C57BL/6JCya-Cdo1em1/Cya
Common Name:
Cdo1-KO
Product ID:
S-KO-19032
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Cdo1-KO
Strain ID
KOCMP-12583-Cdo1-B6J-VA
Gene Name
Product ID
S-KO-19032
Gene Alias
1300002L19Rik; Cdo; D18Ucla3
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
18
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cdo1em1/Cya mice (Catalog S-KO-19032) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000035804
NCBI RefSeq
NM_033037
Target Region
Exon 3
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
Cdo1, also known as cysteine dioxygenase type 1, is a key enzyme in cysteine catabolism and taurine synthesis, belonging to the mammalian non-heme Fe(II) dioxygenases family [3,4]. It is highly expressed in multiple tissues like liver, adipose tissue, pancreas, etc. Cdo1 is involved in numerous physiological processes such as lipid metabolism, adipogenesis, and redox homeostasis, and plays a role in pathophysiological processes like tumor progression [4]. Genetic models, such as gene knockout mouse models, are valuable for studying its functions.
In mice, hepatocyte-specific knockout of Cdo1 (Cdo1LKO) decreases basal metabolic rate and impairs exercise-induced protection against non-alcoholic fatty liver disease (NAFLD), while hepatocyte-specific overexpression (Cdo1LTG) increases basal metabolic rate and synergizes with exercise to ameliorate NAFLD. Cdo1 tethers Camkk2 to AMPK, activating AMPK signaling, promoting fatty acid oxidation and mitochondrial biogenesis in hepatocytes to attenuate hepatosteatosis [1]. Adipose-specific knockout of Cdo1 in mice impairs energy expenditure, cold tolerance, and lipolysis, exacerbates diet-induced obesity (DIO), and decreases adipose expression of key lipolytic genes, while transgenic overexpression of Cdo1 shows better cold tolerance, ameliorated DIO, and higher lipolysis capacity [2].
In conclusion, Cdo1 plays essential roles in lipid metabolism and energy regulation as revealed through mouse model-based research. In the context of NAFLD and obesity, Cdo1 knockout mouse models have demonstrated its significance in these disease areas, highlighting its potential as a therapeutic target for related metabolic disorders.
References:
1. Chen, Min, Zhu, Jie-Ying, Mu, Wang-Jing, Li, Ruo-Ying, Guo, Liang. 2023. Cdo1-Camkk2-AMPK axis confers the protective effects of exercise against NAFLD in mice. In Nature communications, 14, 8391. doi:10.1038/s41467-023-44242-7. https://pubmed.ncbi.nlm.nih.gov/38110408/
2. Guo, Ying-Ying, Li, Bai-Yu, Xiao, Gang, Guo, Liang, Tang, Qi-Qun. 2022. Cdo1 promotes PPARγ-mediated adipose tissue lipolysis in male mice. In Nature metabolism, 4, 1352-1368. doi:10.1038/s42255-022-00644-3. https://pubmed.ncbi.nlm.nih.gov/36253617/
3. Liu, Qi, Shen, Wen-Qing. . [Molecular mechanism of CDO1 regulating common metabolic diseases]. In Sheng li xue bao : [Acta physiologica Sinica], 76, 576-586. doi:. https://pubmed.ncbi.nlm.nih.gov/39192790/
4. Chen, Min, Zhu, Jie-Ying, Mu, Wang-Jing, Guo, Liang. 2022. Cysteine dioxygenase type 1 (CDO1): Its functional role in physiological and pathophysiological processes. In Genes & diseases, 10, 877-890. doi:10.1016/j.gendis.2021.12.023. https://pubmed.ncbi.nlm.nih.gov/37396540/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen