C57BL/6JCya-Cdc23em1/Cya
Common Name:
Cdc23-KO
Product ID:
S-KO-17515
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Cdc23-KO
Strain ID
KOCMP-52563-Cdc23-B6J-VB
Gene Name
Product ID
S-KO-17515
Gene Alias
6030435O18; D18Ertd243e
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
18
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cdc23em1/Cya mice (Catalog S-KO-17515) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000133181
NCBI RefSeq
NM_178347
Target Region
Exon 7~9
Size of Effective Region
~2.9 kb
Detailed Document
Overview of Gene Research
Cdc23, also known as Mcm10 in some contexts, is a core subunit of the anaphase - promoting complex or cyclosome (APC/C) and is involved in regulating mitosis in eukaryotes [4]. It also plays a role in DNA replication processes, such as promoting Cdc45 chromatin binding and participating in the activation of the pre - replicative complex [5,6]. Its functions are crucial for cell cycle progression and normal cellular development.
In disease - related studies, knockdown of Cdc23 in liver cancer cell lines and xenograft models inhibited cell proliferation, migration, invasion, and tumor growth, likely through regulating the epithelial - mesenchymal transition (EMT) process, suggesting it could be a therapeutic target for liver cancer [1]. In female infertility cases, homozygous missense variants in Cdc23 led to oocyte maturation defects. Cdc23Y329C/Y329C mice mimicked the patient phenotype, with low expression of CDC23 and APC4 and accumulation of securin and cyclin B1 in oocytes, and AZ3146 treatment could rescue the phenotype [2]. In papillary thyroid cancer, functional knockdown of Cdc23 in thyroid cancer cells increased the number of cells in S and G(2)M phases, inhibited cellular proliferation, tumor spheroid formation, and anchorage - independent growth, indicating its role in thyroid cancer initiation and progression [3].
In conclusion, Cdc23 is essential for cell cycle regulation, DNA replication, and normal development. Studies using gene knockdown or mutant mouse models have revealed its significance in diseases like liver cancer, female infertility, and papillary thyroid cancer, providing insights into potential therapeutic strategies for these conditions.
References:
1. Zhang, Yang, Luo, Lianghua, Fu, Chengchao, Li, Yong, Xiong, Jianbo. 2023. CDC23 knockdown suppresses the proliferation, migration and invasion of liver cancer via the EMT process. In Oncology letters, 26, 291. doi:10.3892/ol.2023.13877. https://pubmed.ncbi.nlm.nih.gov/37274472/
2. Fan, Huizhen, Zhou, Zhou, Zheng, Wei, Sang, Qing, Wang, Lei. 2023. Homozygous variants in CDC23 cause female infertility characterized by oocyte maturation defects. In Human genetics, 142, 1621-1631. doi:10.1007/s00439-023-02606-5. https://pubmed.ncbi.nlm.nih.gov/37768355/
3. Zhang, Lisa, Rahbari, Reza, He, Mei, Kebebew, Electron. 2011. CDC23 regulates cancer cell phenotype and is overexpressed in papillary thyroid cancer. In Endocrine-related cancer, 18, 731-42. doi:10.1530/ERC-11-0181. https://pubmed.ncbi.nlm.nih.gov/21990323/
4. Xie, Su, Liu, Quan, Fu, Chong, Han, Min, Li, Changchun. 2024. Molecular Regulation of Porcine Skeletal Muscle Development: Insights from Research on CDC23 Expression and Function. In International journal of molecular sciences, 25, . doi:10.3390/ijms25073664. https://pubmed.ncbi.nlm.nih.gov/38612477/
5. Gregan, Juraj, Lindner, Karola, Brimage, Lydia, Aves, Stephen J, Kearsey, Stephen E. 2003. Fission yeast Cdc23/Mcm10 functions after pre-replicative complex formation to promote Cdc45 chromatin binding. In Molecular biology of the cell, 14, 3876-87. doi:. https://pubmed.ncbi.nlm.nih.gov/12972571/
6. Lee, Joon-Kyu, Seo, Yeon-Soo, Hurwitz, Jerard. 2003. The Cdc23 (Mcm10) protein is required for the phosphorylation of minichromosome maintenance complex by the Dfp1-Hsk1 kinase. In Proceedings of the National Academy of Sciences of the United States of America, 100, 2334-9. doi:. https://pubmed.ncbi.nlm.nih.gov/12604790/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen