C57BL/6JCya-Popdc3em1flox/Cya
Common Name
Popdc3-flox
Product ID
S-CKO-19211
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-78977-Popdc3-B6J-VB
Status
When using this mouse strain in a publication, please cite “Popdc3-flox Mouse (Catalog S-CKO-19211) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Popdc3-flox
Strain ID
CKOCMP-78977-Popdc3-B6J-VB
Gene Name
Product ID
S-CKO-19211
Gene Alias
Pop3
Background
C57BL/6JCya
Gene Full Name
popeye domain containing 3
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 10 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000019994
NCBI Transcript ID
NM_024286
Target Region
Exon 2
Size of Effective Region
~1.4 kb
Overview of Gene Research
Popdc3, a transmembrane protein, contains a unique cyclic adenosine monophosphate (cAMP) binding site. It is widely expressed in mammalian tissues, with the highest levels in skeletal muscle. POPDC3 is involved in cAMP signaling and plays a key role in many physiological and pathological processes. It is considered a candidate biomarker and potential therapeutic target for cancer. Additionally, its gene variants have been associated with limb-girdle muscular dystrophy (LGMD) type 26 [1,2,3,6].
Functional analyses in zebrafish popdc3 knockdown models showed that knockdown of popdc3 resulted in larvae with tail curling and dystrophic muscle features, indicating its importance for skeletal muscle function [2]. In Xenopus laevis oocytes, mutant POPDC3 proteins caused aberrant modulation of the mechano-gated potassium channel, TREK-1 [2]. In head and neck squamous cell carcinoma (HNSCC), high POPDC3 expression correlated with poor prognosis and radioresistance [4]. In gastric cancer, reduced Popdc3 expression was associated with depth of invasion, lymph node metastasis, and poor survival [5].
In conclusion, POPDC3 is crucial for skeletal muscle function and is implicated in cancer-related processes such as prognosis and radioresistance in HNSCC, as well as carcinogenesis and progression in gastric cancer. The use of knockdown models in zebrafish and functional studies in Xenopus oocytes have revealed its role in muscle function and ion channel modulation. These findings highlight the significance of POPDC3 in both normal biological functions and disease conditions [1,2,4,5].
References：
1. Sun, Chen-Chen, Zhao, Zhe, Peng, Xi-Yang, Zheng, Lan, Tang, Chang-Fa. 2023. Progress on the study of Popeye domain-containing 3 (POPDC3) in malignancies and striated muscle function and homeostasis. In Clinical genetics, 103, 617-624. doi:10.1111/cge.14318. https://pubmed.ncbi.nlm.nih.gov/36843357/
2. Vissing, John, Johnson, Katherine, Töpf, Ana, Brand, Thomas, Straub, Volker. 2019. POPDC3 Gene Variants Associate with a New Form of Limb Girdle Muscular Dystrophy. In Annals of neurology, 86, 832-843. doi:10.1002/ana.25620. https://pubmed.ncbi.nlm.nih.gov/31610034/
3. Ullah, Anwar, Lin, Zhaohan, Younus, Muhammad, Khan, Amjad, Umair, Muhammad. 2022. Homozygous missense variant in POPDC3 causes recessive limb-girdle muscular dystrophy type 26. In The journal of gene medicine, 24, e3412. doi:10.1002/jgm.3412. https://pubmed.ncbi.nlm.nih.gov/35075722/
4. He, Xu, Xu, Hongfa, Zhao, Wei, Tan, Li, Lu, Ligong. 2019. POPDC3 is a potential biomarker for prognosis and radioresistance in patients with head and neck squamous cell carcinoma. In Oncology letters, 18, 5468-5480. doi:10.3892/ol.2019.10888. https://pubmed.ncbi.nlm.nih.gov/31612055/
5. Luo, Deng, Lu, Ming-Liang, Zhao, Gong-Fang, Lv, Lin, Luo, Jin-Bo. . Reduced Popdc3 expression correlates with high risk and poor survival in patients with gastric cancer. In World journal of gastroenterology, 18, 2423-9. doi:10.3748/wjg.v18.i19.2423. https://pubmed.ncbi.nlm.nih.gov/22654436/
6. Zhang, Lin, Li, Wenwu, Weng, Yuting, Zhang, Xiaochao, Sun, Hao. 2022. A novel splice site variant in the POPDC3 causes autosomal recessive limb-girdle muscular dystrophy type 26. In Clinical genetics, 102, 345-349. doi:10.1111/cge.14192. https://pubmed.ncbi.nlm.nih.gov/35842834/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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