C57BL/6JCya-Ccdc106em1/Cya
Common Name:
Ccdc106-KO
Product ID:
S-KO-17596
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ccdc106-KO
Strain ID
KOCMP-232821-Ccdc106-B6J-VB
Gene Name
Product ID
S-KO-17596
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ccdc106em1/Cya mice (Catalog S-KO-17596) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000108571
NCBI RefSeq
NM_001290429
Target Region
Exon 5~6
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
CCDC106, or coiled-coil domain-containing protein 106, has emerged as a significant player in multiple biological processes. It is involved in regulating the p53-Mdm2/MdmX signaling axis, which is crucial for maintaining the basal level of the tumor suppressor p53 [1]. By directly interacting with the p53 transactivation domain, CCDC106 competes with Mdm2 and MdmX, influencing the cellular levels of p53 and Mdm2/MdmX.
In cancer-related studies, CCDC106 knockdown enhanced apoptosis by stabilizing p53 and suppressed cell viability, colony formation, migration and invasion in cervical cancer HeLa and breast cancer MCF7 cells with wild-type p53, while overexpression had opposite effects in normal breast epithelial HBL100 and cervical cancer SiHa cells with wild-type p53 [5]. In ovarian cancer, overexpression of CCDC106 promoted proliferation, invasion and epithelial-to-mesenchymal transition (EMT) of mutant p53 ovarian cancer cells via the ATF4-mediated inhibition of p21 [2]. In non-small cell lung cancer, CCDC106 expression correlated with advanced TNM stage, positive regional lymph node metastasis and poor overall survival, and it promoted cell proliferation depending on AKT signaling [3]. Also, HPV-CCDC106 integration in cervical cancer altered local chromosome architecture, hijacked an enhancer, and promoted cancer progression by facilitating high CCDC106 expression after HPV E6 splicing, which led to p53 degradation [4,6].
In conclusion, CCDC106 has a significant impact on cancer-related biological processes, mainly through its interaction with p53 and involvement in cell-cycle-regulating and cancer-promoting pathways. Functional studies using gene knockout or knockdown models in various cancer cell lines have revealed its role in promoting cancer cell proliferation, invasion, and in some cases, p53 degradation. These findings suggest CCDC106 could be a potential therapeutic target in cancer treatment.
References:
1. Zhou, Ting, Ke, Zhiqiang, Ma, Qianqian, Cheng, Xiyao, Su, Zhengding. 2023. Molecular mechanism of CCDC106 regulating the p53-Mdm2/MdmX signaling axis. In Scientific reports, 13, 21892. doi:10.1038/s41598-023-47808-z. https://pubmed.ncbi.nlm.nih.gov/38081879/
2. Zhao, Na, Wang, Chen, Guo, Peng, Bhawal, Ujjal K, Liu, Yang. . CCDC106 promotes the proliferation and invasion of ovarian cancer cells by suppressing p21 transcription through a p53-independent pathway. In Bioengineered, 13, 10956-10972. doi:10.1080/21655979.2022.2066759. https://pubmed.ncbi.nlm.nih.gov/35484984/
3. Zhang, Xiupeng, Zheng, Qin, Wang, Chen, Qiu, Xueshan, Wang, Enhua. . CCDC106 promotes non-small cell lung cancer cell proliferation. In Oncotarget, 8, 26662-26670. doi:10.18632/oncotarget.15792. https://pubmed.ncbi.nlm.nih.gov/28460455/
4. Cao, Canhui, Hong, Ping, Huang, Xingyu, Li, Guoliang, Wu, Peng. 2020. HPV-CCDC106 integration alters local chromosome architecture and hijacks an enhancer by three-dimensional genome structure remodeling in cervical cancer. In Journal of genetics and genomics = Yi chuan xue bao, 47, 437-450. doi:10.1016/j.jgg.2020.05.006. https://pubmed.ncbi.nlm.nih.gov/33023834/
5. Ning, Yichong, Wang, Chunqing, Liu, Xin, Zhou, Jianlin, Zhou, Chang. 2019. CK2-mediated CCDC106 phosphorylation is required for p53 degradation in cancer progression. In Journal of experimental & clinical cancer research : CR, 38, 131. doi:10.1186/s13046-019-1137-8. https://pubmed.ncbi.nlm.nih.gov/30885251/
6. Zhi, Wenhua, Wei, Ye, Lazare, Cordelle, Cao, Canhui, Wu, Peng. 2022. HPV-CCDC106 integration promotes cervical cancer progression by facilitating the high expression of CCDC106 after HPV E6 splicing. In Journal of medical virology, 95, e28009. doi:10.1002/jmv.28009. https://pubmed.ncbi.nlm.nih.gov/35854676/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen