C57BL/6JCya-Ptges2em1flox/Cya
Common Name:
Ptges2-flox
Product ID:
S-CKO-17276
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ptges2-flox
Strain ID
CKOCMP-96979-Ptges2-B6J-VA
Gene Name
Product ID
S-CKO-17276
Gene Alias
0610038H10Rik; Gbf1; Mpges2; Pges2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ptges2em1flox/Cya mice (Catalog S-CKO-17276) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000028162
NCBI RefSeq
NM_133783
Target Region
Exon 3~7
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
Ptges2, also known as microsomal prostaglandin E synthase-2 (mPGES-2), is an enzyme involved in the synthesis of prostaglandin E2 (PGE2) from COX-derived PGH2 [5]. It can also metabolize PGH2 to malondialdehyde by forming a complex with heme. Ptges2 is associated with multiple biological pathways and is of great biological importance in processes such as systemic insulin sensitivity regulation, lipid metabolism, and inflammation-related responses [2,4]. Genetic models, like gene knockout (KO) and conditional knockout (CKO) mouse models, are valuable for studying its functions.
In AKI (acute kidney injury) models, both global and tubule-specific Ptges2-deficient mice treated with cisplatin or subjected to unilateral renal ischemia/reperfusion showed decreased renal dysfunction and morphological damage. Mechanistically, Ptges2 deficiency inhibited ferroptosis via the heme-dependent regulation of the p53/SLC7A11/GPX4 axis, indicating that blocking Ptges2 may be a promising therapeutic strategy for AKI [3]. In the context of diabetic kidney disease (DKD), global knockout or pharmacological blockage of Ptges2 attenuated diabetic podocyte injury, tubulointerstitial fibrosis, lipid accumulation, and lipotoxicity. The mechanism involves the competition between Ptges2 and Rev-Erbα for heme binding to regulate fatty acid binding protein 5 expression and lipid metabolism in the diabetic kidney, suggesting a potential treatment strategy for DKD through Ptges2 inhibition [4].
In conclusion, Ptges2 is a key enzyme in PGE2 synthesis and is involved in important biological processes. Studies using KO/CKO mouse models have revealed its role in AKI and DKD, providing potential therapeutic directions for these diseases. Additionally, it may be associated with other conditions such as basal cell carcinoma, as it was identified as a potential biomarker and therapeutic target for BCC through proteome-wide mendelian randomization, colocalization, and MR-PheWAS analyses [1].
References:
1. Han, Qiu-Ju, Zhu, Yi-Pan, Sun, Jing, Wang, Xiuyu, Zhang, Qiang-Zhe. 2024. PTGES2 and RNASET2 identified as novel potential biomarkers and therapeutic targets for basal cell carcinoma: insights from proteome-wide mendelian randomization, colocalization, and MR-PheWAS analyses. In Frontiers in pharmacology, 15, 1418560. doi:10.3389/fphar.2024.1418560. https://pubmed.ncbi.nlm.nih.gov/39035989/
2. Xiao, Ling, De Jesus, Dario F, Ju, Cheng-Wei, He, Chuan, Kulkarni, Rohit N. 2024. m6A mRNA methylation in brown fat regulates systemic insulin sensitivity via an inter-organ prostaglandin signaling axis independent of UCP1. In Cell metabolism, 36, 2207-2227.e9. doi:10.1016/j.cmet.2024.08.006. https://pubmed.ncbi.nlm.nih.gov/39255799/
3. Zhong, Dandan, Quan, Lingling, Hao, Chang, Jia, Zhanjun, Sun, Ying. 2023. Targeting mPGES-2 to protect against acute kidney injury via inhibition of ferroptosis dependent on p53. In Cell death & disease, 14, 710. doi:10.1038/s41419-023-06236-7. https://pubmed.ncbi.nlm.nih.gov/37907523/
4. Zhong, Dandan, Chen, Jingshuo, Qiao, Ranran, Jia, Zhanjun, Sun, Ying. 2024. Genetic or pharmacologic blockade of mPGES-2 attenuates renal lipotoxicity and diabetic kidney disease by targeting Rev-Erbα/FABP5 signaling. In Cell reports, 43, 114075. doi:10.1016/j.celrep.2024.114075. https://pubmed.ncbi.nlm.nih.gov/38583151/
5. Nakatani, Yoshihito, Kudo, Ichiro. . [Prostaglandin E2 synthases]. In Nihon yakurigaku zasshi. Folia pharmacologica Japonica, 120, 373-8. doi:. https://pubmed.ncbi.nlm.nih.gov/12528468/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen