C57BL/6JCya-Trap1em1flox/Cya
Common Name:
Trap1-flox
Product ID:
S-CKO-17886
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Trap1-flox
Strain ID
CKOCMP-68015-Trap1-B6J-VB
Gene Name
Product ID
S-CKO-17886
Gene Alias
2410002K23Rik; HSP75
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Trap1em1flox/Cya mice (Catalog S-CKO-17886) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000006137
NCBI RefSeq
NM_026508
Target Region
Exon 2~3
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Trap1, also known as Tumour necrosis factor receptor-associated protein 1 and Heat shock protein 75 (HSP75), is a member of the heat shock protein 90 (HSP90) chaperone family mainly residing in the mitochondria [2]. As a mitochondrial molecular chaperone, it supports protein folding, maintains mitochondrial integrity, and regulates mitochondrial bioenergetics, redox homeostasis, oxidative stress-induced cell death, apoptosis, and unfolded protein response (UPR) in the endoplasmic reticulum (ER) [2]. It is also involved in metabolic reprogramming, such as promoting the switch from oxidative phosphorylation to glycolysis [4].
In VSMC-specific Trap1 knockout ApoeKO mice (ApoeKOTrap1SMCKO), VSMC senescence and atherosclerosis were mitigated, suggesting that Trap1 drives VSMC senescence and promotes atherosclerosis via metabolic reprogramming. Mechanistically, Trap1 increases aerobic glycolysis, leading to elevated lactate production, which promotes histone H4 lysine 12 lactylation (H4K12la) by down-regulating HDAC3, and H4K12la activates SASP transcription to exacerbate VSMC senescence [1]. In ischemic retinopathy mouse models, genetic Trap1 ablation alleviates retinal pathologies via proteolytic HIF1α degradation, indicating that Trap1 is essential for blood-retinal barrier (BRB) breakdown and pathologic retinal neovascularization [3]. In primary cardiomyocytes under high glucose/palmitate conditions, Trap1 inhibits MARCH5-mediated MIC60 degradation, thereby alleviating mitochondrial dysfunction and apoptosis [5].
In conclusion, Trap1 plays crucial roles in maintaining mitochondrial function, metabolic regulation, and in multiple disease-related processes. The gene knockout and conditional knockout mouse models have significantly contributed to understanding Trap1's role in atherosclerosis, ischemic retinopathy, and diabetic cardiomyopathy, providing insights into potential therapeutic targets for these diseases.
References:
1. Li, Xuesong, Chen, Minghong, Chen, Xiang, Ji, Yong, Chen, Hongshan. . TRAP1 drives smooth muscle cell senescence and promotes atherosclerosis via HDAC3-primed histone H4 lysine 12 lactylation. In European heart journal, 45, 4219-4235. doi:10.1093/eurheartj/ehae379. https://pubmed.ncbi.nlm.nih.gov/39088352/
2. Ramos Rego, Inês, Santos Cruz, Beatriz, Ambrósio, António Francisco, Alves, Celso Henrique. 2021. TRAP1 in Oxidative Stress and Neurodegeneration. In Antioxidants (Basel, Switzerland), 10, . doi:10.3390/antiox10111829. https://pubmed.ncbi.nlm.nih.gov/34829705/
3. Kim, So-Yeon, Yoon, Nam Gu, Im, Jin Young, Park, Dong Ho, Kang, Byoung Heon. 2023. Targeting the Mitochondrial Chaperone TRAP1 Alleviates Vascular Pathologies in Ischemic Retinopathy. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2302776. doi:10.1002/advs.202302776. https://pubmed.ncbi.nlm.nih.gov/37983591/
4. Dekker, Françoise A, Rüdiger, Stefan G D. 2021. The Mitochondrial Hsp90 TRAP1 and Alzheimer's Disease. In Frontiers in molecular biosciences, 8, 697913. doi:10.3389/fmolb.2021.697913. https://pubmed.ncbi.nlm.nih.gov/34222342/
5. Zhang, Lingxiao, Luo, Yuanyuan, Lv, Linyan, Liu, Guihua, Zhao, Tongfeng. 2023. TRAP1 inhibits MARCH5-mediated MIC60 degradation to alleviate mitochondrial dysfunction and apoptosis of cardiomyocytes under diabetic conditions. In Cell death and differentiation, 30, 2336-2350. doi:10.1038/s41418-023-01218-w. https://pubmed.ncbi.nlm.nih.gov/37679468/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen