C57BL/6JCya-Lbrem1flox/Cya
Common Name:
Lbr-flox
Product ID:
S-CKO-17315
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Lbr-flox
Strain ID
CKOCMP-98386-Lbr-B6J-VA
Gene Name
Product ID
S-CKO-17315
Gene Alias
C14SR; ic
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lbrem1flox/Cya mice (Catalog S-CKO-17315) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000005003
NCBI RefSeq
NM_133815
Target Region
Exon 2
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Lbr, short for Lamin B receptor, is a nuclear-membrane protein with sterol reductase activity. It resides in the inner nuclear membrane, providing attachment sites for chromatin and the nuclear lamina, thus being crucial for the functional organization of the nucleus. It is also involved in regulating heterochromatin organization [1,2].
A 236bp N-terminal deletion in the mouse Lbr gene, generated via CRISPR/Cas-9 gene editing, recapitulates human Pelger-Huet anomaly (PHA) phenotypes better than existing models, and shows minor X chromosome inactivation defects without overt in vivo phenotypes. This model provides a valuable tool for understanding PHA etiology [1]. Additionally, knockdown of LBR in human cells facilitated the induction of cellular senescence by excess thymidine in cancerous HeLa cells and normal human diploid fibroblast TIG-7 cells, suggesting its role in preventing cellular senescence [3].
In conclusion, Lbr is important for nuclear organization and heterochromatin regulation. The gene-edited mouse model for Lbr has significantly contributed to understanding PHA. Lbr also plays a role in suppressing cellular senescence, which helps in further exploring the mechanisms related to cell growth and specific blood disorders.
References:
1. Young, Alexander Neil, Perlas, Emerald, Ruiz-Blanes, Nerea, Giannakouros, Thomas, Cerase, Andrea. 2021. Deletion of LBR N-terminal domains recapitulates Pelger-Huet anomaly phenotypes in mouse without disrupting X chromosome inactivation. In Communications biology, 4, 478. doi:10.1038/s42003-021-01944-2. https://pubmed.ncbi.nlm.nih.gov/33846535/
2. Chu, A, Rassadi, R, Stochaj, U. . Velcro in the nuclear envelope: LBR and LAPs. In FEBS letters, 441, 165-9. doi:. https://pubmed.ncbi.nlm.nih.gov/9883877/
3. Arai, Rumi, En, Atsuki, Takauji, Yuki, Fujii, Michihiko, Ayusawa, Dai. 2019. Lamin B receptor (LBR) is involved in the induction of cellular senescence in human cells. In Mechanisms of ageing and development, 178, 25-32. doi:10.1016/j.mad.2019.01.001. https://pubmed.ncbi.nlm.nih.gov/30615890/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen