C57BL/6JCya-Cyp2e1em1/Cya
Common Name:
Cyp2e1-KO
Product ID:
S-KO-19027
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Cyp2e1-KO
Strain ID
KOCMP-13106-Cyp2e1-B6J-VA
Gene Name
Product ID
S-KO-19027
Gene Alias
CYPIIE1; Cyp2e
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cyp2e1em1/Cya mice (Catalog S-KO-19027) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000026552
NCBI RefSeq
NM_021282
Target Region
Exon 3~5
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Cyp2e1, a cytochrome P450 enzyme, is involved in the metabolism of alcohol and other xenobiotics. It contributes to the generation of reactive oxygen species, leading to oxidative stress, and is associated with various biological processes and disease conditions [1,2,3,4,5,6].
In the context of alcoholic liver disease (ALD), studies using gene knockout (KO) mouse models have revealed important roles of Cyp2e1. In ERRγ -inducible FGF23-related ALD, hepatic CYP2E1 induction in response to alcohol is FGF23 dependent, and FGF23-LKO mice display decreased hepatic CYP2E1 expression and improved ALD through reduced hepatocyte apoptosis and oxidative stress [2]. Also, ethanol-induced fatty liver and oxidant stress in wild-type mice were blunted in CYP2E1 KO mice but restored in CYP2E1 knockin mice, indicating that Cyp2e1 contributes to ethanol-induced oxidant stress and liver injury [3]. Additionally, Cyp2e1 knockout mice are resistant to alcohol-induced gut leakiness and liver inflammation, suggesting its role in alcohol-induced gut permeability changes associated with ALD [4].
In conclusion, Cyp2e1 plays a crucial role in alcohol-related oxidative stress, liver injury, and gut permeability changes, as demonstrated by KO mouse models. These findings in ALD and other related conditions enhance our understanding of the biological functions of Cyp2e1 and its implications in disease development, providing potential therapeutic targets for treating associated diseases.
References:
1. Sutti, Salvatore, Rigamonti, Cristina, Vidali, Matteo, Albano, Emanuele. 2014. CYP2E1 autoantibodies in liver diseases. In Redox biology, 3, 72-8. doi:10.1016/j.redox.2014.11.004. https://pubmed.ncbi.nlm.nih.gov/25462068/
2. Jung, Yoon Seok, Radhakrishnan, Kamalakannan, Hammad, Seddik, Dooley, Steven, Choi, Hueng-Sik. 2024. ERRγ-inducible FGF23 promotes alcoholic liver injury through enhancing CYP2E1 mediated hepatic oxidative stress. In Redox biology, 71, 103107. doi:10.1016/j.redox.2024.103107. https://pubmed.ncbi.nlm.nih.gov/38479224/
3. Cederbaum, Arthur I. . CYP2E1 potentiates toxicity in obesity and after chronic ethanol treatment. In Drug metabolism and drug interactions, 27, 125-44. doi:10.1515/dmdi-2012-0014. https://pubmed.ncbi.nlm.nih.gov/23104821/
4. Forsyth, Christopher B, Voigt, Robin M, Keshavarzian, Ali. 2014. Intestinal CYP2E1: A mediator of alcohol-induced gut leakiness. In Redox biology, 3, 40-6. doi:10.1016/j.redox.2014.10.002. https://pubmed.ncbi.nlm.nih.gov/25462064/
5. Mo, Qigui, Song, Chenchen, Hua, Yu, Wang, Wei, Liu, Aimei. 2024. CYP2E1 mediated deoxynivalenol-induced hepatocyte toxicity by regulating ferroptosis. In Toxicology, 508, 153923. doi:10.1016/j.tox.2024.153923. https://pubmed.ncbi.nlm.nih.gov/39147090/
6. Cederbaum, Arthur I. . CYP2E1--biochemical and toxicological aspects and role in alcohol-induced liver injury. In The Mount Sinai journal of medicine, New York, 73, 657-72. doi:. https://pubmed.ncbi.nlm.nih.gov/16878272/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen