C57BL/6NCya-Fgaem1/Cya
Common Name
Fga-KO
Product ID
S-KO-02034
Backgroud
C57BL/6NCya
Strain ID
KOCMP-14161-Fga-B6N-VA
When using this mouse strain in a publication, please cite “Fga-KO Mouse (Catalog S-KO-02034) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Fga-KO
Strain ID
KOCMP-14161-Fga-B6N-VA
Gene Name
Product ID
S-KO-02034
Gene Alias
Fib
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 3
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000166581
NCBI RefSeq
NM_001111048
Target Region
Exon 2~5
Size of Effective Region
~3.5 kb
Overview of Gene Research
Fga, also known as fibrinogen alpha chain, is a crucial component of fibrinogen, an important plasma protein composed of three polypeptide chains (Fga, beta, and gamma). Fibrinogen is not only an inflammation regulator but also involved in various biological processes such as tumor progression. Fga has been associated with pathways like PI3K/AKT, VEGFR2-FAK, and FAK/ERK, highlighting its significance in different cellular functions [1,2,3].
In hepatocellular carcinoma (HCC), Fga was found to be downregulated and correlated with tumor stage and grade. Gene knockout and overexpression cell models demonstrated that overexpressing Fga inhibited migration and invasion of liver cancer cells, increased E-cadherin expression, decreased N-cadherin and slug protein expression, and knockout of Fga activated the PI3K/AKT pathway. In a mouse model of metastatic tumors, Fga overexpression restricted the spread of tumor cells, indicating its inhibitory effect on tumor metastasis [1]. In gastric cancer, low expression of Fga was observed in tissues and cell lines. Fga suppressed cell proliferation, motility, and EMT process, stimulated cell autophagy, and inhibited the FAK/ERK pathway through suppressing ITGA5 expression, and in vivo assays in BALB/c nude mice confirmed its role in suppressing tumor growth [3].
In conclusion, Fga plays a significant role in tumor-related biological processes. Studies using gene knockout and overexpression models in liver and gastric cancer have revealed its potential as a tumor-suppressing factor, providing new insights for the treatment of these cancers.
References:
1. Han, Xi, Liu, Zefeng, Cui, Mengying, Sheng, Jiyao, Zhang, Xuewen. 2024. FGA influences invasion and metastasis of hepatocellular carcinoma through the PI3K/AKT pathway. In Aging, 16, 12806-12819. doi:10.18632/aging.206011. https://pubmed.ncbi.nlm.nih.gov/39227068/
2. Li, Hui, Cai, E, Cheng, Hongyan, Zhu, Honglan, Chang, Xiaohong. 2022. FGA Controls VEGFA Secretion to Promote Angiogenesis by Activating the VEGFR2-FAK Signalling Pathway. In Frontiers in endocrinology, 13, 791860. doi:10.3389/fendo.2022.791860. https://pubmed.ncbi.nlm.nih.gov/35498401/
3. Liu, Guangli, Xu, Xintao, Geng, Hui, Zou, Shenshan, Li, Xin. 2022. FGA inhibits metastases and induces autophagic cell death in gastric cancer via inhibiting ITGA5 to regulate the FAK/ERK pathway. In Tissue & cell, 76, 101767. doi:10.1016/j.tice.2022.101767. https://pubmed.ncbi.nlm.nih.gov/35257941/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.