C57BL/6JCya-Cldn7em1flox/Cya
Common Name:
Cldn7-flox
Product ID:
S-CKO-17624
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Cldn7-flox
Strain ID
CKOCMP-53624-Cldn7-B6J-VB
Gene Name
Product ID
S-CKO-17624
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cldn7em1flox/Cya mice (Catalog S-CKO-17624) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000108597
NCBI RefSeq
NM_001193619
Target Region
Exon 3~5
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Cldn7, also known as claudin 7, is a major component of tight junctions (TJs) [2,4,5,6,7]. TJs play a crucial role in maintaining cell-cell adhesion, and Cldn7 is involved in various biological processes related to cell-cell interactions. It is associated with pathways like the regulation of cell proliferation, invasion, and epithelial-mesenchymal transition (EMT) [1,3,5,6,7].
In colorectal cancer, in vitro and in vivo assays using p53 wild-type CRC cells showed that Cldn7, which is regulated by p53, inhibits cell proliferation [1]. High Cldn7 expression was negatively correlated with tumor size, invasion depth, lymphatic metastasis, and AJCC III/IV stage, and positively associated with favorable prognosis [1].
In breast cancer, database and clinical sample validation indicated that Cldn7 was significantly overexpressed and its overexpression was correlated with poor DFS [3]. TIMER2.0 analysis showed its overexpression was negatively associated with the activation of B-cells, CD4+ T-cells, and CD8+ T-cells, but positively with M0 macrophages [3].
In gastric cancer, Cldn7 was overexpressed, and it promoted cancer cell proliferation, invasion, and EMT [6].
In clear cell renal cell carcinoma (ccRCC), downregulation of Cldn7 due to promoter hypermethylation was associated with cancer progression and poor prognosis, and overexpression of Cldn7 induced cell apoptosis, suppressed proliferation, migration, and invasion of ccRCC cells in vitro and in vivo [7].
In murine breast cancer models, Cldn7 knockdown in organoids induced smooth muscle actin (SMA)-related genes and a mesenchymal phenotype, and Cldn7 was shown to suppress breast cancer invasion and metastasis through negative regulation of SMA-related and mesenchymal gene expression [8].
In conclusion, Cldn7 is a key component of tight junctions that significantly impacts the development and progression of multiple cancers, including colorectal, breast, gastric, and clear cell renal cell carcinomas, as well as breast cancer metastasis. Studies using in vitro and in vivo models, including gene-related functional studies in cancer cells, have revealed its role in regulating cell proliferation, invasion, apoptosis, and EMT, highlighting its potential as a biomarker and therapeutic target in these disease areas.
References：
1. Hou, Yichao, Hou, Lidan, Liang, Yu, Fang, Jingyuan, Meng, Xiangjun. 2020. The p53-inducible CLDN7 regulates colorectal tumorigenesis and has prognostic significance. In Neoplasia (New York, N.Y.), 22, 590-603. doi:10.1016/j.neo.2020.09.001. https://pubmed.ncbi.nlm.nih.gov/32992138/
2. Yang, Hui-Li, Lai, Zhen-Zhen, Shi, Jia-Wei, Li, Da-Jin, Li, Ming-Qing. 2022. A defective lysophosphatidic acid-autophagy axis increases miscarriage risk by restricting decidual macrophage residence. In Autophagy, 18, 2459-2480. doi:10.1080/15548627.2022.2039000. https://pubmed.ncbi.nlm.nih.gov/35220880/
3. Fan, Xiaojie, Qi, Aifeng, Zhang, Meng, Han, Dandan, Liu, Yueping. 2024. Expression and clinical significance of CLDN7 and its immune-related cells in breast cancer. In Diagnostic pathology, 19, 113. doi:10.1186/s13000-024-01513-1. https://pubmed.ncbi.nlm.nih.gov/39175074/
4. Marincola Smith, Paula, Choksi, Yash A, Markham, Nicholas O, Means, Anna L, Beauchamp, R Daniel. 2021. Colon epithelial cell TGFβ signaling modulates the expression of tight junction proteins and barrier function in mice. In American journal of physiology. Gastrointestinal and liver physiology, 320, G936-G957. doi:10.1152/ajpgi.00053.2021. https://pubmed.ncbi.nlm.nih.gov/33759564/
5. Wang, Wentao, Zhou, Yi, Li, Wei, Quan, Chengshi, Li, Yanru. 2024. Claudins and hepatocellular carcinoma. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 171, 116109. doi:10.1016/j.biopha.2023.116109. https://pubmed.ncbi.nlm.nih.gov/38185042/
6. Wu, Z, Shi, J, Song, Y, Gao, P, Wang, Z. . Claudin-7 (CLDN7) is overexpressed in gastric cancer and promotes gastric cancer cell proliferation, invasion and maintains mesenchymal state. In Neoplasma, 65, 349-359. doi:10.4149/neo_2018_170320N200. https://pubmed.ncbi.nlm.nih.gov/29788731/
7. Li, Yifan, Gong, Yanqing, Ning, Xianghui, Li, Xuesong, Zhou, Liqun. 2018. Downregulation of CLDN7 due to promoter hypermethylation is associated with human clear cell renal cell carcinoma progression and poor prognosis. In Journal of experimental & clinical cancer research : CR, 37, 276. doi:10.1186/s13046-018-0924-y. https://pubmed.ncbi.nlm.nih.gov/30428910/
8. West, Junior J, Golloshi, Rosela, Cho, Chae Yun, Fertig, Elana J, Ewald, Andrew J. 2024. Claudin 7 suppresses invasion and metastasis through repression of a smooth muscle actin program. In The Journal of cell biology, 223, . doi:10.1083/jcb.202311002. https://pubmed.ncbi.nlm.nih.gov/39320351/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen