C57BL/6JCya-Tap1em1/Cya
Common Name:
Tap1-KO
Product ID:
S-KO-19084
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tap1-KO
Strain ID
KOCMP-21354-Tap1-B6J-VB
Gene Name
Product ID
S-KO-19084
Gene Alias
ABC17; APT1; Abcb2; Ham-1; Ham1; MTP1; PSF1; RING4; TAP; Tap-1; Y3
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tap1em1/Cya mice (Catalog S-KO-19084) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000170086
NCBI RefSeq
NM_013683
Target Region
Exon 4~5
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Tap1, also known as Antigen Peptide Transporter 1, is a member of the ATP-binding cassette (ABC) family. It is crucial for transporting antigen peptides from the cytoplasm to the lumen of the endoplasmic reticulum, and then loading them onto major histocompatibility complex (MHC) class I molecules, thus playing a significant role in the immune response and antigen presentation [5].
Tap1 has been associated with multiple diseases. In ankylosing spondylitis, specific polymorphisms in Tap1 (Tap1-333Val, Tap1-637Gly) were likely to be associated with the disease, especially when compared with HLA-B27-negative controls [1]. In uveal melanoma, upregulated Tap1 was associated with shorter survival, higher metastasis likelihood, and higher mortality, and in vitro silencing of Tap1 inhibited cell proliferation and metastasis [2]. In gastric cancer, Tap1 was identified as a T-cell related therapeutic target, and oxaliplatin could enhance immunotherapy by promoting Tap1 expression [3]. In colorectal cancer, down-regulation of Tap1 was a mechanism of tumor immune escape and a poor prognostic factor [4]. In pancreatic cancer, Tap1 promoted resistance to MEK inhibitors, and suppressing Tap1 sensitized resistant cells to the inhibitor [6]. In gastric carcinoma, TAP1 expression was positively correlated with various immunological traits and patients with high TAP1 expression were more likely to achieve complete remission after immunotherapy [5]. In locally advanced gastric cancer, high Tap1 expression was associated with better overall survival [7]. In a study with Apoe⁻/⁻Tap1⁻/⁻ mice, Tap1-deficiency did not alter atherosclerosis development, indicating a minor role for CD8⁺ T cells and Tap1-dependent antigen presentation in this disease process [8].
In conclusion, Tap1 is essential for antigen presentation and immune response. Studies, especially those using gene-knockout mouse models like Apoe⁻/⁻Tap1⁻/⁻ mice, have revealed its diverse roles in multiple diseases, including autoimmune diseases, cancers, and atherosclerosis. These findings contribute to a better understanding of disease mechanisms and may provide potential therapeutic targets.
References:
1. Qian, Yufeng, Wang, Genlin, Xue, Feng, Tang, Liang, Yang, Huilin. 2017. Genetic association between TAP1 and TAP2 polymorphisms and ankylosing spondylitis: a systematic review and meta-analysis. In Inflammation research : official journal of the European Histamine Research Society ... [et al.], 66, 653-661. doi:10.1007/s00011-017-1047-1. https://pubmed.ncbi.nlm.nih.gov/28405734/
2. Zhu, Ru, Chen, Yu-Ting, Wang, Bo-Wen, Jiang, Fa-Gang, Zhang, Ming-Chang. 2023. TAP1, a potential immune-related prognosis biomarker with functional significance in uveal melanoma. In BMC cancer, 23, 146. doi:10.1186/s12885-023-10527-9. https://pubmed.ncbi.nlm.nih.gov/36774490/
3. Zhao, Yupeng, Liu, Ziyuan, Deng, Kaiyuan, Li, Ranran, Xia, Jiazeng. 2024. Identification of TAP1 as a T-cell related therapeutic target in gastric cancer by mediating oxalipliatin-related synergistic enhancement of immunotherapy. In International immunopharmacology, 132, 111998. doi:10.1016/j.intimp.2024.111998. https://pubmed.ncbi.nlm.nih.gov/38593510/
4. Ling, Agnes, Löfgren-Burström, Anna, Larsson, Pär, Edin, Sofia, Palmqvist, Richard. 2017. TAP1 down-regulation elicits immune escape and poor prognosis in colorectal cancer. In Oncoimmunology, 6, e1356143. doi:10.1080/2162402X.2017.1356143. https://pubmed.ncbi.nlm.nih.gov/29147604/
5. He, Zehua, Yang, Hong, Chen, Qingfeng, He, Wanrong, Chen, Zhihui. 2024. Role of TAP1 in the identification of immune-hot tumor microenvironment and its prognostic significance for immunotherapeutic efficacy in gastric carcinoma. In Journal of gastrointestinal oncology, 15, 890-907. doi:10.21037/jgo-24-28. https://pubmed.ncbi.nlm.nih.gov/38989426/
6. Li, Boya, Feng, Yu, Hou, Qiaoyun, Fu, Yan, Luo, Yongzhang. 2022. Antigen Peptide Transporter 1 (TAP1) Promotes Resistance to MEK Inhibitors in Pancreatic Cancers. In International journal of molecular sciences, 23, . doi:10.3390/ijms23137168. https://pubmed.ncbi.nlm.nih.gov/35806187/
7. Segami, Kenki, Aoyama, Toru, Hiroshima, Yukihiko, Saeki, Hiroshi, Oshima, Takashi. . Clinical Significance of TAP1 and DLL4 Expression in Patients With Locally Advanced Gastric Cancer. In In vivo (Athens, Greece), 35, 2771-2777. doi:10.21873/invivo.12562. https://pubmed.ncbi.nlm.nih.gov/34410967/
8. Kolbus, Daniel, Ljungcrantz, Irena, Söderberg, Ingrid, Nilsson, Jan, Fredrikson, Gunilla Nordin. 2012. TAP1-deficiency does not alter atherosclerosis development in Apoe-/- mice. In PloS one, 7, e33932. doi:10.1371/journal.pone.0033932. https://pubmed.ncbi.nlm.nih.gov/22479479/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen