C57BL/6JCya-Cbx5em1/Cya
Common Name:
Cbx5-KO
Product ID:
S-KO-19432
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Cbx5-KO
Strain ID
KOCMP-12419-Cbx5-B6J-VB
Gene Name
Product ID
S-KO-19432
Gene Alias
2610029O15Rik; HP1; Hp1a; Hp1alpha
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
15
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cbx5em1/Cya mice (Catalog S-KO-19432) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000118152
NCBI RefSeq
NM_001076789
Target Region
Exon 3
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
CBX5, also known as HP1α, is an epigenetic regulatory protein. It is involved in maintaining chromatin state, as it can recognize histone 3 lysine trimethylation at residue K9 (H3K9me3), a transcriptionally suppressive epigenetic mark, and is associated with the formation of inaccessible heterochromatin and suppression of gene expression [6].
In myeloid leukemia, the RNA binding proteins RBMX and RBMXL1 control leukemia cell survival by regulating chromatin state through their downstream target CBX5. Loss of RBMX/L1 leads to significant chromatin changes, and forced CBX5 expression can rescue the effects of RBMX/L1 depletion on cell growth and apoptosis [1].
In lung cancer, loss of CBX5 drives EGFR inhibitor resistance. CBX5 loss increases the expression of E2F1, which in turn upregulates the anti-apoptotic gene BIRC5, attenuating apoptosis induction following EGFRi treatment [2].
In periodontitis, Porphyromonas gingivalis OMVs contain msRNA sRNA45033 that targets CBX5, and CBX5 regulates apoptosis through the methylation of p53 DNA [3].
In lung fibrosis, CBX5, along with histone methyltransferase G9a, is essential for fibroblast activation by epigenetically repressing the peroxisome proliferator-activated receptor gamma coactivator 1 alpha gene (PPARGC1A). Inhibition of the CBX5/G9a pathway reduces collagen accumulation in the lungs following bleomycin injury [4]. Targeting CBX5 promotes fibroblast metabolic reprogramming and inhibits lung fibrosis, as loss of mesenchymal CBX5 attenuates fibrosis development, accompanied by changes in the expression of pathogenic fibroblast and metabolic genes [5].
In glioma, the lncRNA LINC02381 can interact with CEBPβ to transcriptionally enhance CBX5, promoting cell proliferation and hindering apoptosis [7].
In summary, CBX5 plays crucial roles in multiple biological processes and disease conditions. Through gene knockout or conditional knockout mouse models and other functional studies, its functions in maintaining chromatin state, cell growth, apoptosis, and disease-related pathways have been revealed. These findings highlight its potential as a therapeutic target in myeloid malignancies, lung cancer, periodontitis, and lung fibrosis.
References:
1. Prieto, Camila, Nguyen, Diu T T, Liu, Zhaoqi, Rabadan, Raul, Kharas, Michael G. 2021. Transcriptional control of CBX5 by the RNA binding proteins RBMX and RBMXL1 maintains chromatin state in myeloid leukemia. In Nature cancer, 2, 741-757. doi:10.1038/s43018-021-00220-w. https://pubmed.ncbi.nlm.nih.gov/34458856/
2. Bugide, Suresh, Edwards, Yvonne J K, Gupta, Romi, Green, Michael R, Wajapeyee, Narendra. 2023. CBX5 loss drives EGFR inhibitor resistance and results in therapeutically actionable vulnerabilities in lung cancer. In Proceedings of the National Academy of Sciences of the United States of America, 120, e2218118120. doi:10.1073/pnas.2218118120. https://pubmed.ncbi.nlm.nih.gov/36652476/
3. Fan, Ruyi, Zhou, Yi, Chen, Xu, Wang, Xiaoqian, Xu, Yan. 2023. Porphyromonas gingivalis Outer Membrane Vesicles Promote Apoptosis via msRNA-Regulated DNA Methylation in Periodontitis. In Microbiology spectrum, 11, e0328822. doi:10.1128/spectrum.03288-22. https://pubmed.ncbi.nlm.nih.gov/36629433/
4. Ligresti, Giovanni, Caporarello, Nunzia, Meridew, Jeffrey A, Urrutia, Raul A, Tschumperlin, Daniel J. 2019. CBX5/G9a/H3K9me-mediated gene repression is essential to fibroblast activation during lung fibrosis. In JCI insight, 5, . doi:10.1172/jci.insight.127111. https://pubmed.ncbi.nlm.nih.gov/31095524/
5. Hong, Jeongmin, Pham, Tho X, Lee, Jisu, Huang, Steven K, Ligresti, Giovanni. 2024. Targeting the Epigenetic Regulator CBX5 Promotes Fibroblast Metabolic Reprogramming and Inhibits Lung Fibrosis. In American journal of respiratory cell and molecular biology, , . doi:10.1165/rcmb.2024-0255OC. https://pubmed.ncbi.nlm.nih.gov/39642371/
6. van Wijnen, Andre J, Bagheri, Leila, Badreldin, Amr A, Paradise, Christopher R, Wu, Zhong. 2020. Biological functions of chromobox (CBX) proteins in stem cell self-renewal, lineage-commitment, cancer and development. In Bone, 143, 115659. doi:10.1016/j.bone.2020.115659. https://pubmed.ncbi.nlm.nih.gov/32979540/
7. Sun, Yong, Wang, Xinjun, Bu, Xingyao. 2021. LINC02381 contributes to cell proliferation and hinders cell apoptosis in glioma by transcriptionally enhancing CBX5. In Brain research bulletin, 176, 121-129. doi:10.1016/j.brainresbull.2021.07.009. https://pubmed.ncbi.nlm.nih.gov/34274429/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen