C57BL/6NCya-Dnah2em1/Cya
Common Name
Dnah2-KO
Product ID
S-KO-09388
Backgroud
C57BL/6NCya
Strain ID
KOCMP-327954-Dnah2-B6N-VA
When using this mouse strain in a publication, please cite “Dnah2-KO Mouse (Catalog S-KO-09388) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Dnah2-KO
Strain ID
KOCMP-327954-Dnah2-B6N-VA
Gene Name
Product ID
S-KO-09388
Gene Alias
Dnhd3, Dnahc2, 4930564A01, D130094J20, 2900022L05Rik, D330014H01Rik
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 11
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000108659
NCBI RefSeq
NM_001081330
Target Region
Exon 5~28
Size of Effective Region
~41.2 kb
Overview of Gene Research
DNAH2, also known as Dynein Axonemal Heavy Chain 2, is crucial for sperm flagella formation and functions in the homologous recombination repair pathway. It is associated with multiple morphological abnormalities of the sperm flagella (MMAF) and Fanconi anemia (FA) [1,2,3,4]. Genetic models, like gene-knockout mouse models, are valuable for studying its functions.
In mouse models, Dnah2-null males are infertile. Dnah2-null sperm cells show absent, short, bent, coiled, and/or irregular flagella, consistent with the MMAF phenotype. The ultrastructure of flagella is severely disorganized, and the absence of DNAH2 compromises the expression of other axonemal components. In U2OS cells, down-regulation of DNAH2 increases cell sensitivity to DNA inter-strand crosslinks, reduces FANCD2 protein enrichment at DNA damage sites, and influences FANCD2 ubiquitination modification [1,3].
In conclusion, DNAH2 is essential for sperm flagella formation and plays a role in the homologous recombination repair pathway. Studies using gene-knockout mouse models have revealed its significance in male infertility related to MMAF and in the pathogenesis of Fanconi anemia.
References:
1. Chang, Lixian, Gao, Xingjie, Wang, Yuxia, Yuan, Weiping, Zhu, Xiaofan. 2021. DNAH2 facilitates the homologous recombination repair of Fanconi anemia pathway through modulating FANCD2 ubiquitination. In Blood science (Baltimore, Md.), 3, 71-77. doi:10.1097/BS9.0000000000000076. https://pubmed.ncbi.nlm.nih.gov/35402838/
2. Li, Yang, Sha, Yanwei, Wang, Xiong, Tang, Yunge, Lu, Zhongxian. 2019. DNAH2 is a novel candidate gene associated with multiple morphological abnormalities of the sperm flagella. In Clinical genetics, 95, 590-600. doi:10.1111/cge.13525. https://pubmed.ncbi.nlm.nih.gov/30811583/
3. Hwang, Jae Yeon, Nawaz, Shoaib, Choi, Jungmin, Ahmad, Wasim, Chung, Jean-Ju. 2021. Genetic Defects in DNAH2 Underlie Male Infertility With Multiple Morphological Abnormalities of the Sperm Flagella in Humans and Mice. In Frontiers in cell and developmental biology, 9, 662903. doi:10.3389/fcell.2021.662903. https://pubmed.ncbi.nlm.nih.gov/33968937/
4. Gao, Yang, Tian, Shixiong, Sha, Yanwei, Zhang, Feng, He, Xiaojin. 2021. Novel bi-allelic variants in DNAH2 cause severe asthenoteratozoospermia with multiple morphological abnormalities of the flagella. In Reproductive biomedicine online, 42, 963-972. doi:10.1016/j.rbmo.2021.01.011. https://pubmed.ncbi.nlm.nih.gov/33771466/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.