C57BL/6JCya-Alox8em1/Cya
Common Name
Alox8-KO
Product ID
S-KO-01009
Backgroud
C57BL/6JCya
Strain ID
KOCMP-11688-Alox8-B6J-VA
Status
When using this mouse strain in a publication, please cite “Alox8-KO Mouse (Catalog S-KO-01009) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Alox8-KO
Strain ID
KOCMP-11688-Alox8-B6J-VA
Gene Name
Product ID
S-KO-01009
Gene Alias
8-LOX, 8S-LOX, Alox15b, 15-LOX-2, 15-LOX-B
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 11
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000021262
NCBI RefSeq
NM_009661
Target Region
Exon 5~8
Size of Effective Region
~3.0 kb
Overview of Gene Research
Alox8, also known as murine Alox15b, is a lipoxygenase that catalyzes the peroxidation of arachidonic acid at carbon-8. It oxygenates polyunsaturated fatty acids in S-chirality with specific reaction specificity. Alox8 is involved in the arachidonate metabolism pathway and plays a role in regulating cholesterol homeostasis in macrophages [1].
In mouse models, Alox8 deficiency has been associated with reduced atherosclerosis [1]. Chromosome 11B3 deletions or Alox8 loss in mice lead to up-regulation of the cyclooxygenase pathway, increased levels of prostaglandin E2, and contribute to B-cell malignancy [2]. Alox8-deficient mice also show age-dependent impaired recovery from influenza infection [3]. Moreover, intermittent fasting in mice after traumatic brain injury can partly abolish the increase of Alox8 induced by TBI, alleviating ferroptosis-related cellular damage and improving cognitive function [4].
In conclusion, Alox8 is essential in arachidonate metabolism and impacts various biological processes. Mouse models with Alox8 deficiency have revealed its role in diseases such as atherosclerosis, B-cell lymphoma, and influenza recovery, as well as in neuronal ferroptosis after traumatic brain injury. These findings contribute to our understanding of the underlying mechanisms of these diseases and may provide potential therapeutic targets.
References:
1. Palmer, Megan A, Benatzy, Yvonne, Brüne, Bernhard. 2024. Murine Alox8 versus the human ALOX15B ortholog: differences and similarities. In Pflugers Archiv : European journal of physiology, 476, 1817-1832. doi:10.1007/s00424-024-02961-w. https://pubmed.ncbi.nlm.nih.gov/38637408/
2. Qi, Lu, Pan, Xiangyu, Chen, Xuelan, Liu, Yu, Xu, Zhengmin. 2023. COX-2/PGE2 upregulation contributes to the chromosome 17p-deleted lymphoma. In Oncogenesis, 12, 5. doi:10.1038/s41389-023-00451-9. https://pubmed.ncbi.nlm.nih.gov/36750552/
3. Alfardan, Rana, Guo, Changxiong, Toth, Linda A, Nie, Daotai. 2019. Impaired Recovery from Influenza A/X-31(H3N2) Infection in Mice with 8-Lipoxygenase Deficiency. In Medical sciences (Basel, Switzerland), 7, . doi:10.3390/medsci7040060. https://pubmed.ncbi.nlm.nih.gov/31013822/
4. Yang, Qiuyun, Li, Manrui, Liu, Jinyuan, Chen, Xiameng, Liang, Weibo. 2023. Intermittent fasting ameliorates neuronal ferroptosis and cognitive impairment in mice after traumatic brain injury. In Nutrition (Burbank, Los Angeles County, Calif.), 109, 111992. doi:10.1016/j.nut.2023.111992. https://pubmed.ncbi.nlm.nih.gov/36871445/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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