C57BL/6JCya-Gpx3em1/Cya
Common Name:
Gpx3-KO
Product ID:
S-KO-19356
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Gpx3-KO
Strain ID
KOCMP-14778-Gpx3-B6J-VB
Gene Name
Product ID
S-KO-19356
Gene Alias
EGPx; GPx; GSHPx-3; GSHPx-P
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gpx3em1/Cya mice (Catalog S-KO-19356) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000082430
NCBI RefSeq
NM_008161
Target Region
Exon 2~5
Size of Effective Region
~2.8 kb
Detailed Document
Overview of Gene Research
Gpx3, glutathione peroxidase 3, is a selenoprotein and the only extracellular GPx in a family of oxidoreductases. It catalyzes the detoxification of hydro-and soluble lipid hydroperoxides by reduced glutathione, playing a crucial role in the antioxidant defense system. It is involved in maintaining redox balance, which is essential for normal cell function and survival [2].
In cancer research, conditional knockout of Gpx3 in AT2 cells suppressed lung metastasis in spontaneous metastatic models, revealing its role in promoting pre-metastatic niche formation in lung metastasis [1]. In ovarian cancer, knockdown of Gpx3 in ID8 cells abrogated clonogenicity and intraperitoneal tumor development in vivo, and in OVCAR3 cells, loss of Gpx3 led to decreased gene expression patterns related to pro-tumorigenic signaling pathways [4].
In cardiomyocytes, Gpx3 suppression increased ROS levels, induced apoptosis, and promoted autophagy [3]. In a murine model of pulmonary artery banding, GPx3-deficient mice showed enhanced adverse right ventricular remodeling and signs of RV dysfunction [5].
In chronic kidney disease models, down-regulation of Gpx3 in renal tubular epithelium led to an oxidatively stressed extracellular microenvironment, driving interstitial fibroblast activation and proliferation [6].
In conclusion, Gpx3 is vital for antioxidant defense and redox balance. Gene knockout models, especially in cancer, cardiomyocyte, and right ventricle-related disease models, have revealed its role in tumor progression, cell apoptosis, autophagy, and organ-specific remodeling. These studies provide insights into disease mechanisms and potential therapeutic targets related to Gpx3 [1,3,4,5,6].
References:
1. Wang, Zixin, Zhu, Jie, Liu, Yanfang, Cao, Xuetao, Gu, Yan. 2022. Tumor-polarized GPX3+ AT2 lung epithelial cells promote premetastatic niche formation. In Proceedings of the National Academy of Sciences of the United States of America, 119, e2201899119. doi:10.1073/pnas.2201899119. https://pubmed.ncbi.nlm.nih.gov/35914155/
2. Chang, Caroline, Worley, Beth L, Phaëton, Rébécca, Hempel, Nadine. 2020. Extracellular Glutathione Peroxidase GPx3 and Its Role in Cancer. In Cancers, 12, . doi:10.3390/cancers12082197. https://pubmed.ncbi.nlm.nih.gov/32781581/
3. Gong, Yafan, Yang, Jie, Cai, Jingzeng, Zhang, Jun Min, Zhang, Ziwei. 2018. Effect of Gpx3 gene silencing by siRNA on apoptosis and autophagy in chicken cardiomyocytes. In Journal of cellular physiology, 234, 7828-7838. doi:10.1002/jcp.27842. https://pubmed.ncbi.nlm.nih.gov/30515791/
4. Chang, Caroline, Cheng, Ya-Yun, Kamlapurkar, Shriya, Phaëton, Rébécca, Hempel, Nadine. 2024. GPX3 supports ovarian cancer tumor progression in vivo and promotes expression of GDF15. In Gynecologic oncology, 185, 8-16. doi:10.1016/j.ygyno.2024.02.004. https://pubmed.ncbi.nlm.nih.gov/38342006/
5. Covington, Taylor A, Pilz, Patrick M, Mulhern, Ryan M, Fisch, Sudeshna, Grune, Jana. 2023. GPx3 deficiency exacerbates maladaptive right ventricular remodeling in experimental pulmonary artery banding. In American journal of physiology. Lung cellular and molecular physiology, 324, L550-L556. doi:10.1152/ajplung.00379.2022. https://pubmed.ncbi.nlm.nih.gov/36880685/
6. Li, Li, Lu, Meizhi, Peng, Yiling, Zhou, Lili, Liu, Youhua. 2023. Oxidatively stressed extracellular microenvironment drives fibroblast activation and kidney fibrosis. In Redox biology, 67, 102868. doi:10.1016/j.redox.2023.102868. https://pubmed.ncbi.nlm.nih.gov/37690165/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen