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C57BL/6JCya-Flt3lem1flox/Cya
Common Name:
Flt3l-flox
Product ID:
S-CKO-18135
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Flt3l-flox
Strain ID
CKOCMP-14256-Flt3l-B6J-VA
Gene Name
Flt3l
Product ID
S-CKO-18135
Gene Alias
Flt3lg; Ly72L
Background
C57BL/6JCya
NCBI ID
14256
Modification
Conditional knockout
Chromosome
7
Phenotype
MGI:95560
Document
Click here to download >>
Application
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Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Flt3lem1flox/Cya mice (Catalog S-CKO-18135) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000146760
NCBI RefSeq
NM_013520
Target Region
Exon 5~6
Size of Effective Region
~1.9 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Flt3l, encoded by FLT3LG, is a hematopoietic factor also known as FMS-related tyrosine kinase 3 ligand. It acts on Flt3-expressing multipotent progenitors and common lymphoid progenitors. The Flt3/Flt3L axis is mainly involved in the generation of conventional DCs (cDCs) and plasmacytoid DCs (pDCs), and Flt3 signaling through PI3K and mTOR may affect the function of mature DCs [2].

In mice, Flt3l is essential for the development of natural killer (NK) cells, B cells, and dendritic cells (DCs) [1]. In humans, three individuals homozygous for a loss-of-function FLT3LG variant had hypoplastic bone marrow, low levels of hematopoietic progenitors, and reduced counts of B cells, monocytes, and DCs in the blood [1]. Maternal low-fiber diet in mice led to reduced neonatal intestinal epithelial cell secretion of Flt3L, impairing pDC hematopoiesis and increasing LRI severity in offspring [3].

In cancer research, in vitro experiments showed that the combination of Flt3L, LIF, and IL-10 promoted the selective development of ESAMlow cDC2B from murine bone marrow [5]. Also, Flt3L therapy increased all cDC subsets in orthotopic breast and lung cancer models in mice, but did not reduce tumor growth [4].

In conclusion, Flt3l is crucial for the development of multiple hematopoietic lineages, including B cells and DCs in both mice and humans. In mice, it also impacts NK cell development. Its role in diseases, especially in relation to immune responses in cancer and infectious diseases, has been revealed through various mouse models. Understanding Flt3l can provide insights into hematopoietic development and potential immunotherapy strategies.

References:
1. Momenilandi, Mana, Lévy, Romain, Sobrino, Steicy, Casanova, Jean-Laurent, Béziat, Vivien. 2024. FLT3L governs the development of partially overlapping hematopoietic lineages in humans and mice. In Cell, 187, 2817-2837.e31. doi:10.1016/j.cell.2024.04.009. https://pubmed.ncbi.nlm.nih.gov/38701783/
2. Cueto, Francisco J, Sancho, David. 2021. The Flt3L/Flt3 Axis in Dendritic Cell Biology and Cancer Immunotherapy. In Cancers, 13, . doi:10.3390/cancers13071525. https://pubmed.ncbi.nlm.nih.gov/33810248/
3. Sikder, Md Al Amin, Rashid, Ridwan B, Ahmed, Tufael, Dennis, Paul G, Phipps, Simon. 2023. Maternal diet modulates the infant microbiome and intestinal Flt3L necessary for dendritic cell development and immunity to respiratory infection. In Immunity, 56, 1098-1114.e10. doi:10.1016/j.immuni.2023.03.002. https://pubmed.ncbi.nlm.nih.gov/37003256/
4. Clappaert, Emile J, Kancheva, Daliya, Brughmans, Jan, Deschoemaeker, Sofie, Laoui, Damya. 2023. Flt3L therapy increases the abundance of Treg-promoting CCR7+ cDCs in preclinical cancer models. In Frontiers in immunology, 14, 1166180. doi:10.3389/fimmu.2023.1166180. https://pubmed.ncbi.nlm.nih.gov/37622122/
5. Cyran, Laura, Serfling, Julia, Kirschner, Luisa, Bodem, Jochen, Lutz, Manfred B. 2022. Flt3L, LIF, and IL-10 combination promotes the selective in vitro development of ESAMlow cDC2B from murine bone marrow. In European journal of immunology, 52, 1946-1960. doi:10.1002/eji.202149663. https://pubmed.ncbi.nlm.nih.gov/35357005/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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