C57BL/6JCya-Mospd1em1flox/Cya
Common Name
Mospd1-flox
Product ID
S-CKO-14803
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-70380-Mospd1-B6J-VA
Status
When using this mouse strain in a publication, please cite “Mospd1-flox Mouse (Catalog S-CKO-14803) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Mospd1-flox
Strain ID
CKOCMP-70380-Mospd1-B6J-VA
Gene Name
Product ID
S-CKO-14803
Gene Alias
1810018L05Rik
Background
C57BL/6JCya
Gene Full Name
motile sperm domain containing 1
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr X (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000023836
NCBI Transcript ID
NM_027409
Target Region
Exon 4
Size of Effective Region
~1.9 kb
Overview of Gene Research
MOSPD1, Motile Sperm Domain-Containing Protein 1, is part of a gene family including Mospd2 and Mospd3, characterized by the major sperm protein domain and two transmembrane domains. It is located in the endoplasmic reticulum and Golgi apparatus, and can localize to the nucleus under certain conditions [2]. MOSPD1 may play roles in membrane contact sites as it can interact with FFAT-related FFNT motifs [4,5,7]. It is involved in multiple biological processes, and its dysregulation is associated with various diseases.
In breast cancer, MOSPD1 expression is significantly higher in cancer samples than normal tissues, correlating with poor clinical outcomes. MOSPD1 suppression inhibits tumor growth, while overexpression accelerates it. Silencing MOSPD1 also enhances breast cancer cell sensitivity to anti-PD-L1 therapy and decreases Th2 cell activity [1].
In gastric cancer, MOSPD1 promotes cancer progression by facilitating fatty acid metabolism through activating the MAPK pathway. MOSPD1 knockdown decreases the proliferation, migration, and invasion of gastric cancer cells [3].
In mesenchymal stem cells (MSCs), MOSPD1-null embryonic stem cells are deficient in differentiating into several cell lineages including osteoblasts, adipocytes, and hematopoietic progenitors, and the growth rate of MSC-like cells derived from MOSPD1-null ESCs is significantly impaired [6].
In colorectal cancer, MOSPD1 is upregulated by the Wnt/β-catenin signaling pathway [8].
In conclusion, MOSPD1 is crucial in multiple biological processes such as cell differentiation and proliferation. Its dysregulation significantly impacts the development and progression of various cancers, including breast, gastric, and colorectal cancer. The use of gene knockout models in mice has been instrumental in revealing these functions, providing potential therapeutic targets for these diseases.
References：
1. Jiang, Yiling, Li, Hailong, Wu, Sixuan, Du, Wei, Li, Yuehua. 2024. Deciphering MOSPD1's impact on breast cancer progression and therapeutic response. In Biology direct, 19, 88. doi:10.1186/s13062-024-00531-9. https://pubmed.ncbi.nlm.nih.gov/39369222/
2. Thaler, R, Rumpler, M, Spitzer, S, Klaushofer, K, Varga, F. . Mospd1, a new player in mesenchymal versus epidermal cell differentiation. In Journal of cellular physiology, 226, 2505-15. doi:10.1002/jcp.22595. https://pubmed.ncbi.nlm.nih.gov/21792907/
3. Wang, Chengliang, Qiu, Yunping, Zheng, Xiao, Chen, Shuhui, He, Chao. 2025. MOSPD1 facilitates fatty acid metabolism and gastric cancer progression by promoting the MAPK pathway. In Tissue & cell, 93, 102752. doi:10.1016/j.tice.2025.102752. https://pubmed.ncbi.nlm.nih.gov/39864210/
4. Cabukusta, Birol, Berlin, Ilana, van Elsland, Daphne M, van Veelen, Peter A, Neefjes, Jacques. . Human VAPome Analysis Reveals MOSPD1 and MOSPD3 as Membrane Contact Site Proteins Interacting with FFAT-Related FFNT Motifs. In Cell reports, 33, 108475. doi:10.1016/j.celrep.2020.108475. https://pubmed.ncbi.nlm.nih.gov/33296653/
5. Cabukusta, Birol, Berlin, Ilana, van Elsland, Daphne M, van Veelen, Peter A, Neefjes, Jacques. 2023. Human VAPome Analysis Reveals MOSPD1 and MOSPD3 as Membrane Contact Site Proteins Interacting with FFAT-Related FFNT Motifs. In Cell reports, 42, 112849. doi:10.1016/j.celrep.2023.112849. https://pubmed.ncbi.nlm.nih.gov/37440407/
6. Kara, Madina, Axton, Richard A, Jackson, Melany, Peault, Bruno, Forrester, Lesley M. 2015. A Role for MOSPD1 in Mesenchymal Stem Cell Proliferation and Differentiation. In Stem cells (Dayton, Ohio), 33, 3077-86. doi:10.1002/stem.2102. https://pubmed.ncbi.nlm.nih.gov/26175344/
7. James, Christina, Kehlenbach, Ralph H. 2021. The Interactome of the VAP Family of Proteins: An Overview. In Cells, 10, . doi:10.3390/cells10071780. https://pubmed.ncbi.nlm.nih.gov/34359948/
8. Horie, Chiaki, Zhu, Chi, Yamaguchi, Kiyoshi, Shida, Dai, Furukawa, Yoichi. 2022. Motile sperm domain containing 1 is upregulated by the Wnt/β-catenin signaling pathway in colorectal cancer. In Oncology letters, 24, 282. doi:10.3892/ol.2022.13402. https://pubmed.ncbi.nlm.nih.gov/35814826/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
