C57BL/6JCya-Tle3em1flox/Cya
Common Name:
Tle3-flox
Product ID:
S-CKO-18457
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Tle3-flox
Strain ID
CKOCMP-21887-Tle3-B6J-VB
Gene Name
Product ID
S-CKO-18457
Gene Alias
2610103N05Rik; ESG; Grg3a; Grg3b; mKIAA1547
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tle3em1flox/Cya mice (Catalog S-CKO-18457) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000160882
NCBI RefSeq
NM_001083927
Target Region
Exon 3~4
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Tle3, short for Transducin-like enhancer of split 3, is a transcriptional co-factor and corepressor that plays a crucial role in multiple biological processes. It is involved in various signaling pathways and is important for maintaining cell lineage identity, immune homeostasis, and has implications in cancer development and metabolism [1-10]. Genetic models, such as KO/CKO mouse models, have been valuable in studying its functions.
In CD8+ T cells, genetic ablation of Tle3 promoted CD8+ TCM cell formation at the expense of CD8+ TEM cells, indicating its role in controlling T cell fates [1]. In luminal breast cancer, TLE3 actively repressed the gene-expression signature of basal-like breast cancers, preventing a hybrid epithelial-mesenchymal state and reducing metastasis [2]. In myeloid cells lacking Tle3, there was an increase in regulatory T and TH17 cells, affecting intestinal immune homeostasis [3]. Loss of Tle3 in prostate cancer cells conferred resistance to AR inhibitors [4]. In mesenchymal stem cells, adipogenic TLE3 is selectively removed by chaperone-mediated autophagy to promote osteogenesis [5]. In colorectal cancer, down-regulation of TLE3 was associated with poorer patient survival, and overexpression of TLE3 repressed cancer cell proliferation by inhibiting MAPK and AKT signaling pathways [6]. In beige adipocytes, conditional deletion of TLE3 promoted mitochondrial oxidative metabolism and improved glucose control [7].
In conclusion, Tle3 is essential for maintaining cell lineage fidelity in T cells and breast cancer cells, regulating immune homeostasis in the intestine, influencing prostate cancer response to treatment, and playing roles in mesenchymal stem cell differentiation, colorectal cancer progression, and beige adipocyte-related metabolism. The KO/CKO mouse models have significantly contributed to understanding Tle3's role in these disease-related biological processes, providing insights for potential therapeutic strategies.
References:
1. Zhao, Xin, Hu, Wei, Park, Sung Rye, Shan, Qiang, Xue, Hai-Hui. 2024. The transcriptional cofactor Tle3 reciprocally controls effector and central memory CD8+ T cell fates. In Nature immunology, 25, 294-306. doi:10.1038/s41590-023-01720-w. https://pubmed.ncbi.nlm.nih.gov/38238608/
2. Anstine, Lindsey J, Majmudar, Parth R, Aponte, Amy, Thompson, Cheryl L, Keri, Ruth A. . TLE3 Sustains Luminal Breast Cancer Lineage Fidelity to Suppress Metastasis. In Cancer research, 83, 997-1015. doi:10.1158/0008-5472.CAN-22-3133. https://pubmed.ncbi.nlm.nih.gov/36696357/
3. Li, Xiaoyu, Zhang, Bin, Zhang, Xiang, Xue, Hai-Hui, Hu, Xiaoyu. 2023. TLE3 and TLE4-coordinated colonic macrophage-CD4+ T cell crosstalk maintains intestinal immune homeostasis. In Mucosal immunology, 16, 50-60. doi:10.1016/j.mucimm.2022.12.005. https://pubmed.ncbi.nlm.nih.gov/36801171/
4. Palit, Sander Al, Vis, Daniel, Stelloo, Suzan, Zwart, Wilbert, van der Heijden, Michiel S. 2019. TLE3 loss confers AR inhibitor resistance by facilitating GR-mediated human prostate cancer cell growth. In eLife, 8, . doi:10.7554/eLife.47430. https://pubmed.ncbi.nlm.nih.gov/31855178/
5. Gong, Yan, Li, Ziqi, Zou, Shitian, Bai, Xiaochun, Zou, Zhipeng. 2021. Vangl2 limits chaperone-mediated autophagy to balance osteogenic differentiation in mesenchymal stem cells. In Developmental cell, 56, 2103-2120.e9. doi:10.1016/j.devcel.2021.06.011. https://pubmed.ncbi.nlm.nih.gov/34214490/
6. Yang, Run-Wei, Zeng, Ying-Yue, Wei, Wen-Ting, Ding, Yan-Qing, Liao, Wen-Ting. 2016. TLE3 represses colorectal cancer proliferation by inhibiting MAPK and AKT signaling pathways. In Journal of experimental & clinical cancer research : CR, 35, 152. doi:. https://pubmed.ncbi.nlm.nih.gov/27669982/
7. Pearson, Stephanie, Loft, Anne, Rajbhandari, Prashant, Mandrup, Susanne, Villanueva, Claudio J. 2019. Loss of TLE3 promotes the mitochondrial program in beige adipocytes and improves glucose metabolism. In Genes & development, 33, 747-762. doi:10.1101/gad.321059.118. https://pubmed.ncbi.nlm.nih.gov/31123067/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen