Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Cbx5em1/Cya
Common Name:
Cbx5-KO
Product ID:
S-KO-19432
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Cbx5-KO
Strain ID
KOCMP-12419-Cbx5-B6J-VB
Gene Name
Cbx5
Product ID
S-KO-19432
Gene Alias
2610029O15Rik; HP1; Hp1a; Hp1alpha
Background
C57BL/6JCya
NCBI ID
12419
Modification
Conventional knockout
Chromosome
15
Phenotype
MGI:109372
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cbx5em1/Cya mice (Catalog S-KO-19432) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000118152
NCBI RefSeq
NM_001076789
Target Region
Exon 3
Size of Effective Region
~1.4 kb
Detailed Document
Click here to download >>
Overview of Gene Research
CBX5, also known as HP1α, is an epigenetic regulatory protein. It is involved in maintaining chromatin state, as it can recognize histone 3 lysine trimethylation at residue K9 (H3K9me3), a transcriptionally suppressive epigenetic mark, and is associated with the formation of inaccessible heterochromatin and suppression of gene expression [6].

In myeloid leukemia, the RNA binding proteins RBMX and RBMXL1 control leukemia cell survival by regulating chromatin state through their downstream target CBX5. Loss of RBMX/L1 leads to significant chromatin changes, and forced CBX5 expression can rescue the effects of RBMX/L1 depletion on cell growth and apoptosis [1].

In lung cancer, loss of CBX5 drives EGFR inhibitor resistance. CBX5 loss increases the expression of E2F1, which in turn upregulates the anti-apoptotic gene BIRC5, attenuating apoptosis induction following EGFRi treatment [2].

In periodontitis, Porphyromonas gingivalis OMVs contain msRNA sRNA45033 that targets CBX5, and CBX5 regulates apoptosis through the methylation of p53 DNA [3].

In lung fibrosis, CBX5, along with histone methyltransferase G9a, is essential for fibroblast activation by epigenetically repressing the peroxisome proliferator-activated receptor gamma coactivator 1 alpha gene (PPARGC1A). Inhibition of the CBX5/G9a pathway reduces collagen accumulation in the lungs following bleomycin injury [4]. Targeting CBX5 promotes fibroblast metabolic reprogramming and inhibits lung fibrosis, as loss of mesenchymal CBX5 attenuates fibrosis development, accompanied by changes in the expression of pathogenic fibroblast and metabolic genes [5].

In glioma, the lncRNA LINC02381 can interact with CEBPβ to transcriptionally enhance CBX5, promoting cell proliferation and hindering apoptosis [7].

In summary, CBX5 plays crucial roles in multiple biological processes and disease conditions. Through gene knockout or conditional knockout mouse models and other functional studies, its functions in maintaining chromatin state, cell growth, apoptosis, and disease-related pathways have been revealed. These findings highlight its potential as a therapeutic target in myeloid malignancies, lung cancer, periodontitis, and lung fibrosis.

References:
1. Prieto, Camila, Nguyen, Diu T T, Liu, Zhaoqi, Rabadan, Raul, Kharas, Michael G. 2021. Transcriptional control of CBX5 by the RNA binding proteins RBMX and RBMXL1 maintains chromatin state in myeloid leukemia. In Nature cancer, 2, 741-757. doi:10.1038/s43018-021-00220-w. https://pubmed.ncbi.nlm.nih.gov/34458856/
2. Bugide, Suresh, Edwards, Yvonne J K, Gupta, Romi, Green, Michael R, Wajapeyee, Narendra. 2023. CBX5 loss drives EGFR inhibitor resistance and results in therapeutically actionable vulnerabilities in lung cancer. In Proceedings of the National Academy of Sciences of the United States of America, 120, e2218118120. doi:10.1073/pnas.2218118120. https://pubmed.ncbi.nlm.nih.gov/36652476/
3. Fan, Ruyi, Zhou, Yi, Chen, Xu, Wang, Xiaoqian, Xu, Yan. 2023. Porphyromonas gingivalis Outer Membrane Vesicles Promote Apoptosis via msRNA-Regulated DNA Methylation in Periodontitis. In Microbiology spectrum, 11, e0328822. doi:10.1128/spectrum.03288-22. https://pubmed.ncbi.nlm.nih.gov/36629433/
4. Ligresti, Giovanni, Caporarello, Nunzia, Meridew, Jeffrey A, Urrutia, Raul A, Tschumperlin, Daniel J. 2019. CBX5/G9a/H3K9me-mediated gene repression is essential to fibroblast activation during lung fibrosis. In JCI insight, 5, . doi:10.1172/jci.insight.127111. https://pubmed.ncbi.nlm.nih.gov/31095524/
5. Hong, Jeongmin, Pham, Tho X, Lee, Jisu, Huang, Steven K, Ligresti, Giovanni. 2024. Targeting the Epigenetic Regulator CBX5 Promotes Fibroblast Metabolic Reprogramming and Inhibits Lung Fibrosis. In American journal of respiratory cell and molecular biology, , . doi:10.1165/rcmb.2024-0255OC. https://pubmed.ncbi.nlm.nih.gov/39642371/
6. van Wijnen, Andre J, Bagheri, Leila, Badreldin, Amr A, Paradise, Christopher R, Wu, Zhong. 2020. Biological functions of chromobox (CBX) proteins in stem cell self-renewal, lineage-commitment, cancer and development. In Bone, 143, 115659. doi:10.1016/j.bone.2020.115659. https://pubmed.ncbi.nlm.nih.gov/32979540/
7. Sun, Yong, Wang, Xinjun, Bu, Xingyao. 2021. LINC02381 contributes to cell proliferation and hinders cell apoptosis in glioma by transcriptionally enhancing CBX5. In Brain research bulletin, 176, 121-129. doi:10.1016/j.brainresbull.2021.07.009. https://pubmed.ncbi.nlm.nih.gov/34274429/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest