inhibitors [1].

In B-cell acute lymphoblastic leukemia (B-ALL) mouse models, conditional deletion of Anp32b in hematopoietic cells promotes leukemogenesis. Anp32b interacts with PU.1 and enhances its transcriptional activity, and overexpression of PU.1 suppresses B-ALL progression [2].

Deletion of Anp32b in constitutive C57BL/6-derived Anp32b-/-mice leads to severe defects in ocular development. Anp32b directly interacts with paired box protein 6 (PAX6) and enhances its transcriptional activity [3].

In mice, ANP32B-/-mice infected with H3N2 or H5N1 influenza A virus (IAV) have reduced virus loads, inflammatory cytokine response, and pathogenicity compared to ANP32B + / + mice [4].

In conclusion, Anp32b plays essential roles in cell development, hematopoiesis, and viral pathogenesis. The KO/CKO mouse models have significantly contributed to understanding its functions in diseases like CML, B-ALL, and ocular developmental defects, providing potential therapeutic targets for these diseases.

References:
1. Yang, Shuo, Zhu, Xiao-Na, Zhang, Hui-Lin, Chen, Guo-Qiang, Yu, Yun. . ANP32B-mediated repression of p53 contributes to maintenance of normal and CML stem cells. In Blood, 138, 2485-2498. doi:10.1182/blood.2020010400. https://pubmed.ncbi.nlm.nih.gov/34359074/
2. Yang, Qian, Liu, Hao-Ran, Yang, Shuo, Chen, Guo-Qiang, Yu, Yun. 2023. ANP32B suppresses B-cell acute lymphoblastic leukemia through activation of PU.1 in mice. In Cancer science, 114, 2882-2894. doi:10.1111/cas.15822. https://pubmed.ncbi.nlm.nih.gov/37137487/
3. Wei, Yu-Sheng, Liu, Hao-Ran, Yang, Qian, Zhi, Zhe, Yu, Yun. 2024. Anp32b Deficiency Suppresses Ocular Development by Repression of Pax6. In Ophthalmic research, 67, 644-653. doi:10.1159/000542447. https://pubmed.ncbi.nlm.nih.gov/39504945/
4. Beck, Sebastian, Zickler, Martin, Pinho Dos Reis, Vinícius, Stanelle-Bertram, Stephanie, Gabriel, Gülşah. 2020. ANP32B Deficiency Protects Mice From Lethal Influenza A Virus Challenge by Dampening the Host Immune Response. In Frontiers in immunology, 11, 450. doi:10.3389/fimmu.2020.00450. https://pubmed.ncbi.nlm.nih.gov/32231671/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest