C57BL/6JCya-Ing3em1flox/Cya
Common Name:
Ing3-flox
Product ID:
S-CKO-18766
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ing3-flox
Strain ID
CKOCMP-71777-Ing3-B6J-VB
Gene Name
Product ID
S-CKO-18766
Gene Alias
1300013A07Rik; P47ING3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ing3em1flox/Cya mice (Catalog S-CKO-18766) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031680
NCBI RefSeq
NM_023626
Target Region
Exon 3
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
ING3, a member of the Inhibitor of Growth (ING) family, is a histone-modifying protein involved in regulating cell proliferation, senescence, apoptosis, chromatin remodeling, and DNA repair [3,7]. It is a stoichiometric component of the NuA4-Tip60 MYST histone acetyl-transferase complex, which is responsible for histone H2A and H4 acetylation [3]. ING3 also plays a role in the DNA damage response pathway by being required for ATM activation and subsequent DNA repair [5].
In various cancers, ING3 shows different roles. In lung adenocarcinoma, down-regulated ING3 expression is observed, and up-regulation of ING3 inhibits cell proliferation, migration, invasion, and induces cell cycle arrest by negatively regulating ITGB4 expression to inactivate Src/FAK signaling [2]. In breast cancer, nuclear ING3 expression is reduced, and its down-regulation is correlated with poor prognosis, and overexpression of ING3 inhibits cell migration and invasion [1,4]. However, in prostate cancer, ING3 promotes cancer growth by activating the androgen receptor, and high levels of ING3 predict shorter patient survival in a low AR subgroup [6]. In a transgenic mouse model with insertional mutation of Ing3, homozygous mutants are embryonically lethal, showing growth retardation and severe developmental disorders, especially in neural tube closure and primary brain vesicle formation, indicating ING3 is essential for normal embryonic development [7].
In conclusion, ING3 has diverse functions in different biological processes and disease conditions. It plays crucial roles in embryonic development, and its dysregulation is associated with cancer progression. The use of mouse models, such as the Ing3-disrupted transgenic mouse, has been instrumental in understanding its role in embryonic development and in revealing its dual nature as a potential tumor suppressor or oncogene in different cancers.
References:
1. Wu, Xiaoyan, Chen, Chuang, Luo, Bin, Wu, Hao, Yuan, Jingping. 2021. Nuclear ING3 Expression Is Correlated With a Good Prognosis of Breast Cancer. In Frontiers in oncology, 10, 589009. doi:10.3389/fonc.2020.589009. https://pubmed.ncbi.nlm.nih.gov/33469513/
2. Cheng, Shiliang, Li, Meng, Zheng, Wen, Zhuo, Jinhua, Zhang, Lu. 2024. ING3 inhibits the malignant progression of lung adenocarcinoma by negatively regulating ITGB4 expression to inactivate Src/FAK signaling. In Cellular signalling, 117, 111066. doi:10.1016/j.cellsig.2024.111066. https://pubmed.ncbi.nlm.nih.gov/38281617/
3. Ferreras-Gutiérrez, Mariola, Chaves-Arquero, Belén, González-Magaña, Amaia, Medrano, Francisco J, Blanco, Francisco J. 2023. Structural analysis of ING3 protein and histone H3 binding. In International journal of biological macromolecules, 242, 124724. doi:10.1016/j.ijbiomac.2023.124724. https://pubmed.ncbi.nlm.nih.gov/37148949/
4. Li, Huimeng, Zhang, Hengyu, Tan, Xin, Liu, Rui, Tang, Shicong. 2021. Overexpression of ING3 is associated with attenuation of migration and invasion in breast cancer. In Experimental and therapeutic medicine, 22, 699. doi:10.3892/etm.2021.10131. https://pubmed.ncbi.nlm.nih.gov/34007308/
5. Mouche, Audrey, Archambeau, Jérôme, Ricordel, Charles, Grenon, Muriel, Pedeux, Rémy. 2019. ING3 is required for ATM signaling and DNA repair in response to DNA double strand breaks. In Cell death and differentiation, 26, 2344-2357. doi:10.1038/s41418-019-0305-x. https://pubmed.ncbi.nlm.nih.gov/30804473/
6. Nabbi, Arash, McClurg, Urszula L, Thalappilly, Subhash, Binda, Olivier, Riabowol, Karl T. 2017. ING3 promotes prostate cancer growth by activating the androgen receptor. In BMC medicine, 15, 103. doi:10.1186/s12916-017-0854-0. https://pubmed.ncbi.nlm.nih.gov/28511652/
7. Fink, Dieter, Yau, Tienyin, Nabbi, Arash, Riabowol, Karl, Rülicke, Thomas. 2019. Loss of Ing3 Expression Results in Growth Retardation and Embryonic Death. In Cancers, 12, . doi:10.3390/cancers12010080. https://pubmed.ncbi.nlm.nih.gov/31905726/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen