C57BL/6JCya-Lmbrd1em1flox/Cya
Common Name:
Lmbrd1-flox
Product ID:
S-CKO-18110
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Lmbrd1-flox
Strain ID
CKOCMP-68421-Lmbrd1-B6J-VB
Gene Name
Product ID
S-CKO-18110
Gene Alias
0910001K20Rik; LMBD1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lmbrd1em1flox/Cya mice (Catalog S-CKO-18110) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000191471
NCBI RefSeq
NM_026719
Target Region
Exon 3
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Lmbrd1, also known as limb region 1 (LMBR1) domain containing 1 gene, encodes a lysosomal membrane protein LMBD1. It is crucial for vitamin B₁₂ metabolism, specifically facilitating lysosomal cobalamin export. Mutations in Lmbrd1 lead to the cblF defect, disrupting cobalamin coenzyme synthesis and causing disorders related to methionine and methylmalonyl-coenzyme A metabolism [1,4].
Lmbrd1-deficient mice generated using the Cre/LoxP system showed that complete loss of Lmbrd1 function leads to early embryonic death. Studies on these mice revealed that the initial formation of the proximal-distal axis is unaffected, but the initiation of gastrulation is disturbed [2]. Heterozygous knockout mice (Lmbrd1+/-) exhibited increased cardiac-glucose uptake, cardiac hypertrophy, ventricular tissue fibrosis, elevated heart rate, and enhanced cardiac muscle contractility. As they aged, signs of ventricular function deterioration were observed, suggesting Lmbrd1's role in mediating cardiac energy homeostasis and regulating cardiac function [3].
In conclusion, Lmbrd1 is essential for processes like gastrulation during embryogenesis and for maintaining normal cardiac function. Studies using gene-knockout mouse models have provided insights into its role in these biological processes and the associated disease conditions such as cobalamin-related disorders and cardiac diseases.
References:
1. Rutsch, Frank, Gailus, Susann, Suormala, Terttu, Fowler, Brian. 2010. LMBRD1: the gene for the cblF defect of vitamin B₁₂ metabolism. In Journal of inherited metabolic disease, 34, 121-6. doi:10.1007/s10545-010-9083-9. https://pubmed.ncbi.nlm.nih.gov/20446115/
2. Buers, Insa, Pennekamp, Petra, Nitschke, Yvonne, Skryabin, Boris V, Rutsch, Frank. 2016. Lmbrd1 expression is essential for the initiation of gastrulation. In Journal of cellular and molecular medicine, 20, 1523-33. doi:10.1111/jcmm.12844. https://pubmed.ncbi.nlm.nih.gov/27061115/
3. Tseng, Linda Tzu-Ling, Lin, Chieh-Liang, Pan, Kuei-Hsiang, Chang, Shin C, Chang, Ming-Fu. 2017. Single allele Lmbrd1 knockout results in cardiac hypertrophy. In Journal of the Formosan Medical Association = Taiwan yi zhi, 117, 471-479. doi:10.1016/j.jfma.2017.05.002. https://pubmed.ncbi.nlm.nih.gov/28549591/
4. Gailus, Susann, Suormala, Terttu, Malerczyk-Aktas, Ayse Gül, Hennermann, Julia B, Rutsch, Frank. 2010. A novel mutation in LMBRD1 causes the cblF defect of vitamin B(12) metabolism in a Turkish patient. In Journal of inherited metabolic disease, 33, 17-24. doi:10.1007/s10545-009-9032-7. https://pubmed.ncbi.nlm.nih.gov/20127417/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen