C57BL/6JCya-Trim72em1/Cya
Common Name:
Trim72-KO
Product ID:
S-KO-17963
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Trim72-KO
Strain ID
KOCMP-434246-Trim72-B6J-VA
Gene Name
Product ID
S-KO-17963
Gene Alias
MG53
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Trim72em1/Cya mice (Catalog S-KO-17963) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000081042
NCBI RefSeq
NM_001079932.3
Target Region
Exon 2~5
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Trim72, also known as MG53, is a member of the tripartite motif protein family and functions as an E3 ubiquitin ligase. It plays a crucial role in membrane repair, anti-inflammatory processes, and is involved in multiple signaling pathways such as FAK/Akt, STAT3/Notch-1, and PI3K-AKT [1,3,4]. It has significant biological importance in various physiological and pathological processes across multiple organs [1]. Genetic models, like gene knockout (KO) mouse models, have been valuable in studying Trim72's functions.
In Trim72 knockout mice, increased mortality, organ fungal burden, and kidney damage were observed after lethal Candida albicans infection, indicating its positive regulation of antifungal immunity [2]. In mdx mice (a model for Duchenne muscular dystrophy), decreased Trim72 expression was found in skeletal muscles, and overexpressing Trim72 via AAV-TRIM72 alleviated muscle inflammation by promoting mitophagy-mediated NLRP3 inflammasome inactivation [5]. In the context of colorectal cancer, lower Trim72 levels in liver metastases compared to primary tumors were associated with lymph node metastasis and advanced clinical stages, and Trim72 overexpression inhibited cell migration and epithelial-mesenchymal transition, while knockout increased these processes [3].
In conclusion, Trim72 has diverse essential biological functions including membrane repair, anti-inflammatory effects, and regulation of cell migration and immunity. KO mouse models have revealed its significance in diseases such as fungal infections, Duchenne muscular dystrophy, and colorectal cancer, providing valuable insights into disease mechanisms and potential therapeutic targets.
References:
1. Wang, Yong-Fei, An, Zi-Yi, Li, Jian-Wen, Dong, Zi-Kai, Jin, Wei-Lin. 2024. MG53/TRIM72: multi-organ repair protein and beyond. In Frontiers in physiology, 15, 1377025. doi:10.3389/fphys.2024.1377025. https://pubmed.ncbi.nlm.nih.gov/38681139/
2. Tan, Wang, Liu, Jiayu, Yu, Renlin, Lai, Xiaofei, Cao, Ju. 2024. Trim72 is a major host factor protecting against lethal Candida albicans infection. In PLoS pathogens, 20, e1012747. doi:10.1371/journal.ppat.1012747. https://pubmed.ncbi.nlm.nih.gov/39585917/
3. Faleti, Oluwasijibomi Damola, Gong, Yibing, Long, Jingyi, Yao, Jinke, Wu, Gongfa. 2024. TRIM72 inhibits cell migration and epithelial-mesenchymal transition by attenuating FAK/akt signaling in colorectal cancer. In Heliyon, 10, e37714. doi:10.1016/j.heliyon.2024.e37714. https://pubmed.ncbi.nlm.nih.gov/39315132/
4. Chen, Xu, Su, Jie, Feng, Jianyu, Qiu, Chen, Zheng, Qijun. 2019. TRIM72 contributes to cardiac fibrosis via regulating STAT3/Notch-1 signaling. In Journal of cellular physiology, 234, 17749-17756. doi:10.1002/jcp.28400. https://pubmed.ncbi.nlm.nih.gov/30820965/
5. Wu, Mengli, Li, Huan, He, Juanjuan, Liu, Yanmei, Zhang, Weixi. 2023. TRIM72 Alleviates Muscle Inflammation in mdx Mice via Promoting Mitophagy-Mediated NLRP3 Inflammasome Inactivation. In Oxidative medicine and cellular longevity, 2023, 8408574. doi:10.1155/2023/8408574. https://pubmed.ncbi.nlm.nih.gov/36713032/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen