C57BL/6JCya-Pank1em1/Cya
Common Name:
Pank1-KO
Product ID:
S-KO-18392
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Pank1-KO
Strain ID
KOCMP-75735-Pank1-B6J-VB
Gene Name
Product ID
S-KO-18392
Gene Alias
Pank; Pank1b
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pank1em1/Cya mice (Catalog S-KO-18392) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000036584
NCBI RefSeq
NM_001114339
Target Region
Exon 3
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Pank1, or pantothenate kinase 1, is a rate-limiting enzyme in CoA biosynthesis. CoA is an essential cofactor for intermediary metabolism, involved in processes like the tricarboxylic acid cycle, nutrient oxidation, histone acetylation, and synthesis of lipids, glycans, and haem [2].
In cardiac function studies, cardiomyocyte-specific Pank1 deletion (cmPank1-/-) in mice reduced PANK1 mRNA, protein, and CoA levels. When subjected to pressure overload, cmPank1-/-mice had more ventricular dilation and exacerbated cardiac fibrosis, with changes in fatty acid and ketone metabolism, suggesting Pank1's role in regulating fibrotic and metabolic processes during pressure overload [1]. In leptin-deficient mice, Pank1 deficiency reduced hyperglycaemia and hyperinsulinaemia, modified global metabolism, and improved aspects of the diabetic phenotype, although it did not affect insulin resistance [3].
In conclusion, Pank1 is crucial for CoA biosynthesis, which impacts various metabolic processes. Studies using Pank1 knockout mouse models have revealed its significance in cardiac function during pressure overload and in modifying the diabetic phenotype in leptin-deficient mice. These findings enhance our understanding of the role of Pank1 in disease-related metabolic and physiological processes.
References:
1. Audam, Timothy N, Howard, Caitlin M, Garrett, Lauren F, Jackowski, Suzanne, Jones, Steven P. 2021. Cardiac PANK1 deletion exacerbates ventricular dysfunction during pressure overload. In American journal of physiology. Heart and circulatory physiology, 321, H784-H797. doi:10.1152/ajpheart.00411.2021. https://pubmed.ncbi.nlm.nih.gov/34533403/
2. Barritt, Samuel A, DuBois-Coyne, Sarah E, Dibble, Christian C. 2024. Coenzyme A biosynthesis: mechanisms of regulation, function and disease. In Nature metabolism, 6, 1008-1023. doi:10.1038/s42255-024-01059-y. https://pubmed.ncbi.nlm.nih.gov/38871981/
3. Leonardi, Roberta, Rock, Charles O, Jackowski, Suzanne. 2014. Pank1 deletion in leptin-deficient mice reduces hyperglycaemia and hyperinsulinaemia and modifies global metabolism without affecting insulin resistance. In Diabetologia, 57, 1466-75. doi:10.1007/s00125-014-3245-5. https://pubmed.ncbi.nlm.nih.gov/24781151/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen