C57BL/6JCya-Stk32cem1/Cya
Common Name:
Stk32c-KO
Product ID:
S-KO-17629
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Stk32c-KO
Strain ID
KOCMP-57740-Stk32c-B6J-VC
Gene Name
Product ID
S-KO-17629
Gene Alias
PKE; Pkek; YANK3
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Stk32cem1/Cya mice (Catalog S-KO-17629) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000016125
NCBI RefSeq
NM_021302
Target Region
Exon 3~4
Size of Effective Region
~3.5 kb
Detailed Document
Overview of Gene Research
Stk32C, a member of the serine/threonine protein kinase of AGC superfamily, was first found highly expressed in brain tissues. It may play roles in multiple biological processes, though its exact function remains to be fully elucidated. Some studies suggest its possible involvement in pathways related to cell proliferation, migration, and invasion, as well as in glycolysis regulation [3,2].
In cancer research, data from TCGA database showed that the Stk32C gene is overexpressed in bladder cancer and a number of other human tumors. Slicing of Stk32C inhibited tumor cell proliferation, migration and invasion in vitro, and knocking-down of Stk32C restricted the growth of tumor cells in mice, indicating its promoting role in tumor progression [3]. In triple-negative breast cancer, elevated Stk32C expression was associated with unfavorable prognosis in doxorubicin-treated patients. Depletion of Stk32C augmented the sensitivity of doxorubicin-resistant cells to doxorubicin, with the cytoplasmic subset of Stk32C mediating this effect mainly through glycolysis regulation [2].
In conclusion, Stk32C appears to be involved in cancer-related processes such as tumor cell growth, invasion, and drug resistance, especially in bladder and triple-negative breast cancers. Its role in these diseases highlights the potential of targeting Stk32C as a new therapeutic approach. Additionally, its association with other conditions like intellectual disability and mental disorders from methylation-related studies suggests a broader influence on human health, although further research is needed to fully understand its functions [1,4,5].
References:
1. Anazi, Shams, Maddirevula, Sateesh, Salpietro, Vincenzo, Faqeih, Eissa, Alkuraya, Fowzan S. 2017. Expanding the genetic heterogeneity of intellectual disability. In Human genetics, 136, 1419-1429. doi:10.1007/s00439-017-1843-2. https://pubmed.ncbi.nlm.nih.gov/28940097/
2. Xiao, Huawei, Liu, Lei, Huang, Shaoyan. 2024. STK32C modulates doxorubicin resistance in triple-negative breast cancer cells via glycolysis regulation. In Molecular and cellular biochemistry, 480, 459-471. doi:10.1007/s11010-024-04989-z. https://pubmed.ncbi.nlm.nih.gov/38507019/
3. Sun, Erlin, Liu, Kangkang, Zhao, Kun, Wang, Lining. 2018. Serine/threonine kinase 32C is overexpressed in bladder cancer and contributes to tumor progression. In Cancer biology & therapy, 20, 307-320. doi:10.1080/15384047.2018.1529098. https://pubmed.ncbi.nlm.nih.gov/30359551/
4. Dempster, Emma L, Wong, Chloe C Y, Lester, Kathryn J, Mill, Jonathan, Eley, Thalia C. 2014. Genome-wide methylomic analysis of monozygotic twins discordant for adolescent depression. In Biological psychiatry, 76, 977-83. doi:10.1016/j.biopsych.2014.04.013. https://pubmed.ncbi.nlm.nih.gov/24929637/
5. Starnawska, A, Hansen, C S, Sparsø, T, Werge, T, Weinsheimer, S. 2017. Differential DNA methylation at birth associated with mental disorder in individuals with 22q11.2 deletion syndrome. In Translational psychiatry, 7, e1221. doi:10.1038/tp.2017.181. https://pubmed.ncbi.nlm.nih.gov/28850114/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen