C57BL/6JCya-Nup35em1flox/Cya
Common Name:
Nup35-flox
Product ID:
S-CKO-19186
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Nup35-flox
Strain ID
CKOCMP-69482-Nup35-B6J-VB
Gene Name
Product ID
S-CKO-19186
Gene Alias
2310006I24Rik; 35kDa; 5330402E05Rik; MP44; NO44
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nup35em1flox/Cya mice (Catalog S-CKO-19186) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000028382
NCBI RefSeq
NM_027091
Target Region
Exon 3
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Nup35, also known as Nup53 in vertebrates, is a nucleoporin that is part of the nuclear pore complex (NPC). NPCs are gateways for transport between the nucleus and cytoplasm in eukaryotic cells, and Nup35 plays crucial roles in various biological processes such as nuclear transport, gene expression regulation, and nuclear envelope assembly [2]. It has been associated with pathways related to cell division, pH homeostasis, and viral entry [1,3,4]. Genetic models, especially mouse models, have been valuable in studying its functions.
In Caenorhabditis elegans, depletion of Nup35/NPP-19 inhibits NE localization of Nup155/NPP-8, NPC assembly, and nuclear lamina formation, leading to chromosome missegregation, nuclear morphology defects, and embryonic death around mid-gastrulation [2]. In mice, a point mutation in the RNA recognition motif of Nup35 causes a degenerative myopathy specifically affecting colonic smooth muscle, resulting in a severe megacolon and reduced lifespan, which may serve as a model for a subtype of chronic intestinal pseudo-obstruction (CIPO) [5]. In cardiomyocytes, Nup35 ablation weakens the resistance to an acid challenge by depressing the expression of Na⁺-H⁺ exchanger-1 (NHE1), as Nup35 regulates NHE1 expression by controlling the nucleo-cytoplasmic trafficking of nhe1 mRNA [3]. In oocytes, knockdown of Nup35 significantly compromises the extrusion of the first polar body, with defects in spindle assembly and chromosome alignment [4].
In conclusion, Nup35 is essential for nuclear assembly, cell division processes in embryos and oocytes, and pH homeostasis in cardiomyocytes. The study of Nup35 knockout or mutant mouse models has provided insights into its role in diseases such as CIPO and potential mechanisms underlying ischemic cardiac diseases, highlighting its significance in understanding biological functions and disease mechanisms [2,3,5].
References:
1. Xue, Guangai, Yu, Hyun Jae, Buffone, Cindy, Diaz-Griffero, Felipe, KewalRamani, Vineet N. 2023. The HIV-1 capsid core is an opportunistic nuclear import receptor. In Nature communications, 14, 3782. doi:10.1038/s41467-023-39146-5. https://pubmed.ncbi.nlm.nih.gov/37355754/
2. Ródenas, Eduardo, Klerkx, Elke P F, Ayuso, Cristina, Audhya, Anjon, Askjaer, Peter. 2008. Early embryonic requirement for nucleoporin Nup35/NPP-19 in nuclear assembly. In Developmental biology, 327, 399-409. doi:10.1016/j.ydbio.2008.12.024. https://pubmed.ncbi.nlm.nih.gov/19146848/
3. Xu, Liang, Pan, Lei, Li, Jun, Peng, Luying, Chen, Yi-Han. 2015. Nucleoporin 35 regulates cardiomyocyte pH homeostasis by controlling Na+-H+ exchanger-1 expression. In Journal of molecular cell biology, 7, 476-85. doi:10.1093/jmcb/mjv054. https://pubmed.ncbi.nlm.nih.gov/26260029/
4. Chen, Fan, Jiao, Xiao-Fei, Zhang, Jia-Yu, Miao, Yi-Liang, Huo, Li-Jun. 2018. Nucleoporin35 is a novel microtubule associated protein functioning in oocyte meiotic spindle architecture. In Experimental cell research, 371, 435-443. doi:10.1016/j.yexcr.2018.09.004. https://pubmed.ncbi.nlm.nih.gov/30195030/
5. Parish, Ian A, Stamp, Lincon A, Lorenzo, Ayla May D, Young, Heather M, Furness, John B. 2016. A Novel Mutation in Nucleoporin 35 Causes Murine Degenerative Colonic Smooth Muscle Myopathy. In The American journal of pathology, 186, 2254-61. doi:10.1016/j.ajpath.2016.04.016. https://pubmed.ncbi.nlm.nih.gov/27427419/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen